Efeito do óleo essencial de Pterodon polygalaeflorus Benth em músculo liso intestinal de rato

• Ano de defesa: 2008
• Autor(a) principal: Leonhardt, Valéria
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Ceará
• Palavras-chave em Português: Fisiologia; Músculo liso; Oleo Essencial - Pterodon Polygalaeflorus; Ratos
• Link de acesso: https://siduece.uece.br/siduece/trabalhoAcademicoPublico.jsf?id=65670

<span style="left: 26.22%; top: 14.31%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">O</span><span style="left: 27.67%; top: 14.31%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 28.5%; top: 14.31%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.941041);" role="presentation" dir="ltr">Pterodon polygalaeflorus</span><span style="left: 49.49%; top: 14.31%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 50.32%; top: 14.31%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.938926);" role="presentation" dir="ltr">Benth, popularmente conhecido como “sucupira </span><span style="left: 14.3%; top: 16.76%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.984021);" role="presentation" dir="ltr">branca”, é largamente utilizada na medicina popular, entre outros para o tratamento de </span><span style="left: 14.3%; top: 19.23%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.927668);" role="presentation" dir="ltr">diarréias crônicas no Nordeste do Brasil. O óleo essencial extraído das sementes dos frutos </span><span style="left: 14.3%; top: 21.68%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.937504);" role="presentation" dir="ltr">maduros apresentou como principal constituinte o &#946;-cariofileno ou também chamado trans-</span><span style="left: 14.3%; top: 24.14%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.918194);" role="presentation" dir="ltr">cariofileno (~ 27%). O presente trabalho teve como objetivo avaliar o efeito do óleo essencial</span><br role="presentation"/><span style="left: 14.3%; top: 26.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.848421);" role="presentation" dir="ltr">de</span><span style="left: 16.2%; top: 26.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 16.74%; top: 26.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.929289);" role="presentation" dir="ltr">Pterodon polygalaeflorus</span><span style="left: 37.47%; top: 26.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 38.02%; top: 26.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.917868);" role="presentation" dir="ltr">(OEPpgf), e seu constituinte &#946;-cariofileno, sobre a contratilidade </span><span style="left: 14.3%; top: 29.06%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.901955);" role="presentation" dir="ltr">de intestino de rato</span><span style="left: 29.77%; top: 29.06%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 30.29%; top: 29.06%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998462);" role="presentation" dir="ltr">in vitro</span><span style="left: 36.22%; top: 29.06%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.905566);" role="presentation" dir="ltr">. Segmentos de íleo (1 a 2 cm), mantidos em solução fisiológica de </span><span style="left: 14.3%; top: 31.51%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.921168);" role="presentation" dir="ltr">Tyrode, aerados, a 37°C e pH 7,4, foram utilizados para registro das contrações isométricas. </span><span style="left: 14.3%; top: 33.98%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.939487);" role="presentation" dir="ltr">O OEPpgf (3 a 1300&#956;g/ml) e seu constituinte (0,1 a 1000&#956;g/ml) foram capazes de alterar o </span><span style="left: 14.3%; top: 36.43%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.992964);" role="presentation" dir="ltr">tônus basal do músculo liso intestinal, com CI</span><span style="left: 54.78%; top: 36.97%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.870796);" role="presentation" dir="ltr">50</span><span style="left: 56.14%; top: 36.97%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 57.12%; top: 36.43%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.963642);" role="presentation" dir="ltr">de 578,42±177,98 e 68,65±0,94 &#956;g/ml, </span><span style="left: 14.3%; top: 38.9%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.94593);" role="presentation" dir="ltr">respectivamente. Na presença de hexametônio, indometacina e L-NAME as alterações no </span><span style="left: 14.3%; top: 41.35%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.968048);" role="presentation" dir="ltr">tônus não foram diferentes das descritas anteriormente. O OEPpgf e &#946;-cariofileno foram </span><span style="left: 14.3%; top: 43.81%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.93086);" role="presentation" dir="ltr">capazes de relaxar contrações mantidas induzidas por 60 mM de K</span><span style="left: 70.33%; top: 43.51%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">+</span><span style="left: 71.09%; top: 43.81%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.989535);" role="presentation" dir="ltr">, com CI</span><span style="left: 78.47%; top: 44.35%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.870796);" role="presentation" dir="ltr">50</span><span style="left: 79.83%; top: 44.35%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 80.57%; top: 43.81%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.938134);" role="presentation" dir="ltr">de 144,43 ± </span><span style="left: 14.3%; top: 