Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de
|
Departamento: |
Faculdade de Medicina
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/6277 |
Resumo: | Objectives: To evaluate the characteristics of cell nuclei of melanocytes using digital nuclear morphometric analysis. To evaluate the nuclear morphometric differences between nevi excised in winter or summer; demonstrating the presence of characteristics and irregularities in nuclear morphology caused by solar radiation on nevi. To consider whether there are morphological differences between common acquired nevi, dysplastic nevi and melanomas. To access the potential use of nuclear morphometry to detect cell senescence, apoptosis and other nuclear irregularities of nevi and melanomas in HE stained slides of paraffin embedded specimens. Methodology: Histopathological specimens were subjected to digital image analysis. Results: Through this pilot study, glass slides obtained from biopsies of 30 patients were analyzed; 10 nevi excised in winter, 5 nevi excised in summer, 9 dysplastic nevi and 6 melanomas. Between winter and summer nevi; secondary variables able to perform such a separation were 'variability Area' and % of nuclei with NII higher than 1SD. Conclusion: There are differences of nuclear morphometric characteristics of excised nevi in winter and summer and can be caused by cumulative effects of solar radiation on nevi. There are morphometric differences between common acquired nevi, dysplastic nevi and melanomas. The dysplastic nevi and melanomas were separated by the average area and percentage of nuclei with area above one standard deviation of the mean area. |
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Silva, Vinicius Duval da562.260.800-78http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765156H1971.872.160-68http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4256016E5G?tze, Fernanda Mendes2015-08-27T11:35:28Z2015-07-10http://tede2.pucrs.br/tede2/handle/tede/6277Objectives: To evaluate the characteristics of cell nuclei of melanocytes using digital nuclear morphometric analysis. To evaluate the nuclear morphometric differences between nevi excised in winter or summer; demonstrating the presence of characteristics and irregularities in nuclear morphology caused by solar radiation on nevi. To consider whether there are morphological differences between common acquired nevi, dysplastic nevi and melanomas. To access the potential use of nuclear morphometry to detect cell senescence, apoptosis and other nuclear irregularities of nevi and melanomas in HE stained slides of paraffin embedded specimens. Methodology: Histopathological specimens were subjected to digital image analysis. Results: Through this pilot study, glass slides obtained from biopsies of 30 patients were analyzed; 10 nevi excised in winter, 5 nevi excised in summer, 9 dysplastic nevi and 6 melanomas. Between winter and summer nevi; secondary variables able to perform such a separation were 'variability Area' and % of nuclei with NII higher than 1SD. Conclusion: There are differences of nuclear morphometric characteristics of excised nevi in winter and summer and can be caused by cumulative effects of solar radiation on nevi. There are morphometric differences between common acquired nevi, dysplastic nevi and melanomas. The dysplastic nevi and melanomas were separated by the average area and percentage of nuclei with area above one standard deviation of the mean area.Objetivos: Avaliar as caracter?sticas dos n?cleos celulares de melan?citos atrav?s de an?lise morfom?trica nuclear digital e as diferen?as morfom?tricas nucleares entre nevos excisados no inverno e nevos excisados ap?s o ver?o; para detectar poss?veis caracter?sticas e irregularidades na morfometria nuclear provocadas pela radia??o solar sobre nevos. Averiguar a possibilidade de identifica??o de diferen?as morfom?tricas entre nevos adquiridos comuns, nevos displ?sicos e melanomas e avaliar a possibilidade de detec??o de senesc?ncia, apoptose e outras irregularidades nucleares em nevos e melanomas por morfometria digital em cortes histol?gicos corado por HE a partir de blocos de parafina. Metodologia: Os esp?cimes histopatol?gicos foram submetidos ? an?lise de imagem digital realizada atrav?s de ferramentas de avalia??o nuclear digital. Resultados: Neste estudo piloto foram analisados os esp?cimes de bi?psia de 30 pacientes, compostos por: 10 nevos biopsiados no inverno, 5 nevos no ver?o, 9 nevos displ?sicos e 6 melanomas malignos. Nos nevos de inverno e nevos de ver?o; as vari?veis capazes de realizar tal separa??o foram ?Variabilidade da ?rea? e ?porcentagem de n?cleos com NII acima do valor da m?dia+1DP. Conclus?o: Foram detectadas diferen?as morfom?tricas nucleares entre nevos excisados no inverno e nevos excisados ap?s o ver?o, que podem ser provocadas pelos efeitos cumulativos da radia??o solar sobre nevos. Foram catacterizadas diferen?as morfom?tricas entre nevos adquiridos comuns, nevos displ?sicos e melanomas. Os nevos displ?sicos e melanomas foram separados pelas caracter?sticas de ?rea m?dia e porcentagem de n?cleos com ?rea acima de um desvio padr?o da m?dia.Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-08-27T11:35:28Z No. of bitstreams: 1 474248 - Texto Completo.pdf: 1288519 bytes, checksum: 76a0f632ccb7c577dfc183ba0e91a44b (MD5)Made available in DSpace on 2015-08-27T11:35:28Z (GMT). No. of bitstreams: 1 474248 - Texto Completo.pdf: 1288519 bytes, checksum: 76a0f632ccb7c577dfc183ba0e91a44b (MD5) Previous issue date: 2015-07-10application/pdfporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Medicina e Ci?ncias da Sa?dePUCRSBrasilFaculdade de MedicinaMEDICINADERMATOLOGIANEVOSMELANOMACIENCIAS DA SAUDE::MEDICINAAvalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digitalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7620745074616285884600600600-8624664729441623247-969369452308786627info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAILTEXTLICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/6277/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/62772015-09-21 08:32:34.497oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-09-21T11:32:34Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
title |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
spellingShingle |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital G?tze, Fernanda Mendes MEDICINA DERMATOLOGIA NEVOS MELANOMA CIENCIAS DA SAUDE::MEDICINA |
title_short |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
title_full |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
title_fullStr |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
title_full_unstemmed |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
title_sort |
Avalia??o de nevos cut?neos e melanomas por ferramenta de an?lise morfom?trica nuclear digital |
author |
G?tze, Fernanda Mendes |
author_facet |
G?tze, Fernanda Mendes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Vinicius Duval da |
dc.contributor.advisor1ID.fl_str_mv |
562.260.800-78 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765156H1 |
dc.contributor.authorID.fl_str_mv |
971.872.160-68 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4256016E5 |
dc.contributor.author.fl_str_mv |
G?tze, Fernanda Mendes |
contributor_str_mv |
Silva, Vinicius Duval da |
dc.subject.por.fl_str_mv |
MEDICINA DERMATOLOGIA NEVOS MELANOMA |
topic |
MEDICINA DERMATOLOGIA NEVOS MELANOMA CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Objectives: To evaluate the characteristics of cell nuclei of melanocytes using digital nuclear morphometric analysis. To evaluate the nuclear morphometric differences between nevi excised in winter or summer; demonstrating the presence of characteristics and irregularities in nuclear morphology caused by solar radiation on nevi. To consider whether there are morphological differences between common acquired nevi, dysplastic nevi and melanomas. To access the potential use of nuclear morphometry to detect cell senescence, apoptosis and other nuclear irregularities of nevi and melanomas in HE stained slides of paraffin embedded specimens. Methodology: Histopathological specimens were subjected to digital image analysis. Results: Through this pilot study, glass slides obtained from biopsies of 30 patients were analyzed; 10 nevi excised in winter, 5 nevi excised in summer, 9 dysplastic nevi and 6 melanomas. Between winter and summer nevi; secondary variables able to perform such a separation were 'variability Area' and % of nuclei with NII higher than 1SD. Conclusion: There are differences of nuclear morphometric characteristics of excised nevi in winter and summer and can be caused by cumulative effects of solar radiation on nevi. There are morphometric differences between common acquired nevi, dysplastic nevi and melanomas. The dysplastic nevi and melanomas were separated by the average area and percentage of nuclei with area above one standard deviation of the mean area. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-08-27T11:35:28Z |
dc.date.issued.fl_str_mv |
2015-07-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://tede2.pucrs.br/tede2/handle/tede/6277 |
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http://tede2.pucrs.br/tede2/handle/tede/6277 |
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por |
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por |
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7620745074616285884 |
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600 600 600 |
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-969369452308786627 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de |
dc.publisher.initials.fl_str_mv |
PUCRS |
dc.publisher.country.fl_str_mv |
Brasil |
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Faculdade de Medicina |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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