Suplementação com levedura saccharomyces cerevisae reduz contaminação microbiológica da carcaça em novilhos terminados em confinamento

• Ano de defesa: 2020
• Autor(a) principal: Delazeri, Dailis
• Orientador(a): Bertagnon, Heloisa Godoi
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Centro-Oeste
• Programa de Pós-Graduação: Programa de Pós-Graduação em Medicina Veterinária (Mestrado)
• Departamento: Unicentro::Departamento de Medicina Veterinária
• País: Brasil
• Palavras-chave em Português: levedura; microrganismos; frigorífico
• Palavras-chave em Inglês: yeast; microorganisms; fridge
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://tede.unicentro.br:8080/jspui/handle/jspui/1790

The use of yeast cell wall feed additives (PCL) has already been studied with the aim of favouring the growth of beneficial intestinal bacteria to the detriment of pathogens in ruminants, thus decreasing diarrhoea and increasing animal performance. It is also believed that this change in the intestinal microbiota could contribute to less contamination of the bovine carcass at the time of slaughter, a fact that has not yet been studied in beef cattle. Thus, the present work aims at verifying if the prebiotic cell wall base of Saccharomyces cerevisiae reduces total coliforms, Escherichia coli and Salmonella spp from the feces and the bovine carcass at the time of slaughter after evisceration. For this purpose, 18 steers, ½ Angus blood, confined, were submitted to three daily treatments for 105 days: control (yeast-free diet), 4g yeast (4g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil), 7g yeast (7g/animal/day diet of 2 x 1010cel/g of Saccharomyces cerevisiae commercial RumenYeast®, ICC, São Paulo, Brazil). On the 29th and 90th days after entering the confinement, analyzes were made from samples of feces stool, leather, water and food samples were collected for Escherichia coli and total coliforms counting and isolation of Salmonella spp. based on the methodology described by the manufacturer Petrifilm 3M®. On the day of slaughter, the carcasses were collected for identification and quantification of Escherichia coli, total coliforms, mesophils by the same methodology and qualitative identification of Salmonella spp. by ISO 6579/2017 methodology. Reduction of Escherichia coli and total coliforms in stool samples (P=0.0001 and 0.001 respectively) and Escherichia coli, total coliforms and mesophilic aerobes in the carcass (P=0.06; 0.10 and 0.05 respectively) were noted in the groups treated with the two doses of the prebiotic, and that there was absence of Salmonella spp. in all collections performed. It was concluded that supplementation with 4 or 7 g/day of PCL reduced the fecal excretion and consequently the contamination of the carcass by Escherichia coli, mesophils and total coliforms of the confined animals, being a benign and residue-free sanitary measure to improve the microbiological quality of the meat.