Expressão e produção de peptídeo imunogênico do envelope do vírus dengue em sistema vegetal

• Ano de defesa: 2011
• Autor(a) principal: Silva, Isaac Neto Goes da
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Ceará
• Palavras-chave em Português: Biotecnologia; Dengue - Vacina; Sistema vegetal
• Link de acesso: https://siduece.uece.br/siduece/trabalhoAcademicoPublico.jsf?id=71031

<span class="markedContent" id="page17R_mcid2"><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">A</span><span style="left: calc(var(--scale-factor)*93.08px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*100.32px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0008);" role="presentation" dir="ltr">Dengue</span><span style="left: calc(var(--scale-factor)*142.39px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*149.64px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">é</span><span style="left: calc(var(--scale-factor)*156.31px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*163.56px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.99608);" role="presentation" dir="ltr">causada</span><span style="left: calc(var(--scale-factor)*208.75px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*216.00px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.10298);" role="presentation" dir="ltr">por um</span><span style="left: calc(var(--scale-factor)*257.20px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*264.48px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.992372);" role="presentation" dir="ltr">vírus</span><span style="left: calc(var(--scale-factor)*290.28px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*297.60px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998316);" role="presentation" dir="ltr">que</span><span style="left: calc(var(--scale-factor)*317.59px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*324.84px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00177);" role="presentation" dir="ltr">apresenta</span><span style="left: calc(var(--scale-factor)*378.31px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*385.56px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.996521);" role="presentation" dir="ltr">quatro</span><span style="left: calc(var(--scale-factor)*419.47px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*426.72px); top: calc(var(--scale-factor)*128.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03706);" role="presentation" dir="ltr">diferentes sorotipos, </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00102);" role="presentation" dir="ltr">antigenicamente</span><span style="left: calc(var(--scale-factor)*173.23px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*180.96px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00066);" role="presentation" dir="ltr">distintos,</span><span style="left: calc(var(--scale-factor)*228.34px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*236.04px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00142);" role="presentation" dir="ltr">pertencentes</span><span style="left: calc(var(--scale-factor)*305.52px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*313.20px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.994122);" role="presentation" dir="ltr">ao</span><span style="left: calc(var(--scale-factor)*326.47px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*334.32px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00048);" role="presentation" dir="ltr">gênero</span><span style="left: calc(var(--scale-factor)*371.71px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*379.44px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999527);" role="presentation" dir="ltr">Flavivirus</span><span style="left: calc(var(--scale-factor)*430.08px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">.</span><span style="left: calc(var(--scale-factor)*433.42px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*441.12px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997916);" role="presentation" dir="ltr">Devido</span><span style="left: calc(var(--scale-factor)*478.39px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*486.24px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00479);" role="presentation" dir="ltr">aos</span><span style="left: calc(var(--scale-factor)*505.68px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*513.48px); top: calc(var(--scale-factor)*149.06px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999049);" role="presentation" dir="ltr">seus </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*169.94px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.04483);" role="presentation" dir="ltr">fatores de virulência, entre eles, notadamente a proteína E, estes vírus tornam-se </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*190.58px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02599);" role="presentation" dir="ltr">responsáveis por mais de 50 milhões de infecções a cada ano, com 500.000 casos</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*211.34px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05106);" role="presentation" dir="ltr">de dengue hemorrágica e pelo menos 25.000 mortes. Até o momento, a principal</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*231.98px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.099);" role="presentation" dir="ltr">forma de combate, ainda é o controle dos mosquitos transmissores, e poucos</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00153);" role="presentation" dir="ltr">avanços</span><span style="left: calc(var(--scale-factor)*129.84px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*138.00px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999084);" role="presentation" dir="ltr">ocorreram</span><span style="left: calc(var(--scale-factor)*192.64px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*200.76px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.994122);" role="presentation" dir="ltr">no</span><span style="left: calc(var(--scale-factor)*214.03px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*222.24px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998316);" role="presentation" dir="ltr">que</span><span style="left: calc(var(--scale-factor)*242.23px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*250.44px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.990526);" role="presentation" dir="ltr">se</span><span style="left: