Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Costa, Cleide Caldas
Orientador(a): Duarte, Iolanda Cristina Silveira lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Carlos
Câmpus Sorocaba
Programa de Pós-Graduação: Programa de Pós-Graduação em Biotecnologia e Monitoramento Ambiental - PPGBMA-So
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
BIT
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/9965
Resumo: Metalworking Fluids (MWF) is the class of oils and liquids used to lubricate, reduce heat and friction between the tool and parts, helping the parts machining at the time of cutting. Contamination from the dilution water and the nutrients present in the MWF can contribute to the growth of microorganisms in the fluid. These microorganisms can cause diseases in operators and also corrosion of machine components, tools and parts shortening the life of the MWF, due to the loss of its physicochemical properties. The objective of this study was to monitor the microbiological conditions of metal cutting in seven central circulation systems of a metallurgical plant. Monitoring was realized weekly for seven months. The MWF samples were analyzed for concentrations of biocidal actives N-Butyl-1,2-benzisothiazoline-3-one (NBBIT) and 1,2-benzisothiazoline-3-one (BIT) by liquid chromatography and counting of microorganisms by pour plating in specific mediums for total mesophilic aerobic bacteria, yeasts and filamentous fungi. The presence of sulfate reducing bacteria (BRS) was perform at seven weeks intervals. Bacteria and yeasts contaminated all central circulations systems and only one of the central had no BRS presence. The MWF evaluated showed no contamination by filament fungi. The concentrations of biocidal active NBBIT and BIT were below those mentioned in the literature as efficient in the control of contamination. Thus, it was concluded that the analyzed metallurgy is not following the dosage of established biocides active 200 ppm to NBBIT and 2.500 ppm to BIT, this is leading to contaminations that can be harmful to the operators. The suggestion is that the company take preventive action measures, such as weekly monitoring and corrective actions like plant audits to detect and control the microbiological load of the central circulations system.
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spelling Costa, Cleide CaldasDuarte, Iolanda Cristina Silveirahttp://lattes.cnpq.br/2626035341263254dos Santos, André Cordeiro Alveshttp://lattes.cnpq.br/2131880667704281http://lattes.cnpq.br/5310584416241411e49a4502-6d63-441c-9649-79ec458302322018-05-10T19:27:38Z2018-05-10T19:27:38Z2018-03-09COSTA, Cleide Caldas. Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.. 2018. Dissertação (Mestrado em Biotecnologia e Monitoramento Ambiental) – Universidade Federal de São Carlos, Sorocaba, 2018. Disponível em: https://repositorio.ufscar.br/handle/20.500.14289/9965.https://repositorio.ufscar.br/handle/20.500.14289/9965Metalworking Fluids (MWF) is the class of oils and liquids used to lubricate, reduce heat and friction between the tool and parts, helping the parts machining at the time of cutting. Contamination from the dilution water and the nutrients present in the MWF can contribute to the growth of microorganisms in the fluid. These microorganisms can cause diseases in operators and also corrosion of machine components, tools and parts shortening the life of the MWF, due to the loss of its physicochemical properties. The objective of this study was to monitor the microbiological conditions of metal cutting in seven central circulation systems of a metallurgical plant. Monitoring was realized weekly for seven months. The MWF samples were analyzed for concentrations of biocidal actives N-Butyl-1,2-benzisothiazoline-3-one (NBBIT) and 1,2-benzisothiazoline-3-one (BIT) by liquid chromatography and counting of microorganisms by pour plating in specific mediums for total mesophilic aerobic bacteria, yeasts and filamentous fungi. The presence of sulfate reducing bacteria (BRS) was perform at seven weeks intervals. Bacteria and yeasts contaminated all central circulations systems and only one of the central had no BRS presence. The MWF evaluated showed no contamination by filament fungi. The concentrations of biocidal active NBBIT and BIT were below those mentioned in the literature as efficient in the control of contamination. Thus, it was concluded that the analyzed metallurgy is not following the dosage of established biocides active 200 ppm to NBBIT and 2.500 ppm to BIT, this is leading to contaminations that can be harmful to the operators. The suggestion is that