Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina
Ano de defesa: | 2016 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais
|
Departamento: |
Faculdade de Engenharia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/6953 |
Resumo: | Chemotherapy is highlighted for the treatment of many classes of tumors, especially by new drug delivery nanosystems. The drug delivery nanosystems are focused on greater selectivity for abnormal tissue, decrease adverse effects, dose reduction and decreased frequency applications. Due to the scale of these systems, the EPR effect favors the permeabilization in blood vessels and retention in tumor tissue particles. This thesis performed vinblastine extractions from C. roseus using water and ethanol as co-solvents for CO2 at 300 bar and at different temperatures, those experiments showed the viability of this extraction method, subsequent extractions were optimized using ethanol. The results are compared to traditional extraction methods such as solid-liquid extraction the results found was up to 92.41%. Concluding that vinblastine extraction using carbon dioxide and ethanol mixtures at high pressure is possible. The PLGA nanoparticles containing vinblastine showed again the reproducibility of the nanoemulsion method of ensuring the production of homogeneous nanoparticles with and without drug and cell viability experiments show that the three formulations of PLGA nanoparticles containing vinblastine potentiated drug activity at the lower doses, 1 and 5 ?g/mL, compared to free drug. The studies of glycopolymers synthesis presented the novel synthesis of an ATRP initiator based on PLGA without using PEG spacers. This initiator may, in future studies, be used for modifying PLGA. Successive attempts monomer polymerization saccharide via ATRP in water and DMSO leads to the conclusion that this approach is not the most suitable for the syntheses of glycopolymers graft from a PLGA initiator without the use of PEG spacers. |
id |
P_RS_821c6d4f6d5af658c452eb232fac7641 |
---|---|
oai_identifier_str |
oai:tede2.pucrs.br:tede/6953 |
network_acronym_str |
P_RS |
network_name_str |
Biblioteca Digital de Teses e Dissertações da PUC_RS |
repository_id_str |
|
spelling |
Cassel, Eduardo436.316.060-87http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723188A7017.574.260-00http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4481919J1Falc?o, Manuel Alves2016-09-12T16:18:06Z2016-03-30http://tede2.pucrs.br/tede2/handle/tede/6953Chemotherapy is highlighted for the treatment of many classes of tumors, especially by new drug delivery nanosystems. The drug delivery nanosystems are focused on greater selectivity for abnormal tissue, decrease adverse effects, dose reduction and decreased frequency applications. Due to the scale of these systems, the EPR effect favors the permeabilization in blood vessels and retention in tumor tissue particles. This thesis performed vinblastine extractions from C. roseus using water and ethanol as co-solvents for CO2 at 300 bar and at different temperatures, those experiments showed the viability of this extraction method, subsequent extractions were optimized using ethanol. The results are compared to traditional extraction methods such as solid-liquid extraction the results found was up to 92.41%. Concluding that vinblastine extraction using carbon dioxide and ethanol mixtures at high pressure is possible. The PLGA nanoparticles containing vinblastine showed again the reproducibility of the nanoemulsion method of ensuring the production of homogeneous nanoparticles with and without drug and cell viability experiments show that the three formulations of PLGA nanoparticles containing vinblastine potentiated drug activity at the lower doses, 1 and 5 ?g/mL, compared to free drug. The studies of glycopolymers synthesis presented the novel synthesis of an ATRP initiator based on PLGA without using PEG spacers. This initiator may, in future studies, be used for modifying PLGA. Successive attempts monomer polymerization saccharide via ATRP in water and DMSO leads to the conclusion that this approach is not the most suitable for the syntheses of glycopolymers graft from a PLGA initiator without the use of PEG spacers.A quimioterapia ? op??o terap?utica para o tratamento de diversas classes de tumores, principalmente