Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos
Ano de defesa: | 2019 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais
|
Departamento: |
Escola Polit?cnica
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/9856 |
Resumo: | Interest in PPy-based nanocomposites has been growing due to their biocompatibility and economic viability, which make them promising for biomedical applications. Biodegradable polymers have shown potential for use as temporary matrices in studies of tissue damage therapies. Therefore, this work aimed to study the incorporation of OGR and Ag nanostructures in PPy, via direct addition of OGR in polymerization of pyrrole and direct coating of Ag by using sputtering method after PPy synthesis process, as well as to characterize the nanostructures formed by FTIR, FESEM, TEM, XRD, electrical conductivity, and to evaluate in vitro the cytotoxicity. Also, polymeric PCL/PLGA films were prepared by solvent casting, using molds without and with standardized microtopography, were characterized by FESEM, AFM, contact angle, Young's Module, and on the viability, proliferation, and adhesion of different mammalian cell types. Nanocomposite films by the addition of PPy/OGR or PPy/Ag were also prepared by solvent casting and characterized. The best method for PPy/OGR was that with sonication treatment, which doubled the electrical conductivity of the product with 20% (w/w) OGR. TEM image results confirmed the deposition of Ag nanoparticles (<32 nm) on the PPy surface and the electrical conductivity was increased 102 times relative to pure PPy. In vitro cytotoxicity testing of nanocomposites (nanocharges) suggested cell viability until 200 ?g/ mL. For polymeric films, the addition of 30% (w/w) of PLGA increased the biocompatibility and surface roughness of the PCL matrix. Films from the PCL/PLGA (70/30) blend when microstructured efficiently promoted the orientation of fibroblasts, as well as the oriented growth and proliferation of neural cells (DRG). The standardized channels in the nanocomposite films were maintained after the addition of the nanocharges, being an alternative interesting to enhance the matrix for cell orientation action. |
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Basso, Nara Regina de Souzahttp://lattes.cnpq.br/9015401653501831http://lattes.cnpq.br/3662385668383883Valente, Cristhiane Alvim2021-09-16T14:53:18Z2019-08-30http://tede2.pucrs.br/tede2/handle/tede/9856Interest in PPy-based nanocomposites has been growing due to their biocompatibility and economic viability, which make them promising for biomedical applications. Biodegradable polymers have shown potential for use as temporary matrices in studies of tissue damage therapies. Therefore, this work aimed to study the incorporation of OGR and Ag nanostructures in PPy, via direct addition of OGR in polymerization of pyrrole and direct coating of Ag by using sputtering method after PPy synthesis process, as well as to characterize the nanostructures formed by FTIR, FESEM, TEM, XRD, electrical conductivity, and to evaluate in vitro the cytotoxicity. Also, polymeric PCL/PLGA films were prepared by solvent casting, using molds without and with standardized microtopography, were characterized by FESEM, AFM, contact angle, Young's Module, and on the viability, proliferation, and adhesion of different mammalian cell types. Nanocomposite films by the addition of PPy/OGR or PPy/Ag were also prepared by solvent casting and characterized. The best method for PPy/OGR was that with sonication treatment, which doubled the electrical conductivity of the product with 20% (w/w) OGR. TEM image results confirmed the deposition of Ag nanoparticles (<32 nm) on the PPy surface and the electrical conductivity was increased 102 times relative to pure PPy. In vitro cytotoxicity testing of nanocomposites (nanocharges) suggested cell viability until 200 ?g/ mL. For polymeric films, the addition of 30% (w/w) of PLGA increased the biocompatibility and surface roughness of the PCL matrix. Films from the PCL/PLGA (70/30) blend when microstructured efficiently promoted the orientation of fibroblasts, as well as the oriented growth and proliferation of neural cells (DRG). The standardized channels in the nanocomposite films were maintained after the addition of the nanocharges, being an alternative interesting to enhance the matrix for cell orientation action.O interesse por nanocomp?sitos