Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia
Ano de defesa: | 2019 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química - PPGQ
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/11843 |
Resumo: | The diversity of the bacterial community associated with the Podocarpus Macrophyllus plant has been systematically studied in order to determine the genera of bacteria that are commonly associated with this plant, as well as the composition of the community bacterial changes due to regional and seasonal conditions. It was included that genus Bacillus is the main associated with the host plant, being isolated in all regions and seasons, and in all tissues used in the procedures of isolation. Within this genus, the species Bacillus pumilus was largely isolated in all studied conditions. Additionally, the biotechnological potential of the main bacteria associated with the plant was evaluated by the studying the production of fixed and volatile compounds, as well as the interaction with endophytic fungi associated with the same plant. These bacteria are skillful producing lipopeptides, surfactin and iturin were identified and isolated from all strains, fengycins were isolated from LaBioMMi 1180 and 1202 strains that correspond to Bacillus subtilis. Finally, the plantazolicin was isolated from LaBioMMi 1137, which corresponds to Bacillus pumilus, and such metabolites are the main responsible for the inhibitory activity of these bactéria, when cultivated with endophytic fungi Isolated from the same plant. With regard to volatile compounds, all strains demonstrate great ability to produce nitrogenous compounds, such as pyrazine, and oxygenated compounds as short chain ketones. Bacillus spp. demonstrates great potential for biotechnological application for bioremmenation and biodegradation of polymers. When cultivated in medium enriched with Fe3+, LaBioMMi 1141 demonstrated the ability to absorve much of the heavy metal content depending on the condition in which it was cultivated. The results for the biosorption of Cu2+ and the composition of Fe3+ together with Cu2+ showed no satisfactory results in comparison with Fe3+ alone. On the other hand, LaBioMMi 1180 has been shown to be able to degradate several polymers, when these are the unique carbon source in the medium, thus demonstrating the great potential of these endophytic microorganisms. |
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Pereira, Thieres Magaive CostaRodrigues Filho, Edsonhttp://lattes.cnpq.br/3667941735597178http://lattes.cnpq.br/37230510137161792019-09-17T13:24:20Z2019-09-17T13:24:20Z2019-06-05PEREIRA, Thieres Magaive Costa. Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia. 2019. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11843.https://repositorio.ufscar.br/handle/ufscar/11843The diversity of the bacterial community associated with the Podocarpus Macrophyllus plant has been systematically studied in order to determine the genera of bacteria that are commonly associated with this plant, as well as the composition of the community bacterial changes due to regional and seasonal conditions. It was included that genus Bacillus is the main associated with the host plant, being isolated in all regions and seasons, and in all tissues used in the procedures of isolation. Within this genus, the species Bacillus pumilus was largely isolated in all studied conditions. Additionally, the biotechnological potential of the main bacteria associated with the plant was evaluated by the studying the production of fixed and volatile compounds, as well as the interaction with endophytic fungi associated with the same plant. These bacteria are skillful producing lipopeptides, surfactin and iturin were identified and isolated from all strains, fengycins were isolated from LaBioMMi 1180 and 1202 strains that correspond to Bacillus subtilis. Finally, the plantazolicin was isolated from LaBioMMi 1137, which corresponds to Bacillus pumilus, and such metabolites are the main responsible for the inhibitory activity of these bactéria, when cultivated with endophytic fungi Isolated from the same plant. With regard to volatile compounds, all strains demonstrate great ability to produce nitrogenous compounds, such as pyrazine, and oxygenated compounds as short chain ketones. Bacillus spp. demonstrates great potential for biotechnological application for bioremmenation and biodegradation of polymers. When cultivated in medium enriched with Fe3+, LaBioMMi 1141 demonstrated the ability to absorve much of the heavy metal content depending on the condition in which it was cultivated. The results for the biosorption of Cu2+ and the composition of Fe3+ together with Cu2+ showed no satisfactory results in comparison with Fe3+ alone. On the other hand, LaBioMMi 1180 has been shown to be able to degradate several polymers, when these are the unique carbon