Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
| Ano de defesa: | 2012 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
| Programa de Pós-Graduação: |
Mestrado Acad?mico em Recursos Gen?ticos Vegetais
|
| Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
| País: |
Brasil
|
| Palavras-chave em Português: | |
| Palavras-chave em Inglês: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | http://tede2.uefs.br:8080/handle/tede/951 |
Resumo: | Amburana cearensis AC Smith, popularly known as "imburana?de-cheiro", "amburana-de-cheiro" and "cumaru", has wide distribution in regions of Caatinga and Cerrado. It stands out due to its multitude of uses, such as forage, food, timber and medicinal. By virtue of degradation of their natural habitats and predatory exploitation, it is listed as endangered species. The aim this study was to optimize the micropropagation protocol and develop a protocol for in vitro conservation Amburana cearensis. In the first chapter, for in vitro multiplication, were used different plant regulators (BAP, ANA, AIA, KIN) and explants (zygotic embryo, nodal and cotyledonary segments; in stage of rooting were tested auxins AIA, AIB, ANA and AIB combination with activated carbon . In the second chapter, was evaluated the effect of osmotic agents sucrose, sorbitol and mannitol and growth retardants paclobutrazol and ancymidol on vitro conservation of A. cearensis. The medium used in the experiments was the WPM supplemented with 87.64 mM sucrose, except in treatments using osmotic agent that contained variations in the concentration of sucrose. The best result was achieved for multiplication using nodal segments in culture environment added with1?M BAP and 0.05 ?M ANA. The addition of 2 mM activated carbon associated with4?M AIB gave the best rooting percentages of shoots (54%). Supplementation of 262.92mM sucrose in the environment enabled the preservation of plants A. cearensis for 300 days with 77.5% survival. |
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Santana, Jos? Raniere Ferreira de55155448549http://lattes.cnpq.br/1799013075616667Alvim, Bruno Freitas Matos2020-01-09T22:24:11Z2012-05-28ALVIM, Bruno Freitas Matos. Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith. 2012. 84 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.http://tede2.uefs.br:8080/handle/tede/951Amburana cearensis AC Smith, popularly known as "imburana?de-cheiro", "amburana-de-cheiro" and "cumaru", has wide distribution in regions of Caatinga and Cerrado. It stands out due to its multitude of uses, such as forage, food, timber and medicinal. By virtue of degradation of their natural habitats and predatory exploitation, it is listed as endangered species. The aim this study was to optimize the micropropagation protocol and develop a protocol for in vitro conservation Amburana cearensis. In the first chapter, for in vitro multiplication, were used different plant regulators (BAP, ANA, AIA, KIN) and explants (zygotic embryo, nodal and cotyledonary segments; in stage of rooting were tested auxins AIA, AIB, ANA and AIB combination with activated carbon . In the second chapter, was evaluated the effect of osmotic agents sucrose, sorbitol and mannitol and growth retardants paclobutrazol and ancymidol on vitro conservation of A. cearensis. The medium used in the experiments was the WPM supplemented with 87.64 mM sucrose, except in treatments using osmotic agent that contained variations in the concentration of sucrose. The best result was achieved for multiplication using nodal segments in culture environment added with1?M BAP and 0.05 ?M ANA. The addition of 2 mM activated carbon associated with4?M AIB gave the best rooting percentages of shoots (54%). Supplementation of 262.92mM sucrose in the environment enabled the preservation of plants A. cearensis for 300 days with 77.5% survival.Amburana cearensis A.C. Smith, conhecida popularmente como imburana-de-cheiro, amburana-de-cheiro e cumaru, tem ampla distribui??o nas regi?es de Caatinga e Cerrado. Destaca-se devido a sua multiplicidade de usos, tais como forrageiro, alimentar, madeireiro e medicinal. Em virtude da degrada??o de seus h?bitats naturais e sua explora??o predat?ria, ? listada como esp?cie amea?ada de extin??o. O objetivo deste estudo foi otimizar o protocolo de micropropaga??o e desenvolver um protocolo de conserva??o in vitro de Amburana cearensis. No primeiro cap?tulo, para a multiplica??o in vitro, foram utilizados diferentes reguladores vegetais (BAP, ANA, AIA, KIN) e explantes (embri?o zig?tico e segmentos nodal e cotiledonar; na fase de enraizamento foram testadas as auxinas AIA, AIB e ANA e combina??es de AIB com carv?o ativado. No segundo cap?tulo, foi avaliado o efeito dos agentes osm?ticos sacarose, sorbitol e manitol e dos retardantes de crescimento paclobutrazol e ancymidol na conserva??o in vitro de A. cearensis. O meio utilizado nos experimentos foi o WPM acrescido de 87,64mM de sacarose, exceto nos tratamentos utilizando agente osm?tico que continham varia??es na concentra??o de sacarose. O melhor resultado para multiplica??o foi obtida utilizando o