Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.].
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química - PPGQ
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/ufscar/14309 |
Resumo: | The soybean (Glycine max) is a legume species originally from China, belonging to the Fabaceae family. This crop is exposed throughout its cycle to attack by different species of insect pests, which can affect the plant development and, consequently, its productivity. Some studies classify flavonoids as relevant constituents in the defense of soybean against injury to herbivores. Despite the potential role of flavonoids against herbivores, little is known about the variability and spatial and temporal allocation of these defenses in soybean leaves and throughout its developmental stages. Therefore, this work aims to investigate the chemical profile of a commercial soybean cultivar, comparing the flavonoid profiles from different leaves over different developmental stages. Conventional soybean seeds BRS 284 were germinated and grown in a greenhouse. Samples of old, young and immature leaves from emergency stage (VC), cotyledon stage (VE), vegetative stages 1, 2, 3, 4 and 5 (V1 to V5), and reproductive stages 1 and 2 (R1 and R2) were collected and extracted in 1:1 (v/v) methanol: water, and the extracts were analyzed by LC- MS. The main flavonoids of soybean leaves were identified by mass spectra and relatively quantified. During the developmental stages, higher levels of kaempferol mono, di and isomeric triglycerides found in immature and young leaves were found. The initial stages, emergency vegetative and coethylene vegetative, showed higher concentrations of isoflavones such as daidzein, genistein, glycitein and their glycoconjugates, which are more abundant in old leaves. The chemical profile and allocation dynamics of the studied flavonoids provide information on the different strategies used by the plant to allocate its resources, demonstrating that leaves of different ages differ in their composition, possibly according to their physiological and ecological context. Therefore, evidence of the role of constitutive flavonoids in the defense mechanism of soy will help in the development of methods that can contribute to agricultural productivity, adaptability and resistance to various stressors in a more sustainable way. |
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Costa, Josicleia OliveiraWouters, Felipe Christoffhttp://lattes.cnpq.br/4392349445398645http://lattes.cnpq.br/946998596877762604ea7d90-690d-4eca-a06c-7ec7f748eea12021-05-26T11:47:57Z2021-05-26T11:47:57Z2021-03-11COSTA, Josicleia Oliveira. Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.].. 2021. Dissertação (Mestrado em Química) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/14309.https://repositorio.ufscar.br/handle/ufscar/14309The soybean (Glycine max) is a legume species originally from China, belonging to the Fabaceae family. This crop is exposed throughout its cycle to attack by different species of insect pests, which can affect the plant development and, consequently, its productivity. Some studies classify flavonoids as relevant constituents in the defense of soybean against injury to herbivores. Despite the potential role of flavonoids against herbivores, little is known about the variability and spatial and temporal allocation of these defenses in soybean leaves and throughout its developmental stages. Therefore, this work aims to investigate the chemical profile of a commercial soybean cultivar, comparing the flavonoid profiles from different leaves over different developmental stages. Conventional soybean seeds BRS 284 were germinated and grown in a greenhouse. Samples of old, young and immature leaves from emergency stage (VC), cotyledon stage (VE), vegetative stages 1, 2, 3, 4 and 5 (V1 to V5), and reproductive stages 1 and 2 (R1 and R2) were collected and extracted in 1:1 (v/v) methanol: water, and the extracts were analyzed by LC- MS. The main flavonoids of soybean leaves were identified by mass spectra and relatively quantified. During the developmental stages, higher levels of kaempferol mono, di and isomeric triglycerides found in immature and young leaves were found. The initial stages, emergency vegetative and coethylene vegetative, showed higher concentrations of isoflavones such as daidzein, genistein, glycitein and their glycoconjugates, which are more abundant in old leaves. The chemical profile and allocation dynamics of the studied flavonoids provide information on the different strategies used by the plant to allocate its resources, demonstrating that leaves of different ages differ in their composition, possibly according to their physiological and ecological context. Therefore, evidence of the role of constitutive flavonoids in the defense mechanism of soy will help in the