source in the medium, thus demonstrating the great potential of these endophytic microorganisms.A diversidade da comunidade bacteriana associada à planta Podocarpus macrophyllus foi estudada sistematicamente a fim de se determinar os gêneros de bactérias que comumente estão associadas a essa planta com maior frequência, bem como a composição da comunidade bacteriana se altera devido a condições regionais e sazonais. Constou-se que gênero Bacillus é principal associado à planta hospedeira, sendo isolado em todas as regiões e estações do ano, e em todos os tecidos utilizados nos procedimentos de isolamento. Dentro desse gênero, a espécie Bacillus pumilus foi majoritariamente isolada em todas as condições de estudo. Adicionalmente, o potencial biotecnológico das principais bactérias associadas à planta foi avaliado através do estudo da produção de compostos fixos e voláteis, por essas bactérias, bem com a interação com fungos endofíticos associados a mesma planta. Essas bactérias são hábeis produtoras de lipopeptídeos, sendo que moléculas de surfactina, iturina foram identificadas e isoladas de todas as cepas, moléculas de fengicina foram isoladas das cepas LaBioMMi 1180 e 1202 que correspondem a espécie Bacillus subtilis, por fim a molécula de plantazolicina foi isolada a partir do LaBioMMi 1137 que corresponde ao Bacillus pumilus. Tais metabólitos são os principais responsáveis pela atividade inibitória dessas bactérias quando cultivadas com fungos endofíticos isolados da mesma planta. Com relação aos compostos voláteis, todas as cepas demonstraram grande habilidade para produção de compostos nitrogenados como pirazina e compostos oxigenados como cetonas de cadeia curta. Os Bacillus spp. demonstram grande potencial de aplicação biotecnológica em biorremediação e biodegradação de polímeros. Quando cultivado em meios enriquecidos com Fe3+, o LaBioMMi 1141 demonstrou a capacidade de biossorver grande parte do conteúdo de metal pesado dependendo da condição em que foi cultivado. Os resultados para biossorção de Cu2+ e da composição de Fe3+ juntamente com Cu2+, não foram semelhantes aos observados para o Fe3+ isoladamente. Em contrapartida, o LaBioMMi 1180 demonstrou ser capaz de biodegradar diversos polímeros, quando esses são a única fonte de carbono do meio.Não recebi financiamentoporUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessPodocarpus macrophyllusBactérias endofíticasMALDI TOF/MSSurfactinaIturinaFengicinaPlantazolicinaCompostos voláteisCo-cultivoBiorremediaçãoBiodegradaçãoEndophyte bacteriaSurfactinVolatile compoundsCo-cultureeBioremediationBiodegradationCIENCIAS EXATAS E DA TERRA::QUIMICAEstudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologiaA study of the bacterial community associated with Podocarpus macrophyllus: interactions in the microbiota, fixed and volatile compounds, and the potential for application in biotechnologicalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisOnlinereponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL19082019_TMCP_tese_final.pdf19082019_TMCP_tese_final.pdfapplication/pdf11746217https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/3/19082019_TMCP_tese_final.pdf0a1bf38fe0b6a924fb0dfa0265133178MD53Thieres_1.pdfThieres_1.pdfCarta comprovanteapplication/pdf258555https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/4/Thieres_1.pdfa32806ae6c233d6c3a82054b92e61f87MD54CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/5/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD55TEXT19082019_TMCP_tese_final.pdf.txt19082019_TMCP_tese_final.pdf.txtExtracted texttext/plain399919https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/6/19082019_TMCP_tese_final.pdf.txt15ccafb86155e4f6028ad82ed0e1f20dMD56Thieres_1.pdf.txtThieres_1.pdf.txtExtracted texttext/plain22https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/8/Thieres_1.pdf.txtcc4a1be4ad7e47ba4fe5327259a9f389MD58THUMBNAIL19082019_TMCP_tese_final.pdf.jpg19082019_TMCP_tese_final.pdf.jpgIM Thumbnailimage/jpeg10011https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/7/19082019_TMCP_tese_final.pdf.jpg86162679fddc85f00dd841b95bf73405MD57Thieres_1.pdf.jpgThieres_1.pdf.jpgIM Thumbnailimage/jpeg13914https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/11843/9/Thieres_1.pdf.jpg98d267b8db1e23658bcc9e0a7e84a61fMD59ufscar/118432019-09-18 06:15:26.788oai:repositorio.ufscar.br:ufscar/11843Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-05-25T12:58:09.104568Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
dc.title.alternative.eng.fl_str_mv |
A study of the bacterial community associated with Podocarpus macrophyllus: interactions in the microbiota, fixed and volatile compounds, and the potential for application in biotechnological |
title |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