segmento nodal em meios de cultivo acrescido de 1?M de BAP e 0,05 ?M ANA. A adi??o de 2mM de carv?o ativado associado a 4?M de AIB proporcionou as melhores porcentagens de enraizamento dos brotos (54%). A suplementa??o de 262,92?M de sacarose ao meio de cultivo possibilitou a conserva??o de plantas de A. cearensis por 300 dias com 77,5% de sobreviv?ncia.Submitted by Carolina Neves (carolinapon@uefs.br) on 2020-01-09T22:24:11Z No. of bitstreams: 1 _DISSERTA??O Final Bruno Alvim pdf.pdf: 1372502 bytes, checksum: 4df2e1e76aa618fcd7b536fad128c4d7 (MD5)Made available in DSpace on 2020-01-09T22:24:11Z (GMT). No. of bitstreams: 1 _DISSERTA??O Final Bruno Alvim pdf.pdf: 1372502 bytes, checksum: 4df2e1e76aa618fcd7b536fad128c4d7 (MD5) Previous issue date: 2012-05-28Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.uefs.br:8080/retrieve/6285/_DISSERTA%c3%87%c3%83O%20Final%20Bruno%20Alvim%20%20pdf.pdf.jpgporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em Recursos Gen?ticos VegetaisUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASReguladores de crescimentoCarv?o ativadoAgente osm?ticoLeguminosaeGrowth RegulatorsActivated CharcoalOsmotic AgentCIENCIAS AGRARIASMultiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. 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| dc.title.por.fl_str_mv |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| title |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| spellingShingle |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith Alvim, Bruno Freitas Matos Reguladores de crescimento Carv?o ativado Agente osm?tico Leguminosae Growth Regulators Activated Charcoal Osmotic Agent CIENCIAS AGRARIAS |
| title_short |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| title_full |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| title_fullStr |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| title_full_unstemmed |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| title_sort |
Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith |
| author |
Alvim, Bruno Freitas Matos |
| author_facet |
Alvim, Bruno Freitas Matos |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Santana, Jos? Raniere Ferreira de |
| dc.contributor.advisor1ID.fl_str_mv |
55155448549 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1799013075616667 |
| dc.contributor.author.fl_str_mv |
Alvim, Bruno Freitas Matos |
| contributor_str_mv |
Santana, Jos? Raniere Ferreira de |
| dc.subject.por.fl_str_mv |
Reguladores de crescimento Carv?o ativado Agente osm?tico Leguminosae |
| topic |
Reguladores de crescimento Carv?o ativado Agente osm?tico Leguminosae Growth Regulators Activated Charcoal Osmotic Agent CIENCIAS AGRARIAS |
| dc.subject.eng.fl_str_mv |
Growth Regulators Activated Charcoal Osmotic Agent |
| dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
| description |
Amburana cearensis AC Smith, popularly known as "imburana?de-cheiro", "amburana-de-cheiro" and "cumaru", has wide distribution in regions of Caatinga and Cerrado. It stands out due to its multitude of uses, such as forage, food, timber and medicinal. By virtue of degradation of their natural habitats and predatory exploitation, it is listed as endangered species. The aim this study was to optimize the micropropagation protocol and develop a protocol for in vitro conservation Amburana cearensis. In the first chapter, for in vitro multiplication, were used different plant regulators (BAP, ANA, AIA, KIN) and explants (zygotic embryo, nodal and cotyledonary segments; in stage of rooting were tested auxins AIA, AIB, ANA and AIB combination with activated carbon . In the second chapter, was evaluated the effect of osmotic agents sucrose, sorbitol and mannitol and growth retardants paclobutrazol and ancymidol on vitro conservation of A. cearensis. The medium used in the experiments was the WPM supplemented with 87.64 mM sucrose, except in treatments using osmotic agent that contained variations in the concentration of sucrose. The best result was achieved for multiplication using nodal segments in culture environment added with1?M BAP and 0.05 ?M ANA. The addition of 2 mM activated carbon associated with4?M AIB gave the best rooting percentages of shoots (54%). Supplementation of 262.92mM sucrose in the environment enabled the preservation of plants A. cearensis for 300 days with 77.5% survival. |
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2012 |
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2012-05-28 |
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2020-01-09T22:24:11Z |
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ALVIM, Bruno Freitas Matos. Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith. 2012. 84 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
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http://tede2.uefs.br:8080/handle/tede/951 |
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ALVIM, Bruno Freitas Matos. Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith. 2012. 84 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012. |
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