Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas

Detalhes bibliográficos
Ano de defesa: 2005
Autor(a) principal: Mattos, Valéria Gonçalves Albieri
Orientador(a): Nonaka, Keico Okino lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Carlos
Programa de Pós-Graduação: Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF
Departamento: Não Informado pela instituição
País: BR
Palavras-chave em Português:
Medicina experimental - rata
Fêmur
Sistema nervoso autônomo
Ovariectomia
Osteoporose
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::FISIOLOGIA
Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/1384
Resumo: The bone sympathetic innervation influences the osteoblastic activity and supports the participation of Autonomic Nervous System on the bone formation regulation. Treatment with guanetidine promotes denervation of the sympathetic system in humans as well as animals. This work aims at investigating participation of the sympathetic system on the femur biology of ovariectomized adult rats. For this purpose, physical and biomechanical parameters, in addition to plasma and femur calcium were analyzed. Forty one, 4-month aged female Wistar rats were divided into the following experimental groups: basal (n=9), intact (n=8) treated with 1ml saline/Kg BW, castrated and treated with 1ml saline/Kg BW (n=7), castrated and treated with 37 mg guanetidine/Kg BW during six weeks (n=9) and castrated and treated with 40 mg guanetidine/Kg BW during eight weeks (n=8). The treatment was performed during six days/week. The animals were housed under controlled temperature (22 ± 1°C) and light (lights on between 8 a.m. and 6 p.m. daily) conditions. The rats had free access to food and tap water. After 8 weeks the animals were sacrificed. It was determined that ovariectomy induced higher BW gain and osteopenia. The denervation promoted by guanetidine did not protect the bone tissue from the deleterious effects on account of castration as demonstrated by the values of physical, biochemical and biomechanical parameters.
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spelling Mattos, Valéria Gonçalves AlbieriNonaka, Keico Okinohttp://lattes.cnpq.br/455103677573829671ba6fe8-f9f1-4446-a1f6-29c593d9e2262016-06-02T19:23:03Z2007-10-192016-06-02T19:23:03Z2005-09-16MATTOS, Valéria Gonçalves Albieri. Effects of sympathectomy promoted by guanetidine on femurs of ovariectomized rats. 2005. 81 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2005.https://repositorio.ufscar.br/handle/20.500.14289/1384The bone sympathetic innervation influences the osteoblastic activity and supports the participation of Autonomic Nervous System on the bone formation regulation. Treatment with guanetidine promotes denervation of the sympathetic system in humans as well as animals. This work aims at investigating participation of the sympathetic system on the femur biology of ovariectomized adult rats. For this purpose, physical and biomechanical parameters, in addition to plasma and femur calcium were analyzed. Forty one, 4-month aged female Wistar rats were divided into the following experimental groups: basal (n=9), intact (n=8) treated with 1ml saline/Kg BW, castrated and treated with 1ml saline/Kg BW (n=7), castrated and treated with 37 mg guanetidine/Kg BW during six weeks (n=9) and castrated and treated with 40 mg guanetidine/Kg BW during eight weeks (n=8). The treatment was performed during six days/week. The animals were housed under controlled temperature (22 ± 1°C) and light (lights on between 8 a.m. and 6 p.m. daily) conditions. The rats had free access to food and tap water. After 8 weeks the animals were sacrificed. It was determined that ovariectomy induced higher BW gain and osteopenia. The denervation promoted by guanetidine did not protect the bone tissue from the deleterious effects on account of castration as demonstrated by the values of physical, biochemical and biomechanical parameters.A inervação simpática do osso influencia a atividade osteoblástica e providencia suporte para o papel do Sistema Nervoso Autônomo sobre a regulação da formação óssea. O tratamento com Guanetidina promove a desnervação do Sistema Nervoso Simpático, tanto em humanos quanto em animais. O objetivo do presente estudo foi investigar a atuação do Sistema Nervoso Simpático sobre fêmures de ratas adultas e castradas, por meio da análise de parâmetros biométricos, físicos, biomecânicos e concentração de cálcio no plasma e femur dos animais experimentais. Quarenta e uma ratas Wistar com 4 meses de idade foram divididas nos seguintes grupos experimentais: Basal (n=9), Intacto (n=8) salina 0,1ml/Kg/PC, Castrado (n=7) - salina 0,1ml/Kg/PC, Castrado e Tratado por 6 semanas (n=9) Guanetidina 37mg/Kg PC, Castrado e Tratado por 8 semanas (n=8) Guanetidina 40mg/Kg PC. O tratamento foi realizado durante seis dias por semana, os animais foram mantidos sob um regime de luz (10 horas claro/ 14 horas escuro), com ração comercial e água ad libitum . Após 8 semanas, os animais foram sacrificados. A castração aumentou o ganho de Peso Corporal e induziu osteopenia. A não-atuação do sistema nervoso simpático periférico sobre o tecido ósseo, ocasionada pela simpatectomia induzida pela guanetidina, não promoveu proteção aos efeitos deletérios da castração, fato indicado pelos parâmetros de densidade óssea, densidade mineral, peso das cinzas, porcentagem de água, porcentagem de material mineral, rigidez e cálcio plasmático.