spellingShingle |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia Pereira, Thieres Magaive Costa Podocarpus macrophyllus Bactérias endofíticas MALDI TOF/MS Surfactina Iturina Fengicina Plantazolicina Compostos voláteis Co-cultivo Biorremediação Biodegradação Endophyte bacteria Surfactin Volatile compounds Co-culturee Bioremediation Biodegradation CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
title_full |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
title_fullStr |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
title_full_unstemmed |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
title_sort |
Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia |
author |
Pereira, Thieres Magaive Costa |
author_facet |
Pereira, Thieres Magaive Costa |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/3723051013716179 |
dc.contributor.author.fl_str_mv |
Pereira, Thieres Magaive Costa |
dc.contributor.advisor1.fl_str_mv |
Rodrigues Filho, Edson |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3667941735597178 |
contributor_str_mv |
Rodrigues Filho, Edson |
dc.subject.por.fl_str_mv |
Podocarpus macrophyllus Bactérias endofíticas MALDI TOF/MS Surfactina Iturina Fengicina Plantazolicina Compostos voláteis Co-cultivo Biorremediação Biodegradação |
topic |
Podocarpus macrophyllus Bactérias endofíticas MALDI TOF/MS Surfactina Iturina Fengicina Plantazolicina Compostos voláteis Co-cultivo Biorremediação Biodegradação Endophyte bacteria Surfactin Volatile compounds Co-culturee Bioremediation Biodegradation CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Endophyte bacteria Surfactin Volatile compounds Co-culturee Bioremediation Biodegradation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The diversity of the bacterial community associated with the Podocarpus Macrophyllus plant has been systematically studied in order to determine the genera of bacteria that are commonly associated with this plant, as well as the composition of the community bacterial changes due to regional and seasonal conditions. It was included that genus Bacillus is the main associated with the host plant, being isolated in all regions and seasons, and in all tissues used in the procedures of isolation. Within this genus, the species Bacillus pumilus was largely isolated in all studied conditions. Additionally, the biotechnological potential of the main bacteria associated with the plant was evaluated by the studying the production of fixed and volatile compounds, as well as the interaction with endophytic fungi associated with the same plant. These bacteria are skillful producing lipopeptides, surfactin and iturin were identified and isolated from all strains, fengycins were isolated from LaBioMMi 1180 and 1202 strains that correspond to Bacillus subtilis. Finally, the plantazolicin was isolated from LaBioMMi 1137, which corresponds to Bacillus pumilus, and such metabolites are the main responsible for the inhibitory activity of these bactéria, when cultivated with endophytic fungi Isolated from the same plant. With regard to volatile compounds, all strains demonstrate great ability to produce nitrogenous compounds, such as pyrazine, and oxygenated compounds as short chain ketones. Bacillus spp. demonstrates great potential for biotechnological application for bioremmenation and biodegradation of polymers. When cultivated in medium enriched with Fe3+, LaBioMMi 1141 demonstrated the ability to absorve much of the heavy metal content depending on the condition in which it was cultivated. The results for the biosorption of Cu2+ and the composition of Fe3+ together with Cu2+ showed no satisfactory results in comparison with Fe3+ alone. On the other hand, LaBioMMi 1180 has been shown to be able to degradate several polymers, when these are the unique carbon source in the medium, thus demonstrating the great potential of these endophytic microorganisms. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-09-17T13:24:20Z |
dc.date.available.fl_str_mv |
2019-09-17T13:24:20Z |
dc.date.issued.fl_str_mv |
2019-06-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Thieres Magaive Costa. Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia. 2019. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11843. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/11843 |
identifier_str_mv |
PEREIRA, Thieres Magaive Costa. Estudo da comunidade bacteriana associada a Podocarpus macrophyllus: interações na microbiota, compostos fixos e voláteis, e do potencial de aplicação na biotecnologia. 2019. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11843. |
url |
https://repositorio.ufscar.br/handle/ufscar/11843 |
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por |
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por |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Química - PPGQ |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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