46.26%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.908739);" role="presentation" dir="ltr">39,28 e 34,60 ± 0,94 &#956;g/ml, respectivamente. O OEPpgf e seu constituinte, adicionados 5 min </span><span style="left: 14.3%; top: 48.73%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.960314);" role="presentation" dir="ltr">antes da adição de agente contraturante, inibiu, de maneira concentração dependente, a </span><span style="left: 14.3%; top: 51.18%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.942805);" role="presentation" dir="ltr">contração induzida por k</span><span style="left: 35.01%; top: 50.88%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">+</span><span style="left: 35.78%; top: 51.18%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00028);" role="presentation" dir="ltr">, com CI</span><span style="left: 43.24%; top: 51.72%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.870796);" role="presentation" dir="ltr">50</span><span style="left: 44.6%; top: 51.72%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 45.36%; top: 51.18%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.945308);" role="presentation" dir="ltr">189,41 ± 31,40 e 8,30 ± 5,32 &#956;g/ml, respactivamente. </span><span style="left: 14.3%; top: 53.63%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.925507);" role="presentation" dir="ltr">Quando a ACh foi utilizada como agente contraturante, o OEPpgf e seu constituinte inibiram </span><span style="left: 14.3%; top: 56.1%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.962459);" role="presentation" dir="ltr">a contração com CI</span><span style="left: 31.08%; top: 56.64%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.870796);" role="presentation" dir="ltr">50</span><span style="left: 32.44%; top: 56.64%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 33.32%; top: 56.1%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.968865);" role="presentation" dir="ltr">de 58,45 ± 2,53 e 13,53 ± 11,17 &#956;g/ml,  respectivamente. Sendo, o </span><span style="left: 14.3%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.915407);" role="presentation" dir="ltr">OEPpgf,</span><span style="left: 21.28%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 22.55%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.895012);" role="presentation" dir="ltr">mais</span><span style="left: 26.36%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 27.61%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.90212);" role="presentation" dir="ltr">específico</span><span style="left: 35.8%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 37.05%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.876529);" role="presentation" dir="ltr">em</span><span style="left: 39.51%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 40.76%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.900154);" role="presentation" dir="ltr">bloquear</span><span style="left: 47.83%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 49.09%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.785082);" role="presentation" dir="ltr">as</span><span style="left: 50.76%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 52.02%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.872949);" role="presentation" dir="ltr">contrações</span><span style="left: 60.63%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 61.9%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.908323);" role="presentation" dir="ltr">induzidas</span><span style="left: 69.64%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 70.89%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.880477);" role="presentation" dir="ltr">pela</span><span style="left: 74.25%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 75.51%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.971292);" role="presentation" dir="ltr">ACh</span><span style="left: 79.32%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 80.57%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.919037);" role="presentation" dir="ltr">(t-test).</span><span style="left: 86.44%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 87.69%; top: 58.55%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.931919);" role="presentation" dir="ltr">Em </span><span style="left: 14.3%; top: 61.01%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.95421);" role="presentation" dir="ltr">preparações mantidas em solução despolarizante (K</span><span style="left: 59.21%; top: 60.72%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">+</span><span style="left: 59.98%; top: 60.72%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 61.11%; top: 61.01%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03817);" role="presentation" dir="ltr">60 mM) sem Ca</span><span style="left: 75.99%; top: 60.72%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 77.44%; top: 60.72%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 78.57%; top: 61.01%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05192);" role="presentation" dir="ltr">e com EGTA </span><span style="left: 14.3%; top: 63.47%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.972019);" role="presentation" dir="ltr">(0,2mM), o OEPpgf (300&#956;g/ml) e seu constituinte (100&#956;g/ml) reduziram a amplitude das</span><br role="presentation"/><span style="left: 14.3%; top: 65.93%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.957863);" role="presentation" dir="ltr">contrações induzidas pela adição crescente e cumulativa de Ca</span><span style="left: 68.83%; top: 