Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre
Ano de defesa: | 2018 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ecologia e Recursos Naturais - PPGERN
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/10444 |
Resumo: | The phytoplankton community is composed of microalgae that support food chains in aquatic ecosystems. In these environments they are immersed in a complex matrix of organic and inorganic substances. In some situations, they are still subject to a range of anthropogenic wastes that end up entering aquatic environments, such as nanoparticles (NPs). Due to the unique characteristics of NPs, their use is intense, even if neither use nor disposal is regularized by competent governamental agencies. Toxicological studies on the effect of NPs on phytoplankton have controversial results. The toxicity of NPs is related to their reactivity, which depends on the size of the particles, formation of aggregates, availability of organic matter, among other factors. In this study, we first sought to understand the dynamics of NPs in algal cultures and followed investigating on the physiological effects of copper NPs (NPs-Cu) on two freshwater Chlorophyta (Chlorella sorokiniana and Ankistrodesmus densus) exposed to nominal NPs-Cu concentrations estimated for the environment (0.57 to 12.70 μg L-1) and above these (up to 635.0 μg L-1). We analyzed parameters related to growth, photosynthesis and intracellular biomolecules under controlled laboratory conditions at 72 h cultures. The results showed that the population growth rate was affected in both microalgae species. The intensity of the photosynthetic processes varied according to the microalgae, with C. sorokiniana being more resistant than A. densus that presented reductions in photosystem II quantum yield, electron transport rates and consequentely in the estimated gross primary productivity. The biochemical composition (proteins, carbohydrates and lipids) was affected in the presence of NPs-Cu in both microalgae, but at environmentally relevant concentrations of NPs-Cu only carbohydrates were affected. Protein synthesis was stimulated at the higher end of the NPs-Cu concentration range, suggesting the production of antioxidant proteins associated with cell detoxification. This study is a contribution to the knowledge of the toxicology of NPs to freshwater microalgae and their possible consequence in natural environment. |
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Barreto, Daniela MarianoLombardi, Ana Teresahttp://lattes.cnpq.br/6737850858443813Tonietto, Alessandra Emanuelehttp://lattes.cnpq.br/3056194008699912http://lattes.cnpq.br/07256481677564262018-09-10T12:17:28Z2018-09-10T12:17:28Z2018-08-20BARRETO, Daniela Mariano. Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre. 2018. Tese (Doutorado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10444.https://repositorio.ufscar.br/handle/ufscar/10444The phytoplankton community is composed of microalgae that support food chains in aquatic ecosystems. In these environments they are immersed in a complex matrix of organic and inorganic substances. In some situations, they are still subject to a range of anthropogenic wastes that end up entering aquatic environments, such as nanoparticles (NPs). Due to the unique characteristics of NPs, their use is intense, even if neither use nor disposal is regularized by competent governamental agencies. Toxicological studies on the effect of NPs on phytoplankton have controversial results. The toxicity of NPs is related to their reactivity, which depends on the size of the particles, formation of aggregates, availability of organic matter, among other factors. In this study, we first sought to understand the dynamics of NPs in algal cultures and followed investigating on the physiological effects of copper NPs (NPs-Cu) on two freshwater Chlorophyta (Chlorella sorokiniana and Ankistrodesmus densus) exposed to nominal NPs-Cu concentrations estimated for the environment (0.57 to 12.70 μg L-1) and above these (up to 635.0 μg L-1). We analyzed parameters related to growth, photosynthesis and intracellular biomolecules under controlled laboratory conditions at 72 h cultures. The results showed that the population growth rate was affected in both microalgae species. The intensity of the photosynthetic processes varied according to the microalgae, with C. sorokiniana being more resistant than A. densus that presented reductions in photosystem II quantum yield, electron transport rates and consequentely in the estimated gross primary productivity. The biochemical composition (proteins, carbohydrates and lipids) was affected in the presence of NPs-Cu in both microalgae, but at environmentally relevant concentrations of NPs-Cu only carbohydrates were affected. Protein synthesis was stimulated at the higher end of the NPs-Cu concentration range, suggesting the