the company take preventive action measures, such as weekly monitoring and corrective actions like plant audits to detect and control the microbiological load of the central circulations system.Fluidos para cortes de metais (Metalworking Fluids - MWF) é a classe de óleos e líquidos utilizados para lubrificar, reduzir o calor e o atrito entre a ferramenta e a peça, auxiliando a usinagem das peças no momento do corte. A contaminação proveniente da água de diluição e os nutrientes presentes no MWF podem contribuir para o crescimento de micro-organismos no fluido. Estes micro-organismos podem causar doenças em operadores, corrosão de componentes de máquinas, ferramentas, peças e diminuição da vida útil do MWF, devido à perda de suas propriedades físico-químicas. O objetivo deste trabalho, foi monitorar as condições microbiológicas de fluido para cortes de metais em sete centrais de uma metalúrgica. O monitoramento foi realizado semanalmente, durante sete meses. As amostras do MWF foram analisadas quanto às concentrações de ativos biocidas N-butil-1,2-benzisotiazolin-3-ona (NBBIT) e 1,2-benzisotiazolin-3-ona (BIT), por cromatografia líquida e à contagem de micro-organismos por plaqueamento em meios específicos para bactérias mesófilas aeróbias, fungos filamentosos e leveduras. A presença de bactérias redutoras de sulfato (BRS), foi verificada em intervalo de sete semanas. As concentrações de ativos biocidas NBBIT e BIT estiveram abaixo dos mencionados pela literatura como eficientes no controle de contaminação. Todas as centrais de sistema de circulação, apresentaram contaminação por bactérias, leveduras e apenas uma das centrais não apresentou presença de BRS. Os MWF avaliados não apresentaram contaminação por fungos filamentos. Dessa forma, concluiu-se, que a metalúrgica analisada não está seguindo a dosagem de ativos biocidas estabelecidas de 200 ppm para NBBIT e 2.500 ppm para BIT e isto está acarretando contaminações que podem ser prejudiciais aos operadores. Sugere-se que a empresa tome medidas de ações preventivas, adotando o monitoramento semanal, ações corretivas como auditorias de planta para detectar e controlar a carga microbiológica das centrais.Não recebi financiamentoporUniversidade Federal de São CarlosCâmpus SorocabaPrograma de Pós-Graduação em Biotecnologia e Monitoramento Ambiental - PPGBMA-SoUFSCarFluidos para cortes de metaisNBBITBITBactériasLevedurasBactérias redutoras de sulfatoFluidos em metaloplastiaFluids for cutting metalsBacteriaYeastSulfate Reducing BacteriaMetal-working lubricantsCIENCIAS BIOLOGICAS::MICROBIOLOGIAAvaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.Evaluation of microbial contamination in fluid for metal cutting in the metallurgical industry.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline6006002195fc4e-4332-4510-b250-2858d4a9df1einfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDISSERTACAO VERSÃO FINAL CLEIDE CALDAS COSTA_ (002).pdfDISSERTACAO VERSÃO FINAL CLEIDE CALDAS COSTA_ (002).pdfDissertação versão final Cleide Caldas Costaapplication/pdf1539878https://repositorio.ufscar.br/bitstreams/016f184b-df20-4dbe-a936-7f2d773a4b92/downloade865b2ac2cb7f30c5dad1f2626b0a5e6MD51trueAnonymousREADficha_catalografica Cleide.pdfficha_catalografica Cleide.pdfficha catalografica Cleide Caldas Costaapplication/pdf5951https://repositorio.ufscar.br/bitstreams/1fec0dd5-0975-4907-96ed-303a5a5cb654/download9e4832d956021eb8b67f3a3e273aa939MD52falseAnonymousREADRI Autoarquivamento aprovado Dra Iolanda.pdfRI Autoarquivamento aprovado Dra Iolanda.pdfAprovação de auto arquivamentoapplication/pdf254822https://repositorio.ufscar.br/bitstreams/d50bdc4e-8311-4f49-9058-fcafb1c44ce1/download57cb6dac006ab1be94ca37ed2f134042MD54falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
dc.title.alternative.eng.fl_str_mv Evaluation of microbial contamination in fluid for metal cutting in the metallurgical industry.
title Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
spellingShingle Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
Costa, Cleide Caldas
Fluidos para cortes de metais
NBBIT
BIT
Bactérias
Leveduras
Bactérias redutoras de sulfato
Fluidos em metaloplastia
Fluids for cutting metals
Bacteria
Yeast
Sulfate Reducing Bacteria
Metal-working lubricants
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
title_short Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
title_full Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
title_fullStr Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
title_full_unstemmed Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
title_sort Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.
