Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência e Engenharia de Materiais - PPGCEM
|
Departamento: |
Não Informado pela instituição
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/712 |
Resumo: | The aim of this study was to evaluate the properties of the surfaces of CP-Ti, Ti-6Al-4V, Ti-6Al-7Nb alloys modified via chemical treatments, as well as evaluating the behavior of human osteoblastic cells as function of these changes. For this purpose, samples of the three alloys were etched with hydrochloric acid or phosphoric acid, followed or not by alkaline treatment, and subsequently analyzed their morphology, roughness, effective area and contact angle (wettability). Scanning electron microscopy (SEM) was used to evaluate the morphology of surfaces and EDS for chemical compositions. Roughness and effective area measurements were performed with confocal laser microscope. A goniometer was used for the measurement of contact angle. Osteoblastic responses were evaluated by means of the technique of polymerase chain reaction with reverse transcription real-time (real-time PCR) to determine the gene expression of ALPL, COL1A1, COL3A1, SPP1, RUNX2 and SPARC. The results showed that the treatments significantly change the surface properties and induced the gene expression of human osteoblasts. All treatments caused change in the chemical composition of the sample surface. Acid treatments caused changes in actual topography and area, while alkaline treatments altered the surface morphology and increased hydrophilicity compared to the only abraded samples (controls). The gene expression was regulated differently in accordance with the surface treatment employed in the samples tested. Overall, treatment with alkaline treatment and phosphoric acid are more effective to control cell behavior. The alkali treatment and acid treatments together induce changes in the topography at micro, submicron and in some cases nanometric scales, which may amplify and accelerate the response of the cells on the materials. |
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Oliveira, Diego Pedreira deBolfarini, Claudemirohttp://lattes.cnpq.br/9231627080617037http://lattes.cnpq.br/83887908750285512016-06-02T19:10:18Z2013-11-222016-06-02T19:10:18Z2013-07-10OLIVEIRA, Diego Pedreira de. Surface modification of Ti-CP, Ti-6Al-4V e Ti-6Al-7Nb aiming osseointegration of implants: surface properties and osteoblastic responses. 2013. 147 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2013.https://repositorio.ufscar.br/handle/ufscar/712The aim of this study was to evaluate the properties of the surfaces of CP-Ti, Ti-6Al-4V, Ti-6Al-7Nb alloys modified via chemical treatments, as well as evaluating the behavior of human osteoblastic cells as function of these changes. For this purpose, samples of the three alloys were etched with hydrochloric acid or phosphoric acid, followed or not by alkaline treatment, and subsequently analyzed their morphology, roughness, effective area and contact angle (wettability). Scanning electron microscopy (SEM) was used to evaluate the morphology of surfaces and EDS for chemical compositions. Roughness and effective area measurements were performed with confocal laser microscope. A goniometer was used for the measurement of contact angle. Osteoblastic responses were evaluated by means of the technique of polymerase chain reaction with reverse transcription real-time (real-time PCR) to determine the gene expression of ALPL, COL1A1, COL3A1, SPP1, RUNX2 and SPARC. The results showed that the treatments significantly change the surface properties and induced the gene expression of human osteoblasts. All treatments caused change in the chemical composition of the sample surface. Acid treatments caused changes in actual topography and area, while alkaline treatments altered the surface morphology and increased hydrophilicity compared to the only abraded samples (controls). The gene expression was regulated differently in accordance with the surface treatment employed in the samples tested. Overall, treatment with alkaline treatment and phosphoric acid are more effective to control cell behavior. The alkali treatment and acid treatments together induce changes in the topography at micro, submicron and in some cases nanometric scales, which may amplify and accelerate the response of the cells on the materials.O objetivo deste trabalho foi avaliar as propriedades das superfícies de amostras de Ti-CP, Ti-6Al-4V, Ti-6Al-7Nb modificadas via tratamentos químicos, bem como avaliar o comportamento das células osteoblásticas humanas frente a essas modificações. Para tanto, amostras das três ligas foram atacadas com ácido clorídrico ou fosfórico, seguido ou não por tratamento alcalino, e posteriormente analisadas quanto à morfologia, rugosidade, área efetiva e ângulo de contato (molhabilidade). Microscopia eletrônica de varredura (MEV) foi utilizada para avaliar a morfologia das superfícies e EDS para obter as composições químicas. Medidas de rugosidade e área efetiva foram executadas com microscópio confocal a laser. O goniômetro foi o equipamento utilizado para as medidas de ângulo de contato. As respostas osteoblásticas foram avaliadas por meio da técnica de reação de polimerase em cadeia com transcrição reversa em tempo real (real time RT-PCR), para determinação das expressões gênicas de ALPL, COL1A1, COL3A1, SPP1, RUNX2 e SPARC. Os resultados demonstraram que os tratamentos provocaram alterações nas propriedades da superfície e induziram as expressões gênicas dos osteoblastos humanos. Todos os tratamentos provocaram alteração na composição química da superfície da amostra. Os tratamentos ácidos provocaram alterações de topografia e área efetiva, enquanto que os tratamentos alcalinos alteraram a morfologia da superfície e aumentaram a hidrofilicidade, todos em relação às amostras apenas lixadas (controles). As expressões genicas foram diferentemente reguladas de acordo com o tratamento empregado na superfície das amostras testadas. De forma geral, os tratamentos alcalinos e o ataque com ácido fosfórico são mais efetivos para controlar o comportamento celular. Os tratamentos ácidos e o tratamento alcalino induzem alterações de topografia nas escalas micro, submicro e, em alguns casos, nanométricas, fato que pode amplificar e acelerar as respostas das células sobre os materiais.