Análise da microbiota em águas no Pantanal Sul: desenvolvimento de uma linha de base para monitoramento ambiental considerando variáveis hídricas, antrópicas e físico-químicas

• Ano de defesa: 2025
• Autor(a) principal: Parelho, Ana Regina Parelho
• Orientador(a): Pogue, Robert
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Católica de Brasília
• Programa de Pós-Graduação: Programa Stricto Sensu em Ciências Genômicas e Biotecnologia
• Departamento: Escola de Saúde e Medicina
• País: Brasil
• Palavras-chave em Português: Metagenoma; Pantanal; Sequenciamento oxford nanopore; Gene 16s rRNA; Microbiota; Monitoramento
• Palavras-chave em Inglês: Metagenome; Pantanal; Oxford nanopore sequencing; 16s rRNA gene; Microbiota; Monitoring
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS
• Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/3661

The Pantanal is the largest wetland ecosystem on the planet and harbors wealthy biodiversity, including various microorganisms. Bacteria play fundamental roles in the biogeochemical cycles of the Pantanal and aquatic ecosystems in general. They are involved in various vital processes, such as the decomposition of organic matter, nitrogen fixation, the carbon cycle, and the cycling of nutrients, including phosphorus, sulfur, and other essential elements. The objective of this dissertation is to establish a baseline for the future monitoring of aquatic microbiota in the flowing and stagnant waters of the Southern Pantanal. To this end, 12 samples were collected during two distinct seasons in May 2023 and February 2024 from water bodies in the Southern Pantanal. All DNA from the samples was extracted for 16S rRNA gene sequencing to identify the diversity profile and proportion of bacterial populations. This profile was analyzed across different locations and periods. The results indicate a bacterial profile characteristic of the sampled area, with an abundance of the genera Polynucleobacter and Limnohabitans in samples collected in 2023, while the samples from 2024 showed greater diversity. This baseline will enable the identification of alterations indicative of stress caused by anthropogenic activities, providing a foundation for decision-making aimed at conservation, biodiversity preservation, and environmental management in the Pantanal region.