calc(var(--scale-factor)*262.99px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*271.20px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997415);" role="presentation" dir="ltr">refere</span><span style="left: calc(var(--scale-factor)*302.47px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*310.68px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">à</span><span style="left: calc(var(--scale-factor)*317.35px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*325.32px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00074);" role="presentation" dir="ltr">vacinação</span><span style="left: calc(var(--scale-factor)*379.39px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*387.60px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998801);" role="presentation" dir="ltr">contra</span><span style="left: calc(var(--scale-factor)*420.91px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*429.00px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">a</span><span style="left: calc(var(--scale-factor)*435.67px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*443.76px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00353);" role="presentation" dir="ltr">dengue.</span><span style="left: calc(var(--scale-factor)*487.30px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*495.36px); top: calc(var(--scale-factor)*252.74px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00187);" role="presentation" dir="ltr">Estudos</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0001);" role="presentation" dir="ltr">envolvendo</span><span style="left: calc(var(--scale-factor)*146.47px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*153.96px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00384);" role="presentation" dir="ltr">outras</span><span style="left: calc(var(--scale-factor)*187.44px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*194.88px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997789);" role="presentation" dir="ltr">viroses</span><span style="left: calc(var(--scale-factor)*232.80px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*240.24px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997726);" role="presentation" dir="ltr">têm</span><span style="left: calc(var(--scale-factor)*260.20px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*267.72px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999777);" role="presentation" dir="ltr">demonstrado</span><span style="left: calc(var(--scale-factor)*337.75px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*345.24px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998316);" role="presentation" dir="ltr">que</span><span style="left: calc(var(--scale-factor)*365.23px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*372.72px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00005);" role="presentation" dir="ltr">dentre</span><span style="left: calc(var(--scale-factor)*406.75px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*414.24px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00378);" role="presentation" dir="ltr">as</span><span style="left: calc(var(--scale-factor)*426.96px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*434.40px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997275);" role="presentation" dir="ltr">várias</span><span style="left: calc(var(--scale-factor)*466.32px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*473.76px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00334);" role="presentation" dir="ltr">técnicas</span><span style="left: calc(var(--scale-factor)*517.92px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*525.36px); top: calc(var(--scale-factor)*273.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00311);" role="presentation" dir="ltr">de </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*294.14px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01224);" role="presentation" dir="ltr">ensaios vacinais desenvolvidas, as que utilizam vírus vegetais como carreadores de</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*314.78px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01824);" role="presentation" dir="ltr">peptídeos de interesse médico têm apresentado resultados promissores. Com base</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*335.54px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.08296);" role="presentation" dir="ltr">nesta informação e no atual impacto socioeconômico do dengue, este trabalho</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00077);" role="presentation" dir="ltr">objetivou</span><span style="left: calc(var(--scale-factor)*133.15px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*140.64px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997168);" role="presentation" dir="ltr">produzir</span><span style="left: calc(var(--scale-factor)*183.88px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*191.28px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01057);" role="presentation" dir="ltr">uma</span><span style="left: calc(var(--scale-factor)*214.87px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*222.36px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998724);" role="presentation" dir="ltr">estratégia</span><span style="left: calc(var(--scale-factor)*274.99px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*282.48px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999843);" role="presentation" dir="ltr">vacinal</span><span style="left: calc(var(--scale-factor)*319.82px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*327.24px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0024);" role="presentation" dir="ltr">contra</span><span style="left: calc(var(--scale-factor)*360.67px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*368.16px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">o</span><span style="left: calc(var(--scale-factor)*374.83px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*382.32px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00076);" role="presentation" dir="ltr">dengue,</span><span style="left: calc(var(--scale-factor)*425.74px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*433.20px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.997863);" role="presentation" dir="ltr">utilizando</span><span style="left: calc(var(--scale-factor)*483.79px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*491.28px); top: calc(var(--scale-factor)*356.