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Ciência e Engenharia de Materiais - PPGCEMUFSCarBRMetaisLigas de titânioTratamento químicoBiocompatibilidadeExpressão gênicaENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICATratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticasSurface modification of Ti-CP, Ti-6Al-4V e Ti-6Al-7Nb aiming osseointegration of implants: surface properties and osteoblastic responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL5574.pdfapplication/pdf8900872https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/712/1/5574.pdf5422cf12edcd8bb4fb8c081fb5dacb77MD51TEXT5574.pdf.txt5574.pdf.txtExtracted texttext/plain0https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/712/2/5574.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52THUMBNAIL5574.pdf.jpg5574.pdf.jpgIM Thumbnailimage/jpeg6846https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/712/3/5574.pdf.jpg4cb9df10ef1a0ddb982af20da656dfacMD53ufscar/7122019-09-11 04:03:47.38oai:repositorio.ufscar.br:ufscar/712Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-05-25T12:43:38.070038Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
dc.title.alternative.eng.fl_str_mv |
Surface modification of Ti-CP, Ti-6Al-4V e Ti-6Al-7Nb aiming osseointegration of implants: surface properties and osteoblastic responses |
title |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
spellingShingle |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas Oliveira, Diego Pedreira de Metais Ligas de titânio Tratamento químico Biocompatibilidade Expressão gênica ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA |
title_short |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
title_full |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
title_fullStr |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
title_full_unstemmed |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
title_sort |
Tratamentos de superfícies em Ti-CP, Ti-6Al-4V e Ti-6Al- 7Nb visando a osseointegração de implantes: propriedades das superfícies e respostas osteoblásticas |
author |
Oliveira, Diego Pedreira de |
author_facet |
Oliveira, Diego Pedreira de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/8388790875028551 |
dc.contributor.author.fl_str_mv |
Oliveira, Diego Pedreira de |
dc.contributor.advisor1.fl_str_mv |
Bolfarini, Claudemiro |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9231627080617037 |
contributor_str_mv |
Bolfarini, Claudemiro |
dc.subject.por.fl_str_mv |
Metais Ligas de titânio Tratamento químico Biocompatibilidade Expressão gênica |
topic |
Metais Ligas de titânio Tratamento químico Biocompatibilidade Expressão gênica ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA |
description |
The aim of this study was to evaluate the properties of the surfaces of CP-Ti, Ti-6Al-4V, Ti-6Al-7Nb alloys modified via chemical treatments, as well as evaluating the behavior of human osteoblastic cells as function of these changes. For this purpose, samples of the three alloys were etched with hydrochloric acid or phosphoric acid, followed or not by alkaline treatment, and subsequently analyzed their morphology, roughness, effective area and contact angle (wettability). Scanning electron microscopy (SEM) was used to evaluate the morphology of surfaces and EDS for chemical compositions. Roughness and effective area measurements were performed with confocal laser microscope. A goniometer was used for the measurement of contact angle. Osteoblastic responses were evaluated by means of the technique of polymerase chain reaction with reverse transcription real-time (real-time PCR) to determine the gene expression of ALPL, COL1A1, COL3A1, SPP1, RUNX2 and SPARC. The results showed that the treatments significantly change the surface properties and induced the gene expression of human osteoblasts. All treatments caused change in the chemical composition of the sample surface. Acid treatments caused changes in actual topography and area, while alkaline treatments altered the surface morphology and increased hydrophilicity compared to the only abraded samples (controls). The gene expression was regulated differently in accordance with the surface treatment employed in the samples tested. Overall, treatment with alkaline treatment and phosphoric acid are more effective to control cell behavior. The alkali treatment and acid treatments together induce changes in the topography at micro, submicron and in some cases nanometric scales, which may amplify and accelerate the response of the cells on the materials. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-11-22 2016-06-02T19:10:18Z |
dc.date.issued.fl_str_mv |
2013-07-10 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:10:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Diego Pedreira de. Surface modification of Ti-CP, Ti-6Al-4V e Ti-6Al-7Nb aiming osseointegration of implants: surface properties and osteoblastic responses. 2013. 147 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/712 |
identifier_str_mv |
OLIVEIRA, Diego Pedreira de. Surface modification of Ti-CP, Ti-6Al-4V e Ti-6Al-7Nb aiming osseointegration of implants: surface properties and osteoblastic responses. 2013. 147 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2013. |
url |
https://repositorio.ufscar.br/handle/ufscar/712 |
dc.language.iso.fl_str_mv |
por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciência e Engenharia de Materiais - PPGCEM |
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UFSCar |
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BR |
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Universidade Federal de São Carlos |
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