development of methods that can contribute to agricultural productivity, adaptability and resistance to various stressors in a more sustainable way.A soja (Glycine max) é uma espécie de leguminosa originária da China, pertencente à família Fabaceae. A cultura da soja está exposta durante todo o seu ciclo a diferentes fatores como ataque de insetos-praga e as condições ambientais, que podem afetar o desenvolvimento da planta e, consequentemente, sua produtividade. Alguns estudos classificam os flavonoides como constituintes relevantes na defesa da soja contra injúria de herbívoros. Apesar do potencial papel de flavonoides contra herbívoros, pouco se sabe sobre a variabilidade e alocação espacial e temporal dessas defesas nas folhas de soja e ao longo dos estágios de desenvolvimento. Diante do exposto, este trabalho visa investigar o perfil químico de um cultivar comercial de soja, comparando o perfil de flavonoides de diferentes folhas ao longo de diferentes estágios de desenvolvimento. As sementes de soja convencional BRS 284, foram germinadas e mantidas em casa de vegetação. Amostras de folhas velhas, jovens e imaturas dos estágios vegetativo emergencial (VE), vegetativo cotiledonar (VC), vegetativos 1, 2, 3, 4 e 5 (V1 a V5), e reprodutivos 1 e 2 (R1 e R2) foram coletadas e extraídas em metanol:água 1:1 (v/v) e os extratos foram analisados por Cromatografia Líquida acoplada à Espectrometria de Massas (LC-MS). Os principais flavonoides das folhas de soja foram identificados pelos espectros de massas e quantificados de forma relativa. Durante os estágios de desenvolvimento, foram encontrados níveis mais elevados de mono, di e triglicosídeos isoméricos de kaempferol alocados nas folhas imaturas e jovens. Os estágios iniciais, VE e VC, tiveram uma maior concentração de isoflavonas como daidzeína, genisteína e seus glicoconjugados, sendo estes mais abundantes nas folhas velhas. O perfil químico e a dinâmica de alocação dos flavonoides estudados fornecem informações sobre as diferentes estratégias empregadas pela planta para alocar seus recursos e mostram que folhas de diferentes idades possuem diferenças em suas composições, possivelmente de acordo com seu contexto fisiológico e ecológico. Portanto, evidências do papel dos flavonoides constitutivos no mecanismo de defesa da soja ajudarão no desenvolvimento de métodos que podem contribuir para a produtividade agrícola, adaptabilidade e resistência a diversos agentes estressores de maneira mais sustentável.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: código de financiamento - 001porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessSojaFlavonoidesIsoflavonasDefesasMetabólitos secundáriosSoybeanFlavonoidsIsoflavonesDefensesSecondary metabolitesCIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAISPerfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.].Chemical profile and spatial and temporal dynamics of soybean flavonoids [Glycine max (L.) Merr.].info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6006004dc1e857-50fd-410f-8d79-bd44db1224adreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDISSERTAÇÃO OFICIAL.pdfDISSERTAÇÃO OFICIAL.pdfDissertação oficialapplication/pdf2802185https://repositorio.ufscar.br/bitstream/ufscar/14309/1/DISSERTA%c3%87%c3%83O%20OFICIAL.pdfdd4869a5fe03cb9a1033a3299d13c342MD51comprovante de homologacao.pdfcomprovante de homologacao.pdfCarta comprovante da versão final da dissertaçãoapplication/pdf174538https://repositorio.ufscar.br/bitstream/ufscar/14309/2/comprovante%20de%20homologacao.pdfd730b0a689f0c0f76e31822532055f0fMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/14309/4/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD54TEXTDISSERTAÇÃO OFICIAL.pdf.txtDISSERTAÇÃO OFICIAL.pdf.txtExtracted texttext/plain100693https://repositorio.ufscar.br/bitstream/ufscar/14309/5/DISSERTA%c3%87%c3%83O%20OFICIAL.pdf.txt13607a8eed1434ef62c2b95b4a97a4f3MD55comprovante de homologacao.pdf.txtcomprovante de homologacao.pdf.txtExtracted texttext/plain1423https://repositorio.ufscar.br/bitstream/ufscar/14309/7/comprovante%20de%20homologacao.pdf.txt3eca7383b3ca642d34f46f78fa7acc68MD57THUMBNAILDISSERTAÇÃO OFICIAL.pdf.jpgDISSERTAÇÃO OFICIAL.pdf.jpgIM Thumbnailimage/jpeg10004https://repositorio.ufscar.br/bitstream/ufscar/14309/6/DISSERTA%c3%87%c3%83O%20OFICIAL.pdf.jpg329b111c6564a45d96934739ea62cd06MD56comprovante de homologacao.pdf.jpgcomprovante de homologacao.pdf.jpgIM Thumbnailimage/jpeg12398https://repositorio.ufscar.br/bitstream/ufscar/14309/8/comprovante%20de%20homologacao.pdf.jpgc024b6f91983748a8b16f67e1a85c5bfMD58ufscar/143092023-09-18 18:32:11.159oai:repositorio.ufscar.br:ufscar/14309Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:11Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
dc.title.alternative.eng.fl_str_mv |
Chemical profile and spatial and temporal dynamics of soybean flavonoids [Glycine max (L.) Merr.]. |