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00396);" role="presentation" dir="ltr">sistemas </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*376.94px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03804);" role="presentation" dir="ltr">vegetais para a expressão de peptídeos derivados da proteína E do vírus dengue.</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*397.58px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03687);" role="presentation" dir="ltr">Para tanto, segmentos iniciadores contendo sítios de restrição foram desenhados, </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*418.34px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00769);" role="presentation" dir="ltr">clonados e inseridos em um plasmídeo não comercial referente ao Vírus do Mosaico</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*438.86px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.10967);" role="presentation" dir="ltr">do Caupi (CPMV). A quimera gerada foi inoculada em folhas de planta</span><span style="left: calc(var(--scale-factor)*501.07px); top: calc(var(--scale-factor)*438.86px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*508.08px); top: calc(var(--scale-factor)*438.86px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00627);" role="presentation" dir="ltr">Vigna </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*459.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00206);" role="presentation" dir="ltr">unguiculata</span><span style="left: calc(var(--scale-factor)*146.59px); top: calc(var(--scale-factor)*459.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*150.84px); top: calc(var(--scale-factor)*459.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02019);" role="presentation" dir="ltr">mantidas em casas de vegetação. Folhas infectadas que apresentaram </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*480.38px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00756);" role="presentation" dir="ltr">sintomas dias após a infecção foram colhidas e processadas para a obtenção de um</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*501.02px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.03269);" role="presentation" dir="ltr">extrato. Visando verificar a presença do peptídeo produzido na planta, o extrato foi</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*521.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.06965);" role="presentation" dir="ltr">submetido à eletroforese em gel de poliacrilamida e</span><span style="left: calc(var(--scale-factor)*379.03px); top: calc(var(--scale-factor)*521.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*385.08px); top: calc(var(--scale-factor)*521.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0359);" role="presentation" dir="ltr">Western blotting</span><span style="left: calc(var(--scale-factor)*474.60px); top: calc(var(--scale-factor)*521.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.09486);" role="presentation" dir="ltr">. Assim, foi</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00044);" role="presentation" dir="ltr">obtido</span><span style="left: calc(var(--scale-factor)*117.79px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*125.40px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00276);" role="presentation" dir="ltr">um</span><span style="left: calc(var(--scale-factor)*142.12px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*149.76px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998232);" role="presentation" dir="ltr">peptídeo</span><span style="left: calc(var(--scale-factor)*196.39px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*204.00px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00088);" role="presentation" dir="ltr">cuja</span><span style="left: calc(var(--scale-factor)*226.03px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*233.64px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0001);" role="presentation" dir="ltr">capacidade</span><span style="left: calc(var(--scale-factor)*295.03px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*302.64px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00093);" role="presentation" dir="ltr">imunogênica</span><span style="left: calc(var(--scale-factor)*370.75px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*378.24px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01515);" role="presentation" dir="ltr">foi</span><span style="left: calc(var(--scale-factor)*391.10px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*398.64px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998758);" role="presentation" dir="ltr">testada</span><span style="left: calc(var(--scale-factor)*437.95px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*445.44px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00276);" role="presentation" dir="ltr">em</span><span style="left: calc(var(--scale-factor)*462.16px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*469.80px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00126);" role="presentation" dir="ltr">animais</span><span style="left: calc(var(--scale-factor)*511.20px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*518.76px); top: calc(var(--scale-factor)*542.42px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.998316);" role="presentation" dir="ltr">que </span><span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*563.18px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05451);" role="presentation" dir="ltr">produziram anticorpos protetores. Tal resultado representa um avanço na corrida</span> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*583.82px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00039);" role="presentation" dir="ltr">para o desenvolvimento de uma vacina eficaz contra o vírus dengue.</span></span><span class="markedContent" id="page17R_mcid3"></span><span class="markedContent" id="page17R_mcid4"> <span style="left: calc(var(--scale-factor)*85.08px); top: calc(var(--scale-factor)*625.22px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.0618);" role="presentation" dir="ltr">Palavras-chave:</span><span style="left: calc(var(--scale-factor)*176.44px); top: calc(var(--scale-factor)*625.22px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*179.88px); top: calc(var(--scale-factor)*625.22px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00562);" role="presentation" dir="ltr">Dengue; Vacina; Vírus vegetal.</span></span>