Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Odontologia
|
Departamento: |
Faculdade de Odontologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/6502 |
Resumo: | The aim of this study was to evaluate dental pulp and furcal periodontal tissue responses to mineral trioxide aggregate (MTA) and to a calcium silicate-based material (Biodentine?). The lower first molars in hundred forty (140) male Wistar rats were divided into groups according to the experimental site and period (14 and 21 days) testing two materials (n=8 per group/period in pulpotomy and n=6 per group/period in furcal perforation). Groups capped and sealed with MTA or Biodentine? were restored with silver amalgam; in an additional test group, teeth were capped and restored with Biodentine?. Teeth capped with gutta-percha and restored with silver amalgam served as a positive control and untouched teeth were negative control. Histological evaluation of pulp responses and macroscopic examination of coronal sealing were performed. Inflammation extension and intensity, degenerative events and formation of mineralized tissue barrier were assessed. In the experiment of furcal perforations, inflammation extension and intensity, bone resorption and cement formation were assessed histologically. All results underwent ANOVA and Tukey post hoc tests (p<.05). In pulpotomy, Biodentine? and MTA presented satisfactory results, which were characterized by a mild inflammatory response (p<.0001) and more pronounced formation of mineralized barrier (p<.0001) compared to teeth capped with gutta-percha. When used as a restorative material, Biodentine? kept coronal sealing in only 37.5% of the samples. As to furcal perforations, Biodentine? and MTA presented satisfactory results, which were characterized by a milder inflammatory response compared to the control, regardless of the material used for coronal sealing and of the experimental period evaluated (p<.0001). All test groups showed less bone resorption than the positive control after 21 days (p<.05), being this difference more pronounced in teeth restored with silver amalgam. Cement repair occurred in 30% of MTA and Biodentine? samples, and was not detected in any specimen of the positive control group. In conclusion, Biodentine? and MTA promoted similar responses when used to pulp capping and furcal perforation sealing. On the other hand, the use of Biodentine? as temporary restorative material did not promote efficient coronal sealing. Although further investigations are required, Biodentine? can be considered an alternative for pulpotomy and furcal perfuration. |
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Figueiredo, Jos? Antonio Poli de402.322.200-34http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721106A2Scarparo, Roberta Kochenborgerhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4736351Y4578.649.346-68http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705645P9Reis, Magda de Sousa2016-02-12T17:20:18Z2015-10-15http://tede2.pucrs.br/tede2/handle/tede/6502The aim of this study was to evaluate dental pulp and furcal periodontal tissue responses to mineral trioxide aggregate (MTA) and to a calcium silicate-based material (Biodentine?). The lower first molars in hundred forty (140) male Wistar rats were divided into groups according to the experimental site and period (14 and 21 days) testing two materials (n=8 per group/period in pulpotomy and n=6 per group/period in furcal perforation). Groups capped and sealed with MTA or Biodentine? were restored with silver amalgam; in an additional test group, teeth were capped and restored with Biodentine?. Teeth capped with gutta-percha and restored with silver amalgam served as a positive control and untouched teeth were negative control. Histological evaluation of pulp responses and macroscopic examination of coronal sealing were performed. Inflammation extension and intensity, degenerative events and formation of mineralized tissue barrier were assessed. In the experiment of furcal perforations, inflammation extension and intensity, bone resorption and cement formation were assessed histologically. All results underwent ANOVA and Tukey post hoc tests (p<.05). In pulpotomy, Biodentine? and MTA presented satisfactory results, which were characterized by a mild inflammatory response (p<.0001) and more pronounced formation of mineralized barrier (p<.0001) compared to teeth capped with gutta-percha. When used as a restorative material, Biodentine? kept coronal