production of antioxidant proteins associated with cell detoxification. This study is a contribution to the knowledge of the toxicology of NPs to freshwater microalgae and their possible consequence in natural environment.A comunidade fitoplanctônica é composta por microalgas que suportam as cadeias alimentares em ecossistemas aquáticos. Nesses ambientes elas são imersas numa matriz complexa de substâncias orgânicas e inorgânicas. Em algumas situações, estão ainda sujeitas à uma gama de resíduos de origem antrópica que acabam entrando nos ambientes aquáticos, como é o caso das nanopartículas (NPs). Em virtude das características únicas das NPs é intenso seu uso, mesmo que nem o uso, nem o descarte sejam regularizados por órgão competente. Estudos toxicológicos sobre o efeito de NPs no fitoplâncton têm resultados controversos. A toxicidade das NPs está relacionada à sua reatividade, que depende do tamanho das partículas, formação de agregados, disponibilidade de matéria orgânica, dentre outros fatores. Neste estudo, buscamos primeiramente entender sobre a dinâmica das NPs no meio de cultura algal e depois investigamos os efeitos fisiológicos de NPs de cobre (NPs-Cu) em duas clorofíceas de água doce (Chlorella sorokiniana e Ankistrodesmus densus) expostas a concentrações nominais estimadas para o ambiente (0,57 a 12,70 µg L-1) e acima destas (até 635,0 µg L-1). Para isso analisamos parâmetros relacionados ao crescimento, fotossíntese e biomoléculas intracelulares sob condições controladas de laboratório em 72 h de exposição. Os resultados mostraram que o crescimento populacional foi afetado em ambas as espécies estudadas. Já a intensidade com que os processos fotossintéticos foram afetados variou conforme a microalga, C. sorokiniana foi mais resistente do que a A. densus, que mostrou reduções no rendimento quântico do fotossistema II e nas taxas de transporte de elétrons causando reduções significativas na produtividade primária desta microalga. A composição bioquímica (proteínas, carboidratos e lipídios) foi alterada na presença de NPs-Cu nas duas microalgas, mas em concentrações ambientalmente relevantes das NPs-Cu somente para carboidratos foi afetado. A síntese proteica foi estimulada nas maiores concentrações de NPs-Cu sugerindo a atuação de proteínas antioxidantes associadas à desintoxicação celular. Esse estudo é uma contribuição aos conhecimentos sobre a toxicologia de NPs em microalgas de água doce e sua possível consequência no ambiente natural.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Demanda social (DS) - 001porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Ecologia e Recursos Naturais - PPGERNUFSCarProdutividade primária brutaCarboidratosProteínasRendimento fotossintéticoTaxa de transporte de elétronsGross primary productivityProteinsCarbohydratesPhotosynthetic yieldElectron transport rateCIENCIAS BIOLOGICAS::BOTANICACIENCIAS BIOLOGICAS::ECOLOGIACIENCIAS BIOLOGICAS::FISIOLOGIACIENCIAS BIOLOGICASCIENCIAS BIOLOGICAS::FARMACOLOGIA::TOXICOLOGIAFisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobrePhysiology of Chlorella sorokiniana and Ankistrodesmus densus: effects and dynamics of copper nanoparticlesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisOnlineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARLICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/10444/3/license.txtae0398b6f8b235e40ad82cba6c50031dMD53ORIGINALBARRETO_Daniela_2018.pdfBARRETO_Daniela_2018.pdfapplication/pdf2016085https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/10444/4/BARRETO_Daniela_2018.pdf588d3b3cd8de582af099196d0dddf51eMD54TEXTBARRETO_Daniela_2018.pdf.txtBARRETO_Daniela_2018.pdf.txtExtracted texttext/plain169045https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/10444/5/BARRETO_Daniela_2018.pdf.txt50b64da327bd7c8851b89eeb991cae2fMD55THUMBNAILBARRETO_Daniela_2018.pdf.jpgBARRETO_Daniela_2018.pdf.jpgIM Thumbnailimage/jpeg7812https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/10444/6/BARRETO_Daniela_2018.pdf.jpg130a9257464470ea6689cbbf1897c26cMD56ufscar/104442020-02-27 13:50:36.949oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-05-25T12:56:26.651813Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
dc.title.alternative.eng.fl_str_mv |
Physiology of Chlorella sorokiniana and Ankistrodesmus densus: effects and dynamics of copper nanoparticles |
title |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
spellingShingle |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre Barreto, Daniela Mariano Produtividade primária bruta Carboidratos Proteínas Rendimento fotossintético Taxa de transporte de elétrons Gross primary productivity Proteins Carbohydrates Photosynthetic yield Electron transport rate CIENCIAS BIOLOGICAS::BOTANICA CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS::FISIOLOGIA CIENCIAS BIOLOGICAS CIENCIAS BIOLOGICAS::FARMACOLOGIA::TOXICOLOGIA |
