Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais
|
Departamento: |
Faculdade de Engenharia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/5945 |
Resumo: | The Baccharis genus has more than 500 species discribed and 90% occur in South America. It has highlighted important feature in the folk medicine being used for digestive problems. Studies report the presence of phenolic compounds and phenolic acids in its non-volatile extracts. Non-volatile extracts are obtained by infusion and/or maceration of parts of the plant in solvent. One way to obtain pure extracts without the need of evaporation of the solvent is the extraction by supercritical fluid. The aim of the present study was to obtain non-volatile compounds of seven Baccharis species: Baccharis microdonta, B. megapotamica, B. vulneraria, B. usteri, B. trimera, B. tridentata, B. uncinella by supercritical extraction in different conditions of operation, analyze them in different chromatographic methods and test its biological activities such as antioxidant and antimicrobial. As it shows high antioxidant potencial and high availability in the nature, the B. uncinella species were selected to continue our studies in order to perform the supercritical impregnation of the extract in sutures, one polymer matrix of PLGA. The antioxidant activity was the chosen characteristic to aggregate in the suture with the objective to enhance the healing were it is used. The results obtained shows that the impregnated material was not visualized through image analysis, but quantification and liberation tests as well as antioxidant activity shows that the impregnation has occurred. The impregnation condition at 150 bar showed superior results in relation to the impregnation at 200 bar, about 50% more mass impregnated and a 30% higher antioxidant activity. |
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Cassel, Eduardo436.316.060-87http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723188A7805.466.330-72http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4711892A3Lucas, Aline Machado2015-04-24T14:07:47Z2015-03-25http://tede2.pucrs.br/tede2/handle/tede/5945The Baccharis genus has more than 500 species discribed and 90% occur in South America. It has highlighted important feature in the folk medicine being used for digestive problems. Studies report the presence of phenolic compounds and phenolic acids in its non-volatile extracts. Non-volatile extracts are obtained by infusion and/or maceration of parts of the plant in solvent. One way to obtain pure extracts without the need of evaporation of the solvent is the extraction by supercritical fluid. The aim of the present study was to obtain non-volatile compounds of seven Baccharis species: Baccharis microdonta, B. megapotamica, B. vulneraria, B. usteri, B. trimera, B. tridentata, B. uncinella by supercritical extraction in different conditions of operation, analyze them in different chromatographic methods and test its biological activities such as antioxidant and antimicrobial. As it shows high antioxidant potencial and high availability in the nature, the B. uncinella species were selected to continue our studies in order to perform the supercritical impregnation of the extract in sutures, one polymer matrix of PLGA. The antioxidant activity was the chosen characteristic to aggregate in the suture with the objective to enhance the healing were it is used. The results obtained shows that the impregnated material was not visualized through image analysis, but quantification and liberation tests as well as antioxidant activity shows that the impregnation has occurred. The impregnation condition at 150 bar showed superior results in relation to the impregnation at 200 bar, about 50% more mass impregnated and a 30% higher antioxidant activity.O g?nero Baccharis possui mais de 500 esp?cies descritas sendo que 90% destas ocorrem na Am?rica do Sul. Possui importante destaque na medicina popular sendo utilizadas para problemas digestivos. Estudos reportam a presen?a de compostos fen?licos como flavonoides e ?cidos fen?licos em seus extratos n?o vol?teis. Extratos n?o vol?teis normalmente s?o obtidos por infus?o e/ou macera??o de partes de planta em solvente. Uma forma de obten??o de extratos puros sem necessidade de evapora??o do solvente ? a extra??o por fluido supercr?tico. O objetivo do presente estudo foi obter compostos n?o vol?teis de sete esp?cies de Baccharis: Baccharis microdonta, B. megapotamica, B. vulneraria, B. usteri, B. trimera, B. tridentata, B. uncinella por extra??o supercr?tica em diferentes condi??es de opera??o, analis?-los por diferentes m?todos cromatogr?ficos e testar suas atividades biol?gicas tais como atividade antioxidante e antimicrobiana. Por apresentar um potencial antioxidante elevado e grande disponibilidade na natureza, a esp?cie B. uncinella foi selecionada para dar continuidade aos estudos e realizar a impregna??o supercr?tica do extrato selecionado em fios de sutura, uma matriz polim?rica de PLGA. A atividade antioxidante foi a caracter?stica escolhida para agregar ao fio de sutura com o objetivo de potencializar a cicatriza??o do local onde for aplicado. Os resultados obtidos mostram que o material impregnado n?o foi visualizado atrav?s da an?lise de imagens, mas testes de quantifica??o e libera??o, assim como de atividade antioxidante, comprovaram que ocorre a impregna??o do extrato. A condi??o de impregna??o a 150 bar apresentou resultados superiores em rela??o a impregna??o a 200 bar, aproximadamente 50% mais massa impregnada e e uma atividade antioxidante 30% maior.Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-04-24T14:07:47Z No. of bitstreams: 1 467627 - Texto Completo.pdf: 2210744 bytes, checksum: aad8668a5522012bd1f3603c51df5d00 (MD5)Made available in DSpace on 2015-04-24T14:07:47Z (GMT). No. of bitstreams: 1 467627 - Texto Completo.pdf: 2210744 bytes, checksum: aad8668a5522012bd1f3603c51df5d00 (MD5) Previous issue date: 2015-03-25Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/17776/467627%20-%20Texto%20Completo.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Engenharia e Tecnologia de MateriaisPUCRSBrasilFaculdade de EngenhariaENGENHARIA DE MATERIAISENGENHARIA QU?MICAEXTRA??O SUPERCR?TICAASTER?CEAS?LEOS ESSENCIAISPLANTAS AROM?TICASENGENHARIASEstudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-7432719344215120122600600600600-65577057276143978545189710564848268252075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL467627 - Texto Completo.pdf.jpg467627 - Texto Completo.pdf.jpgimage/jpeg4582http://tede2.pucrs.br/tede2/bitstream/tede/5945/4/467627+-+Texto+Completo.pdf.jpg364b5b038bc1825950142902166a87afMD54TEXT467627 - Texto Completo.pdf.txt467627 - Texto Completo.pdf.txttext/plain149653http://tede2.pucrs.br/tede2/bitstream/tede/5945/3/467627+-+Texto+Completo.pdf.txtdcc3f9dad13d54c4456c547d8823fb9cMD53ORIGINAL467627 - Texto Completo.pdf467627 - Texto Completo.pdfapplication/pdf2210744http://tede2.pucrs.br/tede2/bitstream/tede/5945/2/467627+-+Texto+Completo.pdfaad8668a5522012bd1f3603c51df5d00MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/5945/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/59452015-04-25 04:00:25.119oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-04-25T07:00:25Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
title |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
spellingShingle |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis Lucas, Aline Machado ENGENHARIA DE MATERIAIS ENGENHARIA QU?MICA EXTRA??O SUPERCR?TICA ASTER?CEAS ?LEOS ESSENCIAIS PLANTAS AROM?TICAS ENGENHARIAS |
title_short |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
title_full |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
title_fullStr |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
title_full_unstemmed |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
title_sort |
Estudos sistem?ticos de obten??o e impregna??o supercr?tica de extratos de baccharis |
author |
Lucas, Aline Machado |
author_facet |
Lucas, Aline Machado |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cassel, Eduardo |
dc.contributor.advisor1ID.fl_str_mv |
436.316.060-87 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723188A7 |
dc.contributor.authorID.fl_str_mv |
805.466.330-72 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4711892A3 |
dc.contributor.author.fl_str_mv |
Lucas, Aline Machado |
contributor_str_mv |
Cassel, Eduardo |
dc.subject.por.fl_str_mv |
ENGENHARIA DE MATERIAIS ENGENHARIA QU?MICA EXTRA??O SUPERCR?TICA ASTER?CEAS ?LEOS ESSENCIAIS PLANTAS AROM?TICAS |
topic |
ENGENHARIA DE MATERIAIS ENGENHARIA QU?MICA EXTRA??O SUPERCR?TICA ASTER?CEAS ?LEOS ESSENCIAIS PLANTAS AROM?TICAS ENGENHARIAS |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS |
description |
The Baccharis genus has more than 500 species discribed and 90% occur in South America. It has highlighted important feature in the folk medicine being used for digestive problems. Studies report the presence of phenolic compounds and phenolic acids in its non-volatile extracts. Non-volatile extracts are obtained by infusion and/or maceration of parts of the plant in solvent. One way to obtain pure extracts without the need of evaporation of the solvent is the extraction by supercritical fluid. The aim of the present study was to obtain non-volatile compounds of seven Baccharis species: Baccharis microdonta, B. megapotamica, B. vulneraria, B. usteri, B. trimera, B. tridentata, B. uncinella by supercritical extraction in different conditions of operation, analyze them in different chromatographic methods and test its biological activities such as antioxidant and antimicrobial. As it shows high antioxidant potencial and high availability in the nature, the B. uncinella species were selected to continue our studies in order to perform the supercritical impregnation of the extract in sutures, one polymer matrix of PLGA. The antioxidant activity was the chosen characteristic to aggregate in the suture with the objective to enhance the healing were it is used. The results obtained shows that the impregnated material was not visualized through image analysis, but quantification and liberation tests as well as antioxidant activity shows that the impregnation has occurred. The impregnation condition at 150 bar showed superior results in relation to the impregnation at 200 bar, about 50% more mass impregnated and a 30% higher antioxidant activity. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-04-24T14:07:47Z |
dc.date.issued.fl_str_mv |
2015-03-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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http://tede2.pucrs.br/tede2/handle/tede/5945 |
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por |
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por |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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