sealing in only 37.5% of the samples. As to furcal perforations, Biodentine? and MTA presented satisfactory results, which were characterized by a milder inflammatory response compared to the control, regardless of the material used for coronal sealing and of the experimental period evaluated (p<.0001). All test groups showed less bone resorption than the positive control after 21 days (p<.05), being this difference more pronounced in teeth restored with silver amalgam. Cement repair occurred in 30% of MTA and Biodentine? samples, and was not detected in any specimen of the positive control group. In conclusion, Biodentine? and MTA promoted similar responses when used to pulp capping and furcal perforation sealing. On the other hand, the use of Biodentine? as temporary restorative material did not promote efficient coronal sealing. Although further investigations are required, Biodentine? can be considered an alternative for pulpotomy and furcal perfuration.O objetivo deste estudo foi comparar a resposta tecidual, ap?s pulpotomias e perfura??es de furca, utilizando agregado tri?xido de mineral (MTA) e o material ? base de silicato tric?lcico (Biodentine?). Os primeiros molares inferiores de 140 ratos Wistar machos foram utilizados em dois tempos (14 e 21 dias) e divididos em grupos conforme o experimento e materiais utilizados (pulpotomia n=8 e perfura??o de furca n=6). Nos dois experimentos, grupos capeados e selados com MTA e Biodentine? foram restaurados com am?lgama de prata; um grupo adicional utilizou somente Biodentine? e o controle positivo recebeu guta-percha e restaura??o de am?lgama; dentes intactos serviram de controle negativo. No experimento de pulpotomias, realizou-se exame macrosc?pico e radiogr?fico do selamento coron?rio e exame histol?gico dos eventos inflamat?rios (intensidade e extens?o), degenerativos e de forma??o de barreira mineralizada. No experimento de perfura??es de furca, foram avaliados histologicamente os eventos inflamat?rios (intensidade e extens?o), de reabsor??o ?ssea e reparo de cemento. Os dados foram comparados atrav?s da an?lise de vari?ncia (ANOVA) e posthoc de Tukey (p<.05). Nas pulpotomias, o Biodentine? e MTA apresentaram resultados satisfat?rios, com menor resposta inflamat?ria (p<.0001) e pronunciada forma??o de barreira mineralizada (p<.0001) comparada aos dentes do controle positivo. O Biodentine?, utilizado como restaura??o, manteve o selamento coron?rio em apenas 37.5% das amostras. Nas perfura??es de furca, o Biodentine e MTA apresentaram resultados satisfat?rios, caracterizados por uma resposta inflamat?ria mais suave em compara??o ao controle positivo, independentemente do material utilizado para o selamento coron?rio e per?odo experimental avaliado (p<.0001). Todos os grupos testes mostraram menor reabsor??o ?ssea do que o controle positivo ap?s 21 dias (p<.05), sendo esta diferen?a mais acentuada em dentes restaurados com am?lgama de prata. A repara??o de cemento ocorreu em 30% das amostras de MTA e Biodentine, e n?o foi detectada em qualquer amostra do grupo do controle positivo. Concluiu-se que houve respostas similares ap?s o uso de Biodentine? e MTA no capeamento da polpa exposta e selamento de perfura??es de furca. Por outro lado, o uso do Biodentine? como material restaurador provis?rio n?o promoveu selamento coron?rio eficiente. Embora sejam necess?rias novas investiga??es, o Biodentine deve ser considerado como uma alternativa frente aos tratamentos propostos.Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-02-12T17:20:18Z No. of bitstreams: 1 TES_MAGDA_DE_SOUSA_REIS_COMPLETO.pdf: 2952696 bytes, checksum: 9a8e4368b83491c5888a26990b0c9ddc (MD5)Made available in DSpace on 2016-02-12T17:20:18Z (GMT). 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dc.title.por.fl_str_mv |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
dc.title.alternative.eng.fl_str_mv |
Biological effect of Biodentine? and MTA on rats? dental pulp and furcal periodontal tissues |
title |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
spellingShingle |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos Reis, Magda de Sousa ODONTOLOGIA ENDODONTIA MATERIAIS DENT?RIOS COMPOSTOS BIOATIVOS REPARO TECIDUAL (ODONTOLOGIA) CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
title_full |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
title_fullStr |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
title_full_unstemmed |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
title_sort |
