Potencial genotóxico e mutagênico do bisfenol A-glicidil metacrilato (BisGMA - resina composta odontológica) em células do tecido meristemático radicular de Allium cepa

• Ano de defesa: 2024
• Autor(a) principal: Freitas, Marina Morais
• Orientador(a): Silva, Cláudio Carlos da
• Banca de defesa: Ayres, Flávio Monteiro , Minasi, Lysa Bernardes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica de Goiás
• Programa de Pós-Graduação: Programa de Pós-Graduação STRICTO SENSU em Genética
• Departamento: Escola de Ciências Médicas e da Vida
• País: Brasil
• Palavras-chave em Português: Resina composta; Allium cepa; BisGMA; Toxicidade
• Palavras-chave em Inglês: Composite resin; Toxicity
• Área do conhecimento CNPq: CIÊNCIAS BIOLÓGICAS::GENÉTICA
• Link de acesso: https://tede2.pucgoias.edu.br/handle/tede/5239

The genotoxicity and mutagenicity potential of a substance can be assessed using cellular analysis and the mitotic index of dividing cells during the cell cycle of a test organism, as well as by analyzing events such as chromatid breaks, loss of entire chromosomes and their fragments, and the formation of micronuclei. Investigation of genotoxicity in plants has emerged as a viable strategy for evaluating the mutagenic potential of substances in therapies, as well as for monitoring environmental quality. Among the various bioassays used, the Allium cepa system stands out due to its simplified methodology, reduced operational costs, and efficacy validated by international bodies. The objective of this study was to evaluate the toxicity and genotoxicity of bisphenol A - glycidyl methacrylate (BisGMA), a composite resin widely used in dentistry. Despite its popularity, recent studies have raised concerns about its safety, including potential toxic and genotoxic effects, highlighting the need for investigation. In this study, three different concentrations of BisGMA composite resin - Opallis Flow® color A1 diluted in water heated up to 60°C were used, employing the Allium cepa test. Additionally, a negative control with mineral water and a positive control with Paracetamol 750 mg were used. Tests with Allium cepa revealed significant variations in mitotic indices in response to treatments, with solution B (BisGMA concentration of 5 mg/mL in distilled water) showing noteworthy results. Chromosomal aberrations such as migration errors and micronucleated cells were observed in all treatments except solution B after 24 hours. Although statistically significant differences were not found between treatments and controls, the results indicate the need for further studies to deepen the understanding of the genotoxic potential of the substances tested.