pelos novos nanosistemas de libera??o modificada de f?rmacos. Os nanosistemas de libera??o modificada t?m como foco a maior seletividade aos tecidos anormais, a diminui??o de efeitos adversos, redu??o da dose e diminui??o da frequ?ncia de aplica??es. Devido a escala destes sistemas, o efeito EPR favorece a permeabiliza??o nos vasos sangu?neos e a reten??o de part?culas no tecido tumoral. Nesta tese foram realizadas extra??es de vimblastina a partir de C. roseus utilizando ?gua e etanol como cossolventes de CO2 a 300 bar e a diferentes temperaturas mostraram a viabilidade do m?todo de extra??o, posteriormente, as extra??es foram otimizadas utilizando etanol. Quando os resultados encontrados s?o comparados a m?todos tradicionais de extra??o como a extra??o s?lido-l?quido o resultado encontrado foi de at? 92,41% superior. Concluindo, que a extra??o de vimblastina utilizando misturas de di?xido de carbono e etanol a altas press?es ? poss?vel. As nanopart?culas de PLGA contendo vimblastina demonstraram a reprodutibilidade do m?todo de nanoemuls?o garantindo a produ??o de nanopart?culas homog?neas, com e sem f?rmaco. Os experimentos de viabilidade celular mostraram que as tr?s formula??es de nanopart?culas de PLGA contendo vimblastina potencializaram a atividade do f?rmaco para as doses mais baixas, 1 e 5 ?g/mL, em rela??o ao f?rmaco livre. Os estudos realizados de s?ntese de glicopol?meros apresentaram a s?ntese in?dita de um iniciador de ATRP baseado em PLGA sem o uso de espa?adores de PEG. Este iniciador pode, em futuros estudos, ser utilizado para a modifica??o de PLGA. As sucessivas tentativas de polimeriza??o de mon?meros de sacar?deos via ATRP em ?gua e DMSO leva a conclus?o que esta metodologia n?o ? a mais adequada para s?nteses glicopol?meros conjugados a PLGA sem o uso de espa?adores de PEG.Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-09-12T16:18:06Z No. of bitstreams: 1 TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf: 3543313 bytes, checksum: c6209bccf7d4cd318c874850a1bc853d (MD5)Made available in DSpace on 2016-09-12T16:18:06Z (GMT). No. of bitstreams: 1 TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf: 3543313 bytes, checksum: c6209bccf7d4cd318c874850a1bc853d (MD5) Previous issue date: 2016-03-30application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/166240/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Engenharia e Tecnologia de MateriaisPUCRSBrasilFaculdade de EngenhariaEXTRA??O SUPERCR?TICADI?XIDO DE CARBONONANOPART?CULASENGENHARIA QU?MICAENGENHARIA DE MATERIAISENGENHARIASEstudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastinaStudy of C. roseus extraction and production of nanoparticles for vinblastine releaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-7432719344215120122600600600-6557705727614397854518971056484826825info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAILTES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.jpgTES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.jpgimage/jpeg4166http://tede2.pucrs.br/tede2/bitstream/tede/6953/5/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.jpgc1d2495eb25f505a4231621d37204cd7MD55TEXTTES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.txtTES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.txttext/plain142477http://tede2.pucrs.br/tede2/bitstream/tede/6953/4/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.txt011de1df920607ca183b5d274895b2caMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/6953/3/license.txt5a9d6006225b368ef605ba16b4f6d1beMD53ORIGINALTES_MANUEL_ALVES_FALCAO_COMPLETO.pdfTES_MANUEL_ALVES_FALCAO_COMPLETO.pdfapplication/pdf3543313http://tede2.pucrs.br/tede2/bitstream/tede/6953/2/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdfc6209bccf7d4cd318c874850a1bc853dMD52tede/69532016-09-12 20:00:37.514oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2016-09-12T23:00:37Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
dc.title.alternative.eng.fl_str_mv |
Study of C. roseus extraction and production of nanoparticles for vinblastine release |
title |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
spellingShingle |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina Falc?o, Manuel Alves EXTRA??O SUPERCR?TICA DI?XIDO DE CARBONO NANOPART?CULAS ENGENHARIA QU?MICA ENGENHARIA DE MATERIAIS ENGENHARIAS |
