Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Souza, Markus Vinicius Campos
Orientador(a): Perez, Sergio Eduardo de Andrade lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Carlos
Câmpus São Carlos
Programa de Pós-Graduação: Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Osteoporose
Treinamento resistido
Ovariectomia
Reposição hormonal
Matrix metaloprotease 2 (MMP-2)
Palavras-chave em Inglês:
Osteoporosis
Resistance training
Ovariectomy
Hormone replacement
Metalloproteinase-2 (MMP-2)
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS
Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/8146
Resumo: Purpose: The aim of the present study was to investigate the influence of resistance training (RT) and hormone replacement (HR) on MMP-2 activity, biomechanical and physical properties bone of ovariectomized (OVX) rats. Methods: Sprague-Dawley female rats were grouped into six experimental groups (n = 11 per group): shamoperated sedentary (SHAM Sed), ovariectomized sedentary (OVX Sed), sham-operated resistance training (SHAM RT), ovariectomized resistance training (OVX RT),(OVX Sed-HR), and OVX RT-HR. HR groups received implanted silastic capsules with a 5% solution of 17β-estradiol (50 mg 17β-estradiol/ml of sunflower oil). In a 12-week RT period (27 sessions; 4–9 climbs) the animals climbed a 1.1 m vertical ladder with weights attached to their tails. Biomechanical and physical bone analyses were performed using a universal testing machine, and MMP-2 activity analysis was done by zymography. Results: Bone density and bone mineral content was higher in the RT and HR groups. The MMP-2 activity was higher in the RT and HR groups. The biomechanical analysis (stiffness, fracture load and maximum load) demonstrated better bone tissue quality in the RT associated with HR. Conclusion: The RT alone as well as when it is associated with HR was efficient in increasing MMP-2 activity, biomechanical and biophysical properties bone of ovariectomized rats.
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spelling Souza, Markus Vinicius CamposPerez, Sergio Eduardo de Andradehttp://lattes.cnpq.br/9408953831211309http://lattes.cnpq.br/2989329210364477b8b3a561-9ace-4abc-b9f4-b0191b06ef462016-10-21T13:45:24Z2016-10-21T13:45:24Z2016-09-16SOUZA, Markus Vinicius Campos. Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas. 2016. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/20.500.14289/8146.https://repositorio.ufscar.br/handle/20.500.14289/8146Purpose: The aim of the present study was to investigate the influence of resistance training (RT) and hormone replacement (HR) on MMP-2 activity, biomechanical and physical properties bone of ovariectomized (OVX) rats. Methods: Sprague-Dawley female rats were grouped into six experimental groups (n = 11 per group): shamoperated sedentary (SHAM Sed), ovariectomized sedentary (OVX Sed), sham-operated resistance training (SHAM RT), ovariectomized resistance training (OVX RT),(OVX Sed-HR), and OVX RT-HR. HR groups received implanted silastic capsules with a 5% solution of 17β-estradiol (50 mg 17β-estradiol/ml of sunflower oil). In a 12-week RT period (27 sessions; 4–9 climbs) the animals climbed a 1.1 m vertical ladder with weights attached to their tails. Biomechanical and physical bone analyses were performed using a universal testing machine, and MMP-2 activity analysis was done by zymography. Results: Bone density and bone mineral content was higher in the RT and HR groups. The MMP-2 activity was higher in the RT and HR groups. The biomechanical analysis (stiffness, fracture load and maximum load) demonstrated better bone tissue quality in the RT associated with HR. Conclusion: The RT alone as well as when it is associated with HR was efficient in increasing MMP-2 activity, biomechanical and biophysical properties bone of ovariectomized rats.Objetivo: o objetivo do presente estudo foi investigar a influência do treinamento resistido (TR) e reposição hormonal (RH) na atividade das metalloproteinases 2 (MMP- 2), nas propriedades biomecânicas e biofísicas do osso de ratas ovariectomizadas (OVX). Métodos: fêmeas albinas foram agrupadas em seis grupos (11 animais por grupo): Sham Sedentário (SHAM Sed); Ovariectomizado Sedentário (OVX Sed); Sham Operado Treinamento Resistido (SHAM TR); Ovariectomizado Treinamento Resistido (OVX TR); Ovariectomizado Sedentário com Reposição Hormonal (OVX Sed-RH) e Ovariectomizado Treinamento Resistido com Reposição Hormonal (OVX TR-RH). Os animais dos grupos que receberam RH tiveram capsulas de silastic implantadas com uma solução de óleo de girassol com 180μg de 17β-estradiol/mL. Os animais foram submetidos ao TR por 12 semanas (3 vezes por semana, onde realizavam de 4-9 escaladas), o treinamento acontecia em uma escada vertical de 1,1m com os pesos fixados em sua cauda. As análises biomecânicas e biofísicas foram realizadas utilizando a máquina de teste universal e a atividade da MMP-2 por zimografia. Resultados: a densidade óssea e o conteúdo mineral ósseo foram maior nos grupos TR e RH. A atividade da MMP-2 também foi maior nos grupos que foram submetidos ao TR e RH. A análise biomecânica (rigidez, carga no ponto de fratura e carga máxima) utilizada para demonstrar a qualidade do osso foi maios no grupo TR com associação do RH. Conclusão: nossos resultados indicam que o TR e RH foram eficientes em aumentar a atividade da MMP-2, as propriedades biomecânicas e biofísicas dos ossos de ratas ovariectomizadas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)porUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarOsteoporoseTreinamento