NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: WILK, REBECA lattes
Orientador(a): Romano, Marco Aurélio lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual do Centro-Oeste
Programa de Pós-Graduação: Programa de Pós-Graduação em Medicina Veterinária (Mestrado)
Departamento: Unicentro::Departamento de Medicina Veterinária
País: Brasil
Palavras-chave em Português:
toxicidade, puberdade
desenvolvimento reprodutivo
desregulador endócrino
Palavras-chave em Inglês:
toxicity
reproductive development
endocrine disrupter
puberty
Área do conhecimento CNPq:
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
Link de acesso: http://tede.unicentro.br:8080/jspui/handle/jspui/784
Resumo: Silver nanoparticles AgNPs have been widely used as components of various commercial products both medical and industrial because of their bactericidal properties. Despite its wide range of use, its toxicological effects are still not well understood. They may present cytotoxicity, genotoxicity, blockage in mitochondrial activity, influence on development and weight, germ cell toxicity, and delay the age at puberty. The prepubertal period is a very sensitive period, where the hypothalamic pituitary testicular axis (HPT) undergoes morphophysiological changes, which may alter the hormonal levels and the onset of puberty. AgNPs are able to function as potential endocrine disrupting chemical, affecting spermatogenesis, but few studies report the effects on the hypothalamic-pituitary-gonadal axis. The objective was to verify the AgNP toxicity in rats through evaluation of body development, age at puberty, weight of the reproductive organs, and expression of genes related to HPT function and hormonal profile. Sixty rats were used in 5 groups treated with the doses: 0, 15, 7.5, 3.75 or 1.875 μg/kg. Puberty delay, increase in Gnrh mRNA expression, decreased Esr1 and Esr2 mRNA were observed. In addition, a decrease in mRNA in both Ar and Esr2 was observed, with an increase in Inhbb mRNA and in serum LH and FSH levels in the 3.75 and 1.875 groups respectively. It was concluded that the prebubertal AgNP exposure alters the gene expression in the HPT axis and alters the age at puberty and the hormonal serum levels.
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spelling Romano, Marco Auréliohttp://lattes.cnpq.br/1024434825015654076.678.389-84http://lattes.cnpq.br/6559761898537902WILK, REBECA2017-08-04T17:19:14Z2017-02-10WILK, REBECA. NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL. 2017. 49 f. Dissertação (Programa de Pós-Graduação em Medicina Veterinária - Mestrado) - Universidade Estadual do Centro-Oeste, Guarapuava - PR.http://tede.unicentro.br:8080/jspui/handle/jspui/784Silver nanoparticles AgNPs have been widely used as components of various commercial products both medical and industrial because of their bactericidal properties. Despite its wide range of use, its toxicological effects are still not well understood. They may present cytotoxicity, genotoxicity, blockage in mitochondrial activity, influence on development and weight, germ cell toxicity, and delay the age at puberty. The prepubertal period is a very sensitive period, where the hypothalamic pituitary testicular axis (HPT) undergoes morphophysiological changes, which may alter the hormonal levels and the onset of puberty. AgNPs are able to function as potential endocrine disrupting chemical, affecting spermatogenesis, but few studies report the effects on the hypothalamic-pituitary-gonadal axis. The objective was to verify the AgNP toxicity in rats through evaluation of body development, age at puberty, weight of the reproductive organs, and expression of genes related to HPT function and hormonal profile. Sixty rats were used in 5 groups treated with the doses: 0, 15, 7.5, 3.75 or 1.875 μg/kg. Puberty delay, increase in Gnrh mRNA expression, decreased Esr1 and Esr2 mRNA were observed. In addition, a decrease in mRNA in both Ar and Esr2 was observed, with an increase in Inhbb mRNA and in serum LH and FSH levels in the 3.75 and 1.875 groups respectively. It was concluded that the prebubertal AgNP exposure alters the gene expression in the HPT axis and alters the age at puberty and the hormonal serum levels.Nanopartículas de prata AgNPs, tem sido amplamente utilizadas como componentes de diversos produtos comerciais tanto médicos quanto industriais, em função de suas propriedades bactericidas. Apesar da sua ampla gama de utilização, ainda não se conhece muito bem seus efeitos toxicológicos. Elas podem apresentar citotoxicidade, genotoxicidade, bloqueio na atividade mitocondrial, influenciam no desenvolvimento e peso, toxicidade sobre células germinativas e atraso na idade à puberdade. A fase pré-púbere é o período muito sensível, onde o eixo hipotalâmico hipofisário testicular (HHT) sofre alterações morfofisiológicas, que podem alterar os níveis hormonais e início da puberdade animal. As AgNPs são capazes de agir como potencial desreguladores endócrinos, afetando marcadamente a espermatogênese, porém são poucos trabalhos relatam seus efeitos sobre o eixo hipotalâmico-hipofisário-gonadal. O objetivo deste estudo foi verificar a toxicidade das AgNPs em ratos, mediante avaliação do desenvolvimento corporal, idade à puberdade, peso dos órgãos reprodutivos, além da expressão de genes relacionados à função HHT e perfil hormonal. Foram utilizados 60 ratos em 5 grupos tratados com as doses: 0, 15, 7,5, 3,75 ou 1,875 μg/kg. Constatou-se atraso na puberdade, aumento da expressão do mRNA do Gnrh diminuição do mRNA do Esr1 e do Esr2 no hipotálamo. Além disso, observou-se uma diminuição do mRNA nos Ar e do Esr2 na hipófise, e ocorreu um aumento do mRNA da Inhbb e nos níveis séricos da LH e FSH nos grupos de 3,75 e 1,875 respectivamente. Concluiu-se que a exposição pré-pubere as AgNP altera a expressão gênica no eixo HHT, bem como da idade à puberdade e os níveis séricos de LH e FSH.