Desenvolvimento de biomaterial incorporado com peptídeos antimicrobianos para aplicações em cicatrização de feridas e controle de infecções

• Ano de defesa: 2025
• Autor(a) principal: Santos, Pedro Augusto da Silva
• Orientador(a): Dias, Simoni Campos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Católica de Brasília
• Programa de Pós-Graduação: Programa Stricto Sensu em Ciências Genômicas e Biotecnologia
• Departamento: Escola de Saúde e Medicina
• País: Brasil
• Palavras-chave em Português: Mastoparano‑MO; Quitosana; Polivinilpirrolidona (PVP); Biomaterial; Feridas cutâneas; Cicatrização
• Palavras-chave em Inglês: Polyvinylpyrrolidone (PVP); Biomaterial; Skin wounds; Cicatrization
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS
• Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/3732

Management of cutaneous wounds requires solutions that promote tissue regeneration, control exudate, and achieve a sustained reduction of microbial burden while preserving cell viability. In this context, developing biomaterials capable of integrating wound-healing stimulation with antimicrobial action can support tissue regeneration and repair in hard-to-manage or hard-to-heal skin wounds, making such materials essential tools for advancing new wound-care technologies. This work develops a biomaterial containing antimicrobial peptides and, in an integrated manner, evaluates its physicochemical, pro-regenerative, and antimicrobial properties for application to skin wounds. In the initial phase, the study determined the minimum inhibitory concentrations, as well as the possible combined effect and interactions, of Mastoparan MO (Mast-MO) and Synoeca MP against clinically relevant fungi and bacteria associated with persistent infections in chronic wounds. In the second phase, a biomaterial consisting of chitosan (CS) and polyvinylpyrrolidone (PVP) incorporating the Mast-MO peptide was developed and characterized. Finally, in the last phase, the study assessed the biomaterial’s biocompatibility and its potential pro-regenerative effects in a model of primary human skin fibroblasts. In addition, the antimicrobial activity of the hydrogel was evaluated against pathogens commonly associated with persistent cutaneous infections. The results supported selecting the Mast-MO peptide as the ideal candidate for biomaterial development, which presented high biocompatibility, pro-regenerative properties in vitro and marked antibacterial activity, configuring this biomaterial as a promising platform for the development of biotechnological products that facilitate the management of cutaneous wounds.