Avaliação do efeito do uso de substratos exauridos de cultivo comercial de Ganoderma lucidum para o controle sustentável do fitonematoide Meloidogyne incognita

• Ano de defesa: 2022
• Autor(a) principal: Santos, João Nicanildo Bastos dos
• Orientador(a): Barreto, Cristine Chaves
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Católica de Brasília
• Programa de Pós-Graduação: Programa Stricto Sensu em Ciências Genômicas e Biotecnologia
• Departamento: Escola de Saúde e Medicina
• País: Brasil
• Palavras-chave em Português: Ganoderma; Substrato exaurido; Bioativos; Controle; Fitonematoide
• Palavras-chave em Inglês: Exhausted substrate; Bioactives; Control; Phytotnematode
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS
• Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/3074

Among phytonematodes, the genus Meloidogyne represents one of the most serious problems for world agriculture. The Meloidogyne incognita species stands out for causing extensive reduction in productivity and quality of agricultural products, generating economic losses of millions of dollars annually. The control of this phytoparasite occurs mainly by synthetic nematicides, which are problematic for human and animal health and also for the environment. Therefore, the search for control alternatives with sustainable technologies is favorable. Macrofungi substrates can represent a very rich source of biological actives with a biocidal effect. Thus, the objective of the present work was to evaluate the effect of extract generated from the macrofungus (Ganoderma lucidum mushroom) on the control of the root-knot nematode, Meloidogyne incognita. Through the cultivation of the fungus by the JunCao technique, an aqueous extract was generated and its effects were evaluated in viability and recovery bioassays with nematodes, confirming the extract's nematostatic and nematicidal activity. This activity was maintained when the material was subjected to a higher temperature, confirming the material's thermostability with viability and recovery bioassays. The material was further evaluated for its cellular toxicity, phytotoxicity and activity against non-target organisms. These results demonstrate the promising ability of using fungi and their metabolites in sustainable pest control.