65.63%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 70.28%; top: 65.63%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 71.29%; top: 65.93%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997784);" role="presentation" dir="ltr">para 10,49% ± 2,27 e </span><span style="left: 14.3%; top: 68.38%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.92921);" role="presentation" dir="ltr">16,66% ± 5,30 da contração controle, respectivamente. O OEPpgf (300&#956;g/mL, n=6) e trans-</span><span style="left: 14.3%; top: 70.85%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.916681);" role="presentation" dir="ltr">cariofileno (100&#956;g/mL, n=6) foram capazes de bloquear as contrações induzidas por ACh em </span><span style="left: 14.3%; top: 73.3%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.920908);" role="presentation" dir="ltr">meio sem Ca</span><span style="left: 25.23%; top: 73%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 26.68%; top: 73.3%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.924822);" role="presentation" dir="ltr">, com significante redução de 48,70±3,50 e 40,71±1,85%, respectivamente. O </span><span style="left: 14.3%; top: 75.76%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.897554);" role="presentation" dir="ltr">OEPpgf e seu constituinte foram capazes de reduzir o pico das contrações induzidas por Ba</span><span style="left: 88.24%; top: 75.47%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 89.68%; top: 75.47%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 90.05%; top: 75.76%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">, </span><span style="left: 14.3%; top: 78.22%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.918466);" role="presentation" dir="ltr">em preparações mantidas em solução despolarizante (K</span><span style="left: 60.63%; top: 77.92%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">+</span><span style="left: 61.39%; top: 77.92%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 62.16%; top: 78.22%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.955971);" role="presentation" dir="ltr">60mM) em meio sem Ca</span><span style="left: 83.25%; top: 77.92%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.913194);" role="presentation" dir="ltr">2+</span><span style="left: 84.69%; top: 78.22%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.918391);" role="presentation" dir="ltr">, assim </span><span style="left: 14.3%; top: 80.68%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.909182);" role="presentation" dir="ltr">como o verapamil um bloqueador de VOC, para 56,76% ± 4,03 e 37,84% ± 3,55 da contração </span><span style="left: 14.3%; top: 83.13%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.924671);" role="presentation" dir="ltr">controle, respectivamente. Após contrações sucessivas induzidas por carbacol (100&#956;M), em </span><span style="left: 14.3%; top: 85.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.914117);" role="presentation" dir="ltr">meio sem Ca</span><span style="left: 25.15%; top: 85.3%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 26.6%; top: 85.3%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 27.27%; top: 85.6%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.924725);" role="presentation" dir="ltr">e contendo verapamil(1&#956;M), o OEPpgf e seu constituinte não foram capazes </span><span style="left: 14.3%; top: 88.05%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.936457);" role="presentation" dir="ltr">de bloquear as contrações induzidas por adição de Ca</span><span style="left: 59.96%; top: 87.75%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.916137);" role="presentation" dir="ltr">2+.</span><span style="left: 61.75%; top: 87.75%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 62.56%; top: 88.05%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.95251);" role="presentation" dir="ltr">O OEPpgf e seu constituinte não </span><span style="left: 14.3%; top: 90.52%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.935571);" role="presentation" dir="ltr">foram capazes de bloquear as contrações induzidas por ACh em meio sem Ca</span><span style="left: 80.15%; top: 90.22%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 81.6%; top: 90.22%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 82.37%; top: 90.52%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.932156);" role="presentation" dir="ltr">em tecido </span><span style="left: 14.3%; top: 10.32%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.92406);" role="presentation" dir="ltr">com estoques de Ca</span><span style="left: 31.38%; top: 10.02%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif; transform: scaleX(0.926098);" role="presentation" dir="ltr">2+</span><span style="left: 32.83%; top: 10.02%; font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: 33.68%; top: 10.32%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.945607);" role="presentation" dir="ltr">depletados por 60&#956;M de ACh. Em conclusão, os nossos resultados </span><span style="left: 14.3%; top: 12.77%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.943077);" role="presentation" dir="ltr">sugerem que o efeito miorelaxante induzido pelo OEPpgf e seu constituinte é mediado por </span><span style="left: 14.3%; top: 15.24%; font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.890922);" role="presentation" dir="ltr">mecanismos intracelulares, sem poder descartar o efeito sobre o acoplamento eletr</span>omecânico.