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarBRMedicina experimental - rataFêmurSistema nervoso autônomoOvariectomiaOsteoporoseCIENCIAS BIOLOGICAS::FISIOLOGIAEfeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradasEffects of sympathectomy promoted by guanetidine on femurs of ovariectomized ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisebecdfb1-cdc6-42ca-b661-e018c16ea74cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARTEXTDissVGAM.pdf.txtDissVGAM.pdf.txtExtracted texttext/plain102158https://repositorio.ufscar.br/bitstreams/03c265f4-fa9f-49a7-9d9f-a85ef5f0c8f2/download354a79ae12d480e3065cb9a20bf285d2MD53falseAnonymousREADORIGINALDissVGAM.pdfapplication/pdf1826272https://repositorio.ufscar.br/bitstreams/50ffeed5-5783-4f61-bbc7-f55189398a27/downloaddcc405aee38cab9e95539b226c322ce9MD51trueAnonymousREADTHUMBNAILDissVGAM.pdf.jpgDissVGAM.pdf.jpgIM Thumbnailimage/jpeg6891https://repositorio.ufscar.br/bitstreams/2b4bb1b1-43b9-489b-b5e1-73b63b05bdda/downloadef07b6b36c11ec2eeeac7f0d2f9ca15bMD52falseAnonymousREAD20.500.14289/13842025-02-06 04:04:47.635open.accessoai:repositorio.ufscar.br:20.500.14289/1384https://repositorio.ufscar.brRepositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestrepositorio.sibi@ufscar.bropendoar:43222025-02-06T07:04:47Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
dc.title.alternative.eng.fl_str_mv Effects of sympathectomy promoted by guanetidine on femurs of ovariectomized rats
title Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
spellingShingle Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
Mattos, Valéria Gonçalves Albieri
Medicina experimental - rata
Fêmur
Sistema nervoso autônomo
Ovariectomia
Osteoporose
CIENCIAS BIOLOGICAS::FISIOLOGIA
title_short Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
title_full Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
title_fullStr Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
title_full_unstemmed Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
title_sort Efeito da simpatectomia provocada pela guanetidina sobre fêmures de ratas castradas
author Mattos, Valéria Gonçalves Albieri
author_facet Mattos, Valéria Gonçalves Albieri
author_role author
dc.contributor.author.fl_str_mv Mattos, Valéria Gonçalves Albieri
dc.contributor.advisor1.fl_str_mv Nonaka, Keico Okino
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4551036775738296
dc.contributor.authorID.fl_str_mv 71ba6fe8-f9f1-4446-a1f6-29c593d9e226
contributor_str_mv Nonaka, Keico Okino
dc.subject.por.fl_str_mv Medicina experimental - rata
Fêmur
Sistema nervoso autônomo
Ovariectomia
Osteoporose
topic Medicina experimental - rata
Fêmur
Sistema nervoso autônomo
Ovariectomia
Osteoporose
CIENCIAS BIOLOGICAS::FISIOLOGIA
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::FISIOLOGIA
description The bone sympathetic innervation influences the osteoblastic activity and supports the participation of Autonomic Nervous System on the bone formation regulation. Treatment with guanetidine promotes denervation of the sympathetic system in humans as well as animals. This work aims at investigating participation of the sympathetic system on the femur biology of ovariectomized adult rats. For this purpose, physical and biomechanical parameters, in addition to plasma and femur calcium were analyzed. Forty one, 4-month aged female Wistar rats were divided into the following experimental groups: basal (n=9), intact (n=8) treated with 1ml saline/Kg BW, castrated and treated with 1ml saline/Kg BW (n=7), castrated and treated with 37 mg guanetidine/Kg BW during six weeks (n=9) and castrated and treated with 40 mg guanetidine/Kg BW during eight weeks (n=8). The treatment was performed during six days/week. The animals were housed under controlled temperature (22 ± 1°C) and light (lights on between 8 a.m. and 6 p.m. daily) conditions. The rats had free access to food and tap water. After 8 weeks the animals were sacrificed. It was determined that ovariectomy induced higher BW gain and osteopenia. The denervation promoted by guanetidine did not protect the bone tissue from the deleterious effects on account of castration as demonstrated by the values of physical, biochemical and biomechanical parameters.
publishDate 2005
dc.date.issued.fl_str_mv 2005-09-16
dc.date.available.fl_str_mv 2007-10-19
2016-06-02T19:23:03Z
dc.date.accessioned.fl_str_mv 2016-06-02T19:23:03Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MATTOS, Valéria Gonçalves Albieri. Effects of sympathectomy promoted by guanetidine on femurs of ovariectomized rats. 2005. 81 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2005.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/20.500.14289/1384
identifier_str_mv MATTOS, Valéria Gonçalves Albieri. Effects of sympathectomy promoted by guanetidine on femurs of ovariectomized rats. 2005. 81 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2005.
url https://repositorio.ufscar.br/handle/20.500.14289/1384
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