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2017-08-04T17:19:14Z No. of bitstreams: 1 REBECA WILK.pdf: 822626 bytes, checksum: 5aa3184bc19366409fc794ac04057c44 (MD5)Made available in DSpace on 2017-08-04T17:19:14Z (GMT). No. of bitstreams: 1 REBECA WILK.pdf: 822626 bytes, checksum: 5aa3184bc19366409fc794ac04057c44 (MD5) Previous issue date: 2017-01-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.unicentro.br:8080/jspui/retrieve/3694/REBECA%20WILK.pdf.jpgporUniversidade Estadual do Centro-OestePrograma de Pós-Graduação em Medicina Veterinária (Mestrado)UNICENTROBrasilUnicentro::Departamento de Medicina Veterináriatoxicidade, puberdadedesenvolvimento reprodutivodesregulador endócrinotoxicityreproductive developmentendocrine disrupterpubertyCIENCIAS AGRARIAS::MEDICINA VETERINARIANANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADALSilver nanoparticles (AGNPS) alter the hypothalamic pituitary and gonadal gene expressioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis635658161267837813360060060060074005008467382129864536702642350173192075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROTHUMBNAILREBECA WILK.pdf.jpgREBECA WILK.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/784/4/REBECA+WILK.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD54TEXTREBECA WILK.pdf.txtREBECA WILK.pdf.txttext/plain94965http://localhost:8080/tede/bitstream/jspui/784/3/REBECA+WILK.pdf.txtfb219897f2448f162c7644ec6214b910MD53ORIGINALREBECA WILK.pdfREBECA WILK.pdfapplication/pdf822626http://localhost:8080/tede/bitstream/jspui/784/2/REBECA+WILK.pdf5aa3184bc19366409fc794ac04057c44MD52LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
dc.title.alternative.eng.fl_str_mv Silver nanoparticles (AGNPS) alter the hypothalamic pituitary and gonadal gene expression
title NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
spellingShingle NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
WILK, REBECA
toxicidade, puberdade
desenvolvimento reprodutivo
desregulador endócrino
toxicity
reproductive development
endocrine disrupter
puberty
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
title_full NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
title_fullStr NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
title_full_unstemmed NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
title_sort NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL
author WILK, REBECA
author_facet WILK, REBECA
author_role author
dc.contributor.advisor1.fl_str_mv Romano, Marco Aurélio
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1024434825015654
dc.contributor.authorID.fl_str_mv 076.678.389-84
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6559761898537902
dc.contributor.author.fl_str_mv WILK, REBECA
contributor_str_mv Romano, Marco Aurélio
dc.subject.por.fl_str_mv toxicidade, puberdade
desenvolvimento reprodutivo
desregulador endócrino
topic toxicidade, puberdade
desenvolvimento reprodutivo
desregulador endócrino
toxicity
reproductive development
endocrine disrupter
puberty
CIENCIAS AGRARIAS::MEDICINA VETERINARIA
dc.subject.eng.fl_str_mv toxicity
reproductive development
endocrine disrupter
puberty
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description Silver nanoparticles AgNPs have been widely used as components of various commercial products both medical and industrial because of their bactericidal properties. Despite its wide range of use, its toxicological effects are still not well understood. They may present cytotoxicity, genotoxicity, blockage in mitochondrial activity, influence on development and weight, germ cell toxicity, and delay the age at puberty. The prepubertal period is a very sensitive period, where the hypothalamic pituitary testicular axis (HPT) undergoes morphophysiological changes, which may alter the hormonal levels and the onset of puberty. AgNPs are able to function as potential endocrine disrupting chemical, affecting spermatogenesis, but few studies report the effects on the hypothalamic-pituitary-gonadal axis. The objective was to verify the AgNP toxicity in rats through evaluation of body development, age at puberty, weight of the reproductive organs, and expression of genes related to HPT function and hormonal profile. Sixty rats were used in 5 groups treated with the doses: 0, 15, 7.5, 3.75 or 1.875 μg/kg. Puberty delay, increase in Gnrh mRNA expression, decreased Esr1 and Esr2 mRNA were observed. In addition, a decrease in mRNA in both Ar and Esr2 was observed, with an increase in Inhbb mRNA and in serum LH and FSH levels in the 3.75 and 1.875 groups respectively. It was concluded that the prebubertal AgNP exposure alters the gene expression in the HPT axis and alters the age at puberty and the hormonal serum levels.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-08-04T17:19:14Z
dc.date.issued.fl_str_mv 2017-02-10
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dc.identifier.citation.fl_str_mv WILK, REBECA. NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL. 2017. 49 f. Dissertação (Programa de Pós-Graduação em Medicina Veterinária - Mestrado) - Universidade Estadual do Centro-Oeste, Guarapuava - PR.
dc.identifier.uri.fl_str_mv http://tede.unicentro.br:8080/jspui/handle/jspui/784
identifier_str_mv WILK, REBECA. NANOPARTÍCULAS DE PRATA (AGNPS) ALTERAM A EXPRESSÃO GÊNICA HIPOTALÂMICA HIPOFISÁRIA E GONADAL. 2017. 49 f. Dissertação (Programa de Pós-Graduação em Medicina Veterinária - Mestrado) - Universidade Estadual do Centro-Oeste, Guarapuava - PR.
url http://tede.unicentro.br:8080/jspui/handle/jspui/784
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dc.publisher.department.fl_str_mv Unicentro::Departamento de Medicina Veterinária
publisher.none.fl_str_mv Universidade Estadual do Centro-Oeste
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