Investigação dos polimorfismos GSTM1 e GSTT1 em mulheres com endometriose

• Ano de defesa: 2011
• Autor(a) principal: Frare, Ariane Bocaletto
• Orientador(a): Moura, Katia Karina Verolli de Oliveira
• Banca de defesa: Reis, Angela Adamski da Silva , Bordin, Bárbara Mariotto , Barcelos, Rejane da Silva Sena
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica de Goiás
• Programa de Pós-Graduação: Genética
• Departamento: Ciências Humanas
• País: BR
• Palavras-chave em Português: Endometriose; infertilidade; glutationa S-transferase; GSTM1; GSTT1
• Palavras-chave em Inglês: Endometriosis; infertility; glutathione S-transferase; GSTM1; GSTT1
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: https://tede2.pucgoias.edu.br/handle/tede/2337

Endometriosis is a benign gynecological pathology dependent on estrogen, which affects 10% of women of reproductive age, it is characterized by the presence of endometrial tissue outside the uterine cavity. Its etiology remains unknown, thus an increasing number of researches have been conducted in the search for associations between endometriosis and alterations or polymorphisms in candidate genes, among these genes are the GSTM1 and GSTT1. This study aimed to verify the possible correlation between endometriosis and polymorphisms of presence / absence of GSTM1 and GSTT1, and the association between GSTM1 and GSTT1 polymorphisms and p53 Arg / Pro. We analyzed the frequency of GSTM1 and GSTT1 polymorphisms in 50 women diagnosed with endometriosis and in control group with 46 women without complaints related to this pathology, the genotypes for these polymorphisms were determined using PCR. We found an increased frequency of absence of GSTM1 (61%) and presence of GSTT1 genes (55%) in the group of women studied, which corroborate with the literature. When we relate the endometriosis with the studied polymorphisms we verified that the endometriosis group had a higher presence of both polymorphisms 50% of GTSM1 and 68% of GSTT1 whereas in the control group there was the opposite, a higher absence of polymorphisms, 74% of GSTM1 and 59% of GSTT1, showing that these polymorphisms has no relation to the proliferation of endometriosis, however the analysis of the pathology with the association of GSTM1, GSTT1 polymorphisms with the p53 codon 72 gene was statistically significant.