title_short |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
title_full |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
title_fullStr |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
title_full_unstemmed |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
title_sort |
Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre |
author |
Barreto, Daniela Mariano |
author_facet |
Barreto, Daniela Mariano |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/0725648167756426 |
dc.contributor.author.fl_str_mv |
Barreto, Daniela Mariano |
dc.contributor.advisor1.fl_str_mv |
Lombardi, Ana Teresa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6737850858443813 |
dc.contributor.advisor-co1.fl_str_mv |
Tonietto, Alessandra Emanuele |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3056194008699912 |
contributor_str_mv |
Lombardi, Ana Teresa Tonietto, Alessandra Emanuele |
dc.subject.por.fl_str_mv |
Produtividade primária bruta Carboidratos Proteínas Rendimento fotossintético Taxa de transporte de elétrons |
topic |
Produtividade primária bruta Carboidratos Proteínas Rendimento fotossintético Taxa de transporte de elétrons Gross primary productivity Proteins Carbohydrates Photosynthetic yield Electron transport rate CIENCIAS BIOLOGICAS::BOTANICA CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS::FISIOLOGIA CIENCIAS BIOLOGICAS CIENCIAS BIOLOGICAS::FARMACOLOGIA::TOXICOLOGIA |
dc.subject.eng.fl_str_mv |
Gross primary productivity Proteins Carbohydrates Photosynthetic yield Electron transport rate |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BOTANICA CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS::FISIOLOGIA CIENCIAS BIOLOGICAS CIENCIAS BIOLOGICAS::FARMACOLOGIA::TOXICOLOGIA |
description |
The phytoplankton community is composed of microalgae that support food chains in aquatic ecosystems. In these environments they are immersed in a complex matrix of organic and inorganic substances. In some situations, they are still subject to a range of anthropogenic wastes that end up entering aquatic environments, such as nanoparticles (NPs). Due to the unique characteristics of NPs, their use is intense, even if neither use nor disposal is regularized by competent governamental agencies. Toxicological studies on the effect of NPs on phytoplankton have controversial results. The toxicity of NPs is related to their reactivity, which depends on the size of the particles, formation of aggregates, availability of organic matter, among other factors. In this study, we first sought to understand the dynamics of NPs in algal cultures and followed investigating on the physiological effects of copper NPs (NPs-Cu) on two freshwater Chlorophyta (Chlorella sorokiniana and Ankistrodesmus densus) exposed to nominal NPs-Cu concentrations estimated for the environment (0.57 to 12.70 μg L-1) and above these (up to 635.0 μg L-1). We analyzed parameters related to growth, photosynthesis and intracellular biomolecules under controlled laboratory conditions at 72 h cultures. The results showed that the population growth rate was affected in both microalgae species. The intensity of the photosynthetic processes varied according to the microalgae, with C. sorokiniana being more resistant than A. densus that presented reductions in photosystem II quantum yield, electron transport rates and consequentely in the estimated gross primary productivity. The biochemical composition (proteins, carbohydrates and lipids) was affected in the presence of NPs-Cu in both microalgae, but at environmentally relevant concentrations of NPs-Cu only carbohydrates were affected. Protein synthesis was stimulated at the higher end of the NPs-Cu concentration range, suggesting the production of antioxidant proteins associated with cell detoxification. This study is a contribution to the knowledge of the toxicology of NPs to freshwater microalgae and their possible consequence in natural environment. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-09-10T12:17:28Z |
dc.date.available.fl_str_mv |
2018-09-10T12:17:28Z |
dc.date.issued.fl_str_mv |
2018-08-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BARRETO, Daniela Mariano. Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre. 2018. Tese (Doutorado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10444. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/10444 |
identifier_str_mv |
BARRETO, Daniela Mariano. Fisiologia de Chlorella sorokiniana e Ankistrodesmus densus: efeitos e dinâmica de nanopartículas de cobre. 2018. Tese (Doutorado em Ecologia e Recursos Naturais) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10444. |
url |
https://repositorio.ufscar.br/handle/ufscar/10444 |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Ecologia e Recursos Naturais - PPGERN |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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