resistidoOvariectomiaReposição hormonalMatrix metaloprotease 2 (MMP-2)OsteoporosisResistance trainingOvariectomyHormone replacementMetalloproteinase-2 (MMP-2)CIENCIAS BIOLOGICASTreinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisOnline60060094c0c56d-5b6c-4622-8f35-ef5d57c5c02cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTeseMVCS.pdfTeseMVCS.pdfapplication/pdf1443535https://repositorio.ufscar.br/bitstreams/6ea2b729-e5aa-4ac6-bd37-5648ef539901/download00790f3f7d8d3a096d595944eba6c76dMD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstreams/8e2f1603-6261-43d3-89ce-80db7e0fb758/downloadae0398b6f8b235e40ad82cba6c50031dMD52falseAnonymousREADTEXTTeseMVCS.pdf.txtTeseMVCS.pdf.txtExtracted texttext/plain111652https://repositorio.ufscar.br/bitstreams/d414ba54-f5e1-4f9c-aac9-741cf14ecc5d/download65de7a1b4f6b22e050f8eef95eeb401dMD55falseAnonymousREADTHUMBNAILTeseMVCS.pdf.jpgTeseMVCS.pdf.jpgIM Thumbnailimage/jpeg3297https://repositorio.ufscar.br/bitstreams/30d69673-22c1-41ff-9a61-6effc070d64d/downloadf75b8206325e3808ee8a0302222409c0MD56falseAnonymousREAD20.500.14289/81462025-02-05 18:54:20.933Acesso abertoopen.accessoai:repositorio.ufscar.br:20.500.14289/8146https://repositorio.ufscar.brRepositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestrepositorio.sibi@ufscar.bropendoar:43222025-02-05T21:54:20Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)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
dc.title.por.fl_str_mv Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
title Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
spellingShingle Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
Souza, Markus Vinicius Campos
Osteoporose
Treinamento resistido
Ovariectomia
Reposição hormonal
Matrix metaloprotease 2 (MMP-2)
Osteoporosis
Resistance training
Ovariectomy
Hormone replacement
Metalloproteinase-2 (MMP-2)
CIENCIAS BIOLOGICAS
title_short Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
title_full Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
title_fullStr Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
title_full_unstemmed Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
title_sort Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas
author Souza, Markus Vinicius Campos
author_facet Souza, Markus Vinicius Campos
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/2989329210364477
dc.contributor.author.fl_str_mv Souza, Markus Vinicius Campos
dc.contributor.advisor1.fl_str_mv Perez, Sergio Eduardo de Andrade
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9408953831211309
dc.contributor.authorID.fl_str_mv b8b3a561-9ace-4abc-b9f4-b0191b06ef46
contributor_str_mv Perez, Sergio Eduardo de Andrade
dc.subject.por.fl_str_mv Osteoporose
Treinamento resistido
Ovariectomia
Reposição hormonal
Matrix metaloprotease 2 (MMP-2)
topic Osteoporose
Treinamento resistido
Ovariectomia
Reposição hormonal
Matrix metaloprotease 2 (MMP-2)
Osteoporosis
Resistance training
Ovariectomy
Hormone replacement
Metalloproteinase-2 (MMP-2)
CIENCIAS BIOLOGICAS
dc.subject.eng.fl_str_mv Osteoporosis
Resistance training
Ovariectomy
Hormone replacement
Metalloproteinase-2 (MMP-2)
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS
description Purpose: The aim of the present study was to investigate the influence of resistance training (RT) and hormone replacement (HR) on MMP-2 activity, biomechanical and physical properties bone of ovariectomized (OVX) rats. Methods: Sprague-Dawley female rats were grouped into six experimental groups (n = 11 per group): shamoperated sedentary (SHAM Sed), ovariectomized sedentary (OVX Sed), sham-operated resistance training (SHAM RT), ovariectomized resistance training (OVX RT),(OVX Sed-HR), and OVX RT-HR. HR groups received implanted silastic capsules with a 5% solution of 17β-estradiol (50 mg 17β-estradiol/ml of sunflower oil). In a 12-week RT period (27 sessions; 4–9 climbs) the animals climbed a 1.1 m vertical ladder with weights attached to their tails. Biomechanical and physical bone analyses were performed using a universal testing machine, and MMP-2 activity analysis was done by zymography. Results: Bone density and bone mineral content was higher in the RT and HR groups. The MMP-2 activity was higher in the RT and HR groups. The biomechanical analysis (stiffness, fracture load and maximum load) demonstrated better bone tissue quality in the RT associated with HR. Conclusion: The RT alone as well as when it is associated with HR was efficient in increasing MMP-2 activity, biomechanical and biophysical properties bone of ovariectomized rats.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-10-21T13:45:24Z
dc.date.available.fl_str_mv 2016-10-21T13:45:24Z
dc.date.issued.fl_str_mv 2016-09-16
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv SOUZA, Markus Vinicius Campos. Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas. 2016. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/20.500.14289/8146.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/20.500.14289/8146
identifier_str_mv SOUZA, Markus Vinicius Campos. Treinamento resistido e reposição hormonal aumentam a atividade das metaloproteinases-2 e a qualidade de osso de ratas ovariectomizadas. 2016. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/20.500.14289/8146.
url https://repositorio.ufscar.br/handle/20.500.14289/8146
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dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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