author Costa, Cleide Caldas
author_facet Costa, Cleide Caldas
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/5310584416241411
dc.contributor.author.fl_str_mv Costa, Cleide Caldas
dc.contributor.advisor1.fl_str_mv Duarte, Iolanda Cristina Silveira
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2626035341263254
dc.contributor.advisor-co1.fl_str_mv dos Santos, André Cordeiro Alves
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/2131880667704281
dc.contributor.authorID.fl_str_mv e49a4502-6d63-441c-9649-79ec45830232
contributor_str_mv Duarte, Iolanda Cristina Silveira
dos Santos, André Cordeiro Alves
dc.subject.por.fl_str_mv Fluidos para cortes de metais
NBBIT
BIT
Bactérias
Leveduras
Bactérias redutoras de sulfato
Fluidos em metaloplastia
topic Fluidos para cortes de metais
NBBIT
BIT
Bactérias
Leveduras
Bactérias redutoras de sulfato
Fluidos em metaloplastia
Fluids for cutting metals
Bacteria
Yeast
Sulfate Reducing Bacteria
Metal-working lubricants
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
dc.subject.eng.fl_str_mv Fluids for cutting metals
Bacteria
Yeast
Sulfate Reducing Bacteria
Metal-working lubricants
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::MICROBIOLOGIA
description Metalworking Fluids (MWF) is the class of oils and liquids used to lubricate, reduce heat and friction between the tool and parts, helping the parts machining at the time of cutting. Contamination from the dilution water and the nutrients present in the MWF can contribute to the growth of microorganisms in the fluid. These microorganisms can cause diseases in operators and also corrosion of machine components, tools and parts shortening the life of the MWF, due to the loss of its physicochemical properties. The objective of this study was to monitor the microbiological conditions of metal cutting in seven central circulation systems of a metallurgical plant. Monitoring was realized weekly for seven months. The MWF samples were analyzed for concentrations of biocidal actives N-Butyl-1,2-benzisothiazoline-3-one (NBBIT) and 1,2-benzisothiazoline-3-one (BIT) by liquid chromatography and counting of microorganisms by pour plating in specific mediums for total mesophilic aerobic bacteria, yeasts and filamentous fungi. The presence of sulfate reducing bacteria (BRS) was perform at seven weeks intervals. Bacteria and yeasts contaminated all central circulations systems and only one of the central had no BRS presence. The MWF evaluated showed no contamination by filament fungi. The concentrations of biocidal active NBBIT and BIT were below those mentioned in the literature as efficient in the control of contamination. Thus, it was concluded that the analyzed metallurgy is not following the dosage of established biocides active 200 ppm to NBBIT and 2.500 ppm to BIT, this is leading to contaminations that can be harmful to the operators. The suggestion is that the company take preventive action measures, such as weekly monitoring and corrective actions like plant audits to detect and control the microbiological load of the central circulations system.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-05-10T19:27:38Z
dc.date.available.fl_str_mv 2018-05-10T19:27:38Z
dc.date.issued.fl_str_mv 2018-03-09
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Cleide Caldas. Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.. 2018. Dissertação (Mestrado em Biotecnologia e Monitoramento Ambiental) – Universidade Federal de São Carlos, Sorocaba, 2018. Disponível em: https://repositorio.ufscar.br/handle/20.500.14289/9965.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/20.500.14289/9965
identifier_str_mv COSTA, Cleide Caldas. Avaliação da contaminação microbiana em fluido para cortes de metais em indústria metalúrgica.. 2018. Dissertação (Mestrado em Biotecnologia e Monitoramento Ambiental) – Universidade Federal de São Carlos, Sorocaba, 2018. Disponível em: https://repositorio.ufscar.br/handle/20.500.14289/9965.
url https://repositorio.ufscar.br/handle/20.500.14289/9965
dc.language.iso.fl_str_mv por
language por
dc.relation.confidence.fl_str_mv 600
600
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus Sorocaba
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biotecnologia e Monitoramento Ambiental - PPGBMA-So
dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus Sorocaba
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institution UFSCAR
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collection Repositório Institucional da UFSCAR
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repository.name.fl_str_mv Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv repositorio.sibi@ufscar.br
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