Avaliação in vitro do manganês associado ao ácido hialurônico contra a citotoxicidade induzida pelo veneno da Bothrops jararacussu

• Ano de defesa: 2024
• Autor(a) principal: Carvalho, Filipe Feitosa de
• Orientador(a): Moura, Priscila Randazzo de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica de São Paulo
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biomateriais e Medicina Regenerativa
• Departamento: Faculdade de Ciências Médicas e da Saúde
• País: Brasil
• Palavras-chave em Português: Bothrops jararacussu; Manganês; Ácido hialurônico; Citotoxicidade; Viabilidade celular
• Palavras-chave em Inglês: Bothrops jararacussu; Manganese; Hyaluronic acid; Cytotoxicity; Cell viability
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.pucsp.br/jspui/handle/handle/42877

Snakebite accidents represent a serious public health issue, particularly in rural areas with limited access to medical care. In Brazil, the Bothrops genus is responsible for a significant portion of envenomation cases, with Bothrops jararacussu being particularly noteworthy. Although antivenom therapy is the primary treatment, local damage such as myotoxicity and tissue necrosis are often irreversible. This study evaluated the potential of manganese ion (Mn² + ) combined with hyaluronic acid (HA) as a complementary therapy to reduce the cytotoxicity caused by Bothrops jararacussu (Bjssu) venom in murine myoblast cultures. Cell viability assays were conducted using the MTT test, where myoblasts were exposed to different concentrations of Mn² + (1, 2, 4, 8, and 16 mM) either alone or combined with HA (2500 µg/mL), Bjssu (1.25, 2.5, 5, 10, 20, and 40 µg/mL), or a combination of Bjssu + Mn² + or Bjssu + Mn² + /HA. Cultures were assessed at intervals of 1, 2, 3, 6, 12, and 24 hours. Additionally, a morphological analysis was performed using rapid Panoptic staining to identify cellular damage caused by venom cytotoxicity. The results showed that Mn² + at concentrations of 1 and 2 mM, whether combined with HA or not, provided significant protection against venom cytotoxicity (1.25 and 2.5 µg/mL), especially during the initial hours of exposure. This was confirmed by rapid Panoptic staining, demonstrating significant preservation of cellular integrity with Mn² + /HA treatment. The combination of Mn² + and HA appears to be a promising approach to mitigate local effects, suggesting a potential complementary therapeutic tool to antivenom therapy