baseados em PPy vem crescendo devido a sua biocompatibilidade e viabilidade econ?mica, o que os tornam promissores para aplica??es biom?dicas. Pol?meros biodegrad?veis t?m demonstrado potencial de uso como matrizes tempor?rias em estudos de terapias de danos teciduais. Neste contexto, este trabalho desejou estudar a incorpora??o de nanoestruturas de OGR e Ag em PPy, via adi??o direta do OGR na polimeriza??o do pirrol e por pulveriza??o cat?dica de Ag p?s processo de s?ntese do PPy, assim como, realizar as caracteriza??es dos nanocomp?sitos formados por FTIR, MEV-FEG, TEM, DRX, condutividade el?trica, e avaliar a citotoxicidade in vitro. Al?m disso, preparou-se filmes polim?ricos de PCL/PLGA por evapora??o de solvente utilizando moldes sem e com microtopografia padronizada, os quais foram caracterizados por MEV-FEG, AFM, ?ngulo de contato, M?dulo de Young e por ensaios in vitro de viabilidade, prolifera??o e ades?o de diferentes tipos de c?lulas de mam?feros. Filmes nanocomp?sitos pela adi??o de PPy/OGR ou PPy/Ag tamb?m foram preparados por evapora??o de solvente e caracterizados. A melhor medodologia para o PPy/OGR foi aquela com tratamento de sonica??o, a qual dobrou a condutividade el?trica do produto com 20% (m/m) de OGR. Resultados de TEM confirmaram a deposi??o das nanopart?culas de Ag (< 32 nm) na superf?cie do PPy e a condutividade el?trica foi aumentada 102 vezes em rela??o ao PPy puro. Ensaios in vitro de citotoxicidade dos nanocomp?sitos (nanocargas) sugeriram viabilidade celular at? 200 ?g/mL. Para os filmes polim?ricos a adi??o de 30% (m/m) de PLGA aumentou a biocompatibilidade e a rugosidade superficial da matriz de PCL. Filmes da blenda PCL/PLGA (70/30) quando microestruturadas promoveram eficientemente a orienta??o de fibroblastos, bem como o crescimento e prolifera??o celular orientado de c?lulas neurais (DRG). Os canais nos filmes nanocomp?sitos foram mantidos ap?s adi??o das nanocargas, sendo uma alternativa de potencializa??o da matriz para a??o de orienta??o celular.Submitted by PPG Engenharia e Tecnologia de Materiais (engenharia.pg.materiais@pucrs.br) on 2021-09-14T13:46:54Z No. of bitstreams: 1 TESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf: 10410621 bytes, checksum: 9656b4f81bb48ec212348a29c0daac74 (MD5)Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2021-09-16T14:46:45Z (GMT) No. of bitstreams: 1 TESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf: 10410621 bytes, checksum: 9656b4f81bb48ec212348a29c0daac74 (MD5)Made available in DSpace on 2021-09-16T14:53:18Z (GMT). No. of bitstreams: 1 TESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf: 10410621 bytes, checksum: 9656b4f81bb48ec212348a29c0daac74 (MD5) Previous issue date: 2019-08-30Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/182043/TESE%20CRISTHIANE%20VALENTE%20VERS%c3%83O%20CORRIGIDA%20TOTAL%20FINAL.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Engenharia e Tecnologia de MateriaisPUCRSBrasilEscola Polit?cnicaPolipirrolNanocargasBlendaMicroestrutura??oNanocomp?sitoPolypyrroleNanochargersBlendMicro-StructuringNanocompositeENGENHARIASDesenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisTrabalho n?o apresenta restri??o para publica??o495391460509391966550050060045189710564848268253590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAILTESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf.jpgTESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf.jpgimage/jpeg5661http://tede2.pucrs.br/tede2/bitstream/tede/9856/4/TESE+CRISTHIANE+VALENTE+VERS%C3%83O+CORRIGIDA+TOTAL+FINAL.pdf.jpgfa5248067c54df1dcdeeb691b733b37aMD54TEXTTESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf.txtTESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdf.txttext/plain300069http://tede2.pucrs.br/tede2/bitstream/tede/9856/3/TESE+CRISTHIANE+VALENTE+VERS%C3%83O+CORRIGIDA+TOTAL+FINAL.pdf.txt5b837d6cc8b20211e7661c3b3831a152MD53ORIGINALTESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdfTESE CRISTHIANE VALENTE VERS?O CORRIGIDA TOTAL FINAL.pdfapplication/pdf10410621http://tede2.pucrs.br/tede2/bitstream/tede/9856/2/TESE+CRISTHIANE+VALENTE+VERS%C3%83O+CORRIGIDA+TOTAL+FINAL.pdf9656b4f81bb48ec212348a29c0daac74MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8590http://tede2.pucrs.br/tede2/bitstream/tede/9856/1/license.txt220e11f2d3ba5354f917c7035aadef24MD51tede/98562021-09-16 12:00:28.451oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2021-09-16T15:00:28Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