title_short |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
title_full |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
title_fullStr |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
title_full_unstemmed |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
title_sort |
Estudo de extra??o de C. roseus e produ??o de nanopart?culas para libera??o de vimblastina |
author |
Falc?o, Manuel Alves |
author_facet |
Falc?o, Manuel Alves |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cassel, Eduardo |
dc.contributor.advisor1ID.fl_str_mv |
436.316.060-87 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723188A7 |
dc.contributor.authorID.fl_str_mv |
017.574.260-00 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4481919J1 |
dc.contributor.author.fl_str_mv |
Falc?o, Manuel Alves |
contributor_str_mv |
Cassel, Eduardo |
dc.subject.por.fl_str_mv |
EXTRA??O SUPERCR?TICA DI?XIDO DE CARBONO NANOPART?CULAS ENGENHARIA QU?MICA ENGENHARIA DE MATERIAIS |
topic |
EXTRA??O SUPERCR?TICA DI?XIDO DE CARBONO NANOPART?CULAS ENGENHARIA QU?MICA ENGENHARIA DE MATERIAIS ENGENHARIAS |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS |
description |
Chemotherapy is highlighted for the treatment of many classes of tumors, especially by new drug delivery nanosystems. The drug delivery nanosystems are focused on greater selectivity for abnormal tissue, decrease adverse effects, dose reduction and decreased frequency applications. Due to the scale of these systems, the EPR effect favors the permeabilization in blood vessels and retention in tumor tissue particles. This thesis performed vinblastine extractions from C. roseus using water and ethanol as co-solvents for CO2 at 300 bar and at different temperatures, those experiments showed the viability of this extraction method, subsequent extractions were optimized using ethanol. The results are compared to traditional extraction methods such as solid-liquid extraction the results found was up to 92.41%. Concluding that vinblastine extraction using carbon dioxide and ethanol mixtures at high pressure is possible. The PLGA nanoparticles containing vinblastine showed again the reproducibility of the nanoemulsion method of ensuring the production of homogeneous nanoparticles with and without drug and cell viability experiments show that the three formulations of PLGA nanoparticles containing vinblastine potentiated drug activity at the lower doses, 1 and 5 ?g/mL, compared to free drug. The studies of glycopolymers synthesis presented the novel synthesis of an ATRP initiator based on PLGA without using PEG spacers. This initiator may, in future studies, be used for modifying PLGA. Successive attempts monomer polymerization saccharide via ATRP in water and DMSO leads to the conclusion that this approach is not the most suitable for the syntheses of glycopolymers graft from a PLGA initiator without the use of PEG spacers. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-09-12T16:18:06Z |
dc.date.issued.fl_str_mv |
2016-03-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/6953 |
url |
http://tede2.pucrs.br/tede2/handle/tede/6953 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-7432719344215120122 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-655770572761439785 |
dc.relation.cnpq.fl_str_mv |
4518971056484826825 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais |
dc.publisher.initials.fl_str_mv |
PUCRS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Engenharia |
publisher.none.fl_str_mv |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da PUC_RS |
collection |
Biblioteca Digital de Teses e Dissertações da PUC_RS |
bitstream.url.fl_str_mv |
http://tede2.pucrs.br/tede2/bitstream/tede/6953/5/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.jpg http://tede2.pucrs.br/tede2/bitstream/tede/6953/4/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf.txt http://tede2.pucrs.br/tede2/bitstream/tede/6953/3/license.txt http://tede2.pucrs.br/tede2/bitstream/tede/6953/2/TES_MANUEL_ALVES_FALCAO_COMPLETO.pdf |
bitstream.checksum.fl_str_mv |
c1d2495eb25f505a4231621d37204cd7 011de1df920607ca183b5d274895b2ca 5a9d6006225b368ef605ba16b4f6d1be c6209bccf7d4cd318c874850a1bc853d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
biblioteca.central@pucrs.br|| |
_version_ |
1796793222516703232 |