Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau
Ano de defesa: | 2016 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de
|
Departamento: |
Faculdade de Medicina
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/7035 |
Resumo: | Objective: To evaluate association between histological grade of low (CIN I) and high-grade intraepithelial cervical lesions (CIN II and III) and the immunohistochemical expression of p16INK4a, hTert and ki67 in order to establish the behavior of these lesions and recurrence during follow-up of two years. Patients and Methods: a historical cohort study of 94 women to analyze 3 groups of patients with low (CIN I) and high-grade (CIN II and III) cervical intraepithelial lesions underwent to knife, to determine the recurrence of disease associated to expression of immunohistochemical markers p16INK4a, Ki67 and hTERT. Results: Mean age of the patients was 38.2 years. Patients with CIN I, there was a lower p16INK4a expression, while in patients CIN II or I / II a higher frequency of p16INK4a ?10% was observed. In patients with CIN III there was a higher frequency of p16INK4a expression (> 50%). There were more patients with Ki67 ?10% of the cell population and lower frequency of Ki67 expression (> 50%) in CIN I. In CIN III there were fewer patients in the category Ki67 ?10% and CIN II and III group there were more patients with absent expression of Ki67. There wasn't association between hTert and histological grade. When comparing the markers between subjects with and without recurrence there was no statistically significant difference. Conclusion: There was a statistically significant association between p16INK4a, P16iNK4aM and Ki67 and histological grade, however there was no statistical difference in the expression of these markers in relation to recurrence of disease during the study period. |
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Silva, Vinicius Duval da562.260.800-78http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765156H1Gon?alves, Manoel Afonso Guimar?eshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781128A0007.996.390-01http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4550349U4Goulart, Ana Paula Szezepaniak2016-11-16T13:11:08Z2016-08-26http://tede2.pucrs.br/tede2/handle/tede/7035Objective: To evaluate association between histological grade of low (CIN I) and high-grade intraepithelial cervical lesions (CIN II and III) and the immunohistochemical expression of p16INK4a, hTert and ki67 in order to establish the behavior of these lesions and recurrence during follow-up of two years. Patients and Methods: a historical cohort study of 94 women to analyze 3 groups of patients with low (CIN I) and high-grade (CIN II and III) cervical intraepithelial lesions underwent to knife, to determine the recurrence of disease associated to expression of immunohistochemical markers p16INK4a, Ki67 and hTERT. Results: Mean age of the patients was 38.2 years. Patients with CIN I, there was a lower p16INK4a expression, while in patients CIN II or I / II a higher frequency of p16INK4a ?10% was observed. In patients with CIN III there was a higher frequency of p16INK4a expression (> 50%). There were more patients with Ki67 ?10% of the cell population and lower frequency of Ki67 expression (> 50%) in CIN I. In CIN III there were fewer patients in the category Ki67 ?10% and CIN II and III group there were more patients with absent expression of Ki67. There wasn't association between hTert and histological grade. When comparing the markers between subjects with and without recurrence there was no statistically significant difference. Conclusion: There was a statistically significant association between p16INK4a, P16iNK4aM and Ki67 and histological grade, however there was no statistical difference in the expression of these markers in relation to recurrence of disease during the study period.Objetivo: Avaliar a associa??o entre gradua??o histol?gica e a express?o imunoistoqu?mica para P16iNK4a, hTert e ki67, a fim de estabelecer o comportamento dessas les?es quanto ? recorr?ncia durante o seguimento de dois anos. Pacientes e M?todos: Estudo de coorte hist?rica incluindo 94 mulheres, em que foram analisados 3 grupos de pacientes com les?es intraepiteliais cervicais de baixo (NIC I) e alto grau , classificadas em NIC II e III, submetidas ? coniza??o, a fim de determinar a recorr?ncia da doen?a conforme a express?o dos marcadores imunoistoqu?micos P16iNK4a, Ki67 e hTert. Resultados: A idade m?dia das pacientes foi de 38,2 anos. Nas pacientes com NIC I, houve maior frequ?ncia de P16iNK4a ausente, nas pacientes NIC II ou I/II observou-se maior frequ?ncia com P16iNK4a ?10%. Nas pacientes NIC III observou-se maior frequ?ncia de express?o de P16iNK4a (>50%). Na categoria NIC I houve mais pacientes com Ki67 ?10% e menor frequ?ncia de Ki67 (>50%). Na categoria NIC III houve menos pacientes na categoria Ki67 ?10% e no grupo NIC II e III houve mais pacientes com Ki67 ausente. N?o houve associa??o entre a express?o do marcador imunoistoqu?mico hTert e gradua??o histol?gica. Quando comparadas as express?es dos marcadores entre sujeitos com e sem recorr?ncia, n?o houve diferen?as estatisticamente significativas. Conclus?o: Houve uma associa??o estatisticamente significativa entre P16iNK4a, P16iNK4aM e Ki67 e a gradua??o histol?gica, entretanto n?o houve diferen?a estat?stica na express?o desses marcadores em rela??o ? recorr?ncia da doen?a no per?odo avaliado.Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-11-16T13:11:07Z No. of bitstreams: 1 DIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf: 1331186 bytes, checksum: c62191ee8989facb5039525bce1735d3 (MD5)Made available in DSpace on 2016-11-16T13:11:08Z (GMT). No. of bitstreams: 1 DIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf: 1331186 bytes, checksum: c62191ee8989facb5039525bce1735d3 (MD5) Previous issue date: 2016-08-26application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/166709/DIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Medicina e Ci?ncias da Sa?dePUCRSBrasilFaculdade de MedicinaNEOPLASIA INTRA-EPITELIAL CERVICALIMUNOISTOQU?MICANEOPLASIAS DOS GENITAIS FEMININOSGINECOLOGIAMEDICINACIENCIAS DA SAUDE::MEDICINAAvalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grauinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7620745074616285884600600600-8624664729441623247-969369452308786627info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAILDIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.jpgDIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.jpgimage/jpeg3259http://tede2.pucrs.br/tede2/bitstream/tede/7035/4/DIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.jpgf82055d497d9d0bcedaa4885d5e411a2MD54TEXTDIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.txtDIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.txttext/plain111655http://tede2.pucrs.br/tede2/bitstream/tede/7035/5/DIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdf.txt89067936803cfcf1d5699a24f96a325cMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/7035/3/license.txt5a9d6006225b368ef605ba16b4f6d1beMD53ORIGINALDIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdfDIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdfapplication/pdf1331186http://tede2.pucrs.br/tede2/bitstream/tede/7035/2/DIS_ANA_PAULA_SZEZEPANIAK_GOULART_COMPLETO.pdfc62191ee8989facb5039525bce1735d3MD52tede/70352016-11-16 20:00:34.122oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2016-11-16T22:00:34Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
title |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
spellingShingle |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau Goulart, Ana Paula Szezepaniak NEOPLASIA INTRA-EPITELIAL CERVICAL IMUNOISTOQU?MICA NEOPLASIAS DOS GENITAIS FEMININOS GINECOLOGIA MEDICINA CIENCIAS DA SAUDE::MEDICINA |
title_short |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
title_full |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
title_fullStr |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
title_full_unstemmed |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
title_sort |
Avalia??o da express?o de telomerase (hTert), Ki-67 e P16iNK4a em les?es intraepiteliais cervicais de baixo e alto grau |
author |
Goulart, Ana Paula Szezepaniak |
author_facet |
Goulart, Ana Paula Szezepaniak |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Vinicius Duval da |
dc.contributor.advisor1ID.fl_str_mv |
562.260.800-78 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765156H1 |
dc.contributor.advisor-co1.fl_str_mv |
Gon?alves, Manoel Afonso Guimar?es |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781128A0 |
dc.contributor.authorID.fl_str_mv |
007.996.390-01 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4550349U4 |
dc.contributor.author.fl_str_mv |
Goulart, Ana Paula Szezepaniak |
contributor_str_mv |
Silva, Vinicius Duval da Gon?alves, Manoel Afonso Guimar?es |
dc.subject.por.fl_str_mv |
NEOPLASIA INTRA-EPITELIAL CERVICAL IMUNOISTOQU?MICA NEOPLASIAS DOS GENITAIS FEMININOS GINECOLOGIA MEDICINA |
topic |
NEOPLASIA INTRA-EPITELIAL CERVICAL IMUNOISTOQU?MICA NEOPLASIAS DOS GENITAIS FEMININOS GINECOLOGIA MEDICINA CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Objective: To evaluate association between histological grade of low (CIN I) and high-grade intraepithelial cervical lesions (CIN II and III) and the immunohistochemical expression of p16INK4a, hTert and ki67 in order to establish the behavior of these lesions and recurrence during follow-up of two years. Patients and Methods: a historical cohort study of 94 women to analyze 3 groups of patients with low (CIN I) and high-grade (CIN II and III) cervical intraepithelial lesions underwent to knife, to determine the recurrence of disease associated to expression of immunohistochemical markers p16INK4a, Ki67 and hTERT. Results: Mean age of the patients was 38.2 years. Patients with CIN I, there was a lower p16INK4a expression, while in patients CIN II or I / II a higher frequency of p16INK4a ?10% was observed. In patients with CIN III there was a higher frequency of p16INK4a expression (> 50%). There were more patients with Ki67 ?10% of the cell population and lower frequency of Ki67 expression (> 50%) in CIN I. In CIN III there were fewer patients in the category Ki67 ?10% and CIN II and III group there were more patients with absent expression of Ki67. There wasn't association between hTert and histological grade. When comparing the markers between subjects with and without recurrence there was no statistically significant difference. Conclusion: There was a statistically significant association between p16INK4a, P16iNK4aM and Ki67 and histological grade, however there was no statistical difference in the expression of these markers in relation to recurrence of disease during the study period. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-11-16T13:11:08Z |
dc.date.issued.fl_str_mv |
2016-08-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://tede2.pucrs.br/tede2/handle/tede/7035 |
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http://tede2.pucrs.br/tede2/handle/tede/7035 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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7620745074616285884 |
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600 600 600 |
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-8624664729441623247 |
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-969369452308786627 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de |
dc.publisher.initials.fl_str_mv |
PUCRS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Medicina |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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