title |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
spellingShingle |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos Valente, Cristhiane Alvim Polipirrol Nanocargas Blenda Microestrutura??o Nanocomp?sito Polypyrrole Nanochargers Blend Micro-Structuring Nanocomposite ENGENHARIAS |
title_short |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
title_full |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
title_fullStr |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
title_full_unstemmed |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
title_sort |
Desenvolvimento de nanocomp?sitos polim?ricos h?bridos e biocompat?veis para aplica??o na engenharia de tecidos |
author |
Valente, Cristhiane Alvim |
author_facet |
Valente, Cristhiane Alvim |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Basso, Nara Regina de Souza |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9015401653501831 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3662385668383883 |
dc.contributor.author.fl_str_mv |
Valente, Cristhiane Alvim |
contributor_str_mv |
Basso, Nara Regina de Souza |
dc.subject.por.fl_str_mv |
Polipirrol Nanocargas Blenda Microestrutura??o Nanocomp?sito |
topic |
Polipirrol Nanocargas Blenda Microestrutura??o Nanocomp?sito Polypyrrole Nanochargers Blend Micro-Structuring Nanocomposite ENGENHARIAS |
dc.subject.eng.fl_str_mv |
Polypyrrole Nanochargers Blend Micro-Structuring Nanocomposite |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS |
description |
Interest in PPy-based nanocomposites has been growing due to their biocompatibility and economic viability, which make them promising for biomedical applications. Biodegradable polymers have shown potential for use as temporary matrices in studies of tissue damage therapies. Therefore, this work aimed to study the incorporation of OGR and Ag nanostructures in PPy, via direct addition of OGR in polymerization of pyrrole and direct coating of Ag by using sputtering method after PPy synthesis process, as well as to characterize the nanostructures formed by FTIR, FESEM, TEM, XRD, electrical conductivity, and to evaluate in vitro the cytotoxicity. Also, polymeric PCL/PLGA films were prepared by solvent casting, using molds without and with standardized microtopography, were characterized by FESEM, AFM, contact angle, Young's Module, and on the viability, proliferation, and adhesion of different mammalian cell types. Nanocomposite films by the addition of PPy/OGR or PPy/Ag were also prepared by solvent casting and characterized. The best method for PPy/OGR was that with sonication treatment, which doubled the electrical conductivity of the product with 20% (w/w) OGR. TEM image results confirmed the deposition of Ag nanoparticles (<32 nm) on the PPy surface and the electrical conductivity was increased 102 times relative to pure PPy. In vitro cytotoxicity testing of nanocomposites (nanocharges) suggested cell viability until 200 ?g/ mL. For polymeric films, the addition of 30% (w/w) of PLGA increased the biocompatibility and surface roughness of the PCL matrix. Films from the PCL/PLGA (70/30) blend when microstructured efficiently promoted the orientation of fibroblasts, as well as the oriented growth and proliferation of neural cells (DRG). The standardized channels in the nanocomposite films were maintained after the addition of the nanocharges, being an alternative interesting to enhance the matrix for cell orientation action. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-08-30 |
dc.date.accessioned.fl_str_mv |
2021-09-16T14:53:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://tede2.pucrs.br/tede2/handle/tede/9856 |
url |
http://tede2.pucrs.br/tede2/handle/tede/9856 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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4953914605093919665 |
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500 500 600 |
dc.relation.cnpq.fl_str_mv |
4518971056484826825 |
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3590462550136975366 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais |
dc.publisher.initials.fl_str_mv |
PUCRS |
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Brasil |
dc.publisher.department.fl_str_mv |
Escola Polit?cnica |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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