title |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
spellingShingle |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. Costa, Josicleia Oliveira Soja Flavonoides Isoflavonas Defesas Metabólitos secundários Soybean Flavonoids Isoflavones Defenses Secondary metabolites CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
title_short |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
title_full |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
title_fullStr |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
title_full_unstemmed |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
title_sort |
Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.]. |
author |
Costa, Josicleia Oliveira |
author_facet |
Costa, Josicleia Oliveira |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/9469985968777626 |
dc.contributor.author.fl_str_mv |
Costa, Josicleia Oliveira |
dc.contributor.advisor1.fl_str_mv |
Wouters, Felipe Christoff |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4392349445398645 |
dc.contributor.authorID.fl_str_mv |
04ea7d90-690d-4eca-a06c-7ec7f748eea1 |
contributor_str_mv |
Wouters, Felipe Christoff |
dc.subject.por.fl_str_mv |
Soja Flavonoides Isoflavonas Defesas Metabólitos secundários |
topic |
Soja Flavonoides Isoflavonas Defesas Metabólitos secundários Soybean Flavonoids Isoflavones Defenses Secondary metabolites CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
dc.subject.eng.fl_str_mv |
Soybean Flavonoids Isoflavones Defenses Secondary metabolites |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
description |
The soybean (Glycine max) is a legume species originally from China, belonging to the Fabaceae family. This crop is exposed throughout its cycle to attack by different species of insect pests, which can affect the plant development and, consequently, its productivity. Some studies classify flavonoids as relevant constituents in the defense of soybean against injury to herbivores. Despite the potential role of flavonoids against herbivores, little is known about the variability and spatial and temporal allocation of these defenses in soybean leaves and throughout its developmental stages. Therefore, this work aims to investigate the chemical profile of a commercial soybean cultivar, comparing the flavonoid profiles from different leaves over different developmental stages. Conventional soybean seeds BRS 284 were germinated and grown in a greenhouse. Samples of old, young and immature leaves from emergency stage (VC), cotyledon stage (VE), vegetative stages 1, 2, 3, 4 and 5 (V1 to V5), and reproductive stages 1 and 2 (R1 and R2) were collected and extracted in 1:1 (v/v) methanol: water, and the extracts were analyzed by LC- MS. The main flavonoids of soybean leaves were identified by mass spectra and relatively quantified. During the developmental stages, higher levels of kaempferol mono, di and isomeric triglycerides found in immature and young leaves were found. The initial stages, emergency vegetative and coethylene vegetative, showed higher concentrations of isoflavones such as daidzein, genistein, glycitein and their glycoconjugates, which are more abundant in old leaves. The chemical profile and allocation dynamics of the studied flavonoids provide information on the different strategies used by the plant to allocate its resources, demonstrating that leaves of different ages differ in their composition, possibly according to their physiological and ecological context. Therefore, evidence of the role of constitutive flavonoids in the defense mechanism of soy will help in the development of methods that can contribute to agricultural productivity, adaptability and resistance to various stressors in a more sustainable way. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-05-26T11:47:57Z |
dc.date.available.fl_str_mv |
2021-05-26T11:47:57Z |
dc.date.issued.fl_str_mv |
2021-03-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Josicleia Oliveira. Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.].. 2021. Dissertação (Mestrado em Química) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/14309. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/14309 |
identifier_str_mv |
COSTA, Josicleia Oliveira. Perfil químico e dinâmica espacial e temporal de flavonoides de soja [Glycine Max (L.) Merr.].. 2021. Dissertação (Mestrado em Química) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/14309. |
url |
https://repositorio.ufscar.br/handle/ufscar/14309 |
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por |
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600 600 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química - PPGQ |
dc.publisher.initials.fl_str_mv |
UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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