Efeito biol?gico do Biodentine? e do MTA sobre exposi??o de tecido pulpar e periodontal da furca : estudo em ratos |
author |
Reis, Magda de Sousa |
author_facet |
Reis, Magda de Sousa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Figueiredo, Jos? Antonio Poli de |
dc.contributor.advisor1ID.fl_str_mv |
402.322.200-34 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721106A2 |
dc.contributor.advisor-co1.fl_str_mv |
Scarparo, Roberta Kochenborger |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4736351Y4 |
dc.contributor.authorID.fl_str_mv |
578.649.346-68 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705645P9 |
dc.contributor.author.fl_str_mv |
Reis, Magda de Sousa |
contributor_str_mv |
Figueiredo, Jos? Antonio Poli de Scarparo, Roberta Kochenborger |
dc.subject.por.fl_str_mv |
ODONTOLOGIA ENDODONTIA MATERIAIS DENT?RIOS COMPOSTOS BIOATIVOS REPARO TECIDUAL (ODONTOLOGIA) |
topic |
ODONTOLOGIA ENDODONTIA MATERIAIS DENT?RIOS COMPOSTOS BIOATIVOS REPARO TECIDUAL (ODONTOLOGIA) CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The aim of this study was to evaluate dental pulp and furcal periodontal tissue responses to mineral trioxide aggregate (MTA) and to a calcium silicate-based material (Biodentine?). The lower first molars in hundred forty (140) male Wistar rats were divided into groups according to the experimental site and period (14 and 21 days) testing two materials (n=8 per group/period in pulpotomy and n=6 per group/period in furcal perforation). Groups capped and sealed with MTA or Biodentine? were restored with silver amalgam; in an additional test group, teeth were capped and restored with Biodentine?. Teeth capped with gutta-percha and restored with silver amalgam served as a positive control and untouched teeth were negative control. Histological evaluation of pulp responses and macroscopic examination of coronal sealing were performed. Inflammation extension and intensity, degenerative events and formation of mineralized tissue barrier were assessed. In the experiment of furcal perforations, inflammation extension and intensity, bone resorption and cement formation were assessed histologically. All results underwent ANOVA and Tukey post hoc tests (p<.05). In pulpotomy, Biodentine? and MTA presented satisfactory results, which were characterized by a mild inflammatory response (p<.0001) and more pronounced formation of mineralized barrier (p<.0001) compared to teeth capped with gutta-percha. When used as a restorative material, Biodentine? kept coronal sealing in only 37.5% of the samples. As to furcal perforations, Biodentine? and MTA presented satisfactory results, which were characterized by a milder inflammatory response compared to the control, regardless of the material used for coronal sealing and of the experimental period evaluated (p<.0001). All test groups showed less bone resorption than the positive control after 21 days (p<.05), being this difference more pronounced in teeth restored with silver amalgam. Cement repair occurred in 30% of MTA and Biodentine? samples, and was not detected in any specimen of the positive control group. In conclusion, Biodentine? and MTA promoted similar responses when used to pulp capping and furcal perforation sealing. On the other hand, the use of Biodentine? as temporary restorative material did not promote efficient coronal sealing. Although further investigations are required, Biodentine? can be considered an alternative for pulpotomy and furcal perfuration. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-10-15 |
dc.date.accessioned.fl_str_mv |
2016-02-12T17:20:18Z |
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info:eu-repo/semantics/publishedVersion |
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http://tede2.pucrs.br/tede2/handle/tede/6502 |
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http://tede2.pucrs.br/tede2/handle/tede/6502 |
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por |
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por |
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600 600 600 |
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openAccess |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Odontologia |
dc.publisher.initials.fl_str_mv |
PUCRS |
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Brasil |
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Faculdade de Odontologia |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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