Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Alvim, Bruno Freitas Matos lattes
Orientador(a): Santana, Jos? Raniere Ferreira de
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Feira de Santana
Programa de Pós-Graduação: Mestrado Acad?mico em Recursos Gen?ticos Vegetais
Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede2.uefs.br:8080/handle/tede/951
Resumo: Amburana cearensis AC Smith, popularly known as "imburana?de-cheiro", "amburana-de-cheiro" and "cumaru", has wide distribution in regions of Caatinga and Cerrado. It stands out due to its multitude of uses, such as forage, food, timber and medicinal. By virtue of degradation of their natural habitats and predatory exploitation, it is listed as endangered species. The aim this study was to optimize the micropropagation protocol and develop a protocol for in vitro conservation Amburana cearensis. In the first chapter, for in vitro multiplication, were used different plant regulators (BAP, ANA, AIA, KIN) and explants (zygotic embryo, nodal and cotyledonary segments; in stage of rooting were tested auxins AIA, AIB, ANA and AIB combination with activated carbon . In the second chapter, was evaluated the effect of osmotic agents sucrose, sorbitol and mannitol and growth retardants paclobutrazol and ancymidol on vitro conservation of A. cearensis. The medium used in the experiments was the WPM supplemented with 87.64 mM sucrose, except in treatments using osmotic agent that contained variations in the concentration of sucrose. The best result was achieved for multiplication using nodal segments in culture environment added with1?M BAP and 0.05 ?M ANA. The addition of 2 mM activated carbon associated with4?M AIB gave the best rooting percentages of shoots (54%). Supplementation of 262.92mM sucrose in the environment enabled the preservation of plants A. cearensis for 300 days with 77.5% survival.
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spelling Santana, Jos? Raniere Ferreira de55155448549http://lattes.cnpq.br/1799013075616667Alvim, Bruno Freitas Matos2020-01-09T22:24:11Z2012-05-28ALVIM, Bruno Freitas Matos. Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith. 2012. 84 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.http://tede2.uefs.br:8080/handle/tede/951Amburana cearensis AC Smith, popularly known as "imburana?de-cheiro", "amburana-de-cheiro" and "cumaru", has wide distribution in regions of Caatinga and Cerrado. It stands out due to its multitude of uses, such as forage, food, timber and medicinal. By virtue of degradation of their natural habitats and predatory exploitation, it is listed as endangered species. The aim this study was to optimize the micropropagation protocol and develop a protocol for in vitro conservation Amburana cearensis. In the first chapter, for in vitro multiplication, were used different plant regulators (BAP, ANA, AIA, KIN) and explants (zygotic embryo, nodal and cotyledonary segments; in stage of rooting were tested auxins AIA, AIB, ANA and AIB combination with activated carbon . In the second chapter, was evaluated the effect of osmotic agents sucrose, sorbitol and mannitol and growth retardants paclobutrazol and ancymidol on vitro conservation of A. cearensis. The medium used in the experiments was the WPM supplemented with 87.64 mM sucrose, except in treatments using osmotic agent that contained variations in the concentration of sucrose. The best result was achieved for multiplication using nodal segments in culture environment added with1?M BAP and 0.05 ?M ANA. The addition of 2 mM activated carbon associated with4?M AIB gave the best rooting percentages of shoots (54%). Supplementation of 262.92mM sucrose in the environment enabled the preservation of plants A. cearensis for 300 days with 77.5% survival.Amburana cearensis A.C. Smith, conhecida popularmente como imburana-de-cheiro, amburana-de-cheiro e cumaru, tem ampla distribui??o nas regi?es de Caatinga e Cerrado. Destaca-se devido a sua multiplicidade de usos, tais como forrageiro, alimentar, madeireiro e medicinal. Em virtude da degrada??o de seus h?bitats naturais e sua explora??o predat?ria, ? listada como esp?cie amea?ada de extin??o. O objetivo deste estudo foi otimizar o protocolo de micropropaga??o e desenvolver um protocolo de conserva??o in vitro de Amburana cearensis. No primeiro cap?tulo, para a multiplica??o in vitro, foram utilizados diferentes reguladores vegetais (BAP, ANA, AIA, KIN) e explantes (embri?o zig?tico e segmentos nodal e cotiledonar; na fase de enraizamento foram testadas as auxinas AIA, AIB e ANA e combina??es de AIB com carv?o ativado. No segundo cap?tulo, foi avaliado o efeito dos agentes osm?ticos sacarose, sorbitol e manitol e dos retardantes de crescimento paclobutrazol e ancymidol na conserva??o in vitro de A. cearensis. O meio utilizado nos experimentos foi o WPM acrescido de 87,64mM de sacarose, exceto nos tratamentos utilizando agente osm?tico que continham varia??es na concentra??o de sacarose. O melhor resultado para multiplica??o foi obtida utilizando o segmento nodal em meios de cultivo acrescido de 1?M de BAP e 0,05 ?M ANA. A adi??o de 2mM de carv?o ativado associado a 4?M de AIB proporcionou as melhores porcentagens de enraizamento dos brotos (54%). A suplementa??o de 262,92?M de sacarose ao meio de cultivo possibilitou a conserva??o de plantas de A. cearensis por 300 dias com 77,5% de sobreviv?ncia.Submitted by Carolina Neves (carolinapon@uefs.br) on 2020-01-09T22:24:11Z No. of bitstreams: 1 _DISSERTA??O Final Bruno Alvim pdf.pdf: 1372502 bytes, checksum: 4df2e1e76aa618fcd7b536fad128c4d7 (MD5)Made available in DSpace on 2020-01-09T22:24:11Z (GMT). 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dc.title.por.fl_str_mv Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
title Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
spellingShingle Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
Alvim, Bruno Freitas Matos
Reguladores de crescimento
Carv?o ativado
Agente osm?tico
Leguminosae
Growth Regulators
Activated Charcoal
Osmotic Agent
CIENCIAS AGRARIAS
title_short Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
title_full Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
title_fullStr Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
title_full_unstemmed Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
title_sort Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith
author Alvim, Bruno Freitas Matos
author_facet Alvim, Bruno Freitas Matos
author_role author
dc.contributor.advisor1.fl_str_mv Santana, Jos? Raniere Ferreira de
dc.contributor.advisor1ID.fl_str_mv 55155448549
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1799013075616667
dc.contributor.author.fl_str_mv Alvim, Bruno Freitas Matos
contributor_str_mv Santana, Jos? Raniere Ferreira de
dc.subject.por.fl_str_mv Reguladores de crescimento
Carv?o ativado
Agente osm?tico
Leguminosae
topic Reguladores de crescimento
Carv?o ativado
Agente osm?tico
Leguminosae
Growth Regulators
Activated Charcoal
Osmotic Agent
CIENCIAS AGRARIAS
dc.subject.eng.fl_str_mv Growth Regulators
Activated Charcoal
Osmotic Agent
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS
description Amburana cearensis AC Smith, popularly known as "imburana?de-cheiro", "amburana-de-cheiro" and "cumaru", has wide distribution in regions of Caatinga and Cerrado. It stands out due to its multitude of uses, such as forage, food, timber and medicinal. By virtue of degradation of their natural habitats and predatory exploitation, it is listed as endangered species. The aim this study was to optimize the micropropagation protocol and develop a protocol for in vitro conservation Amburana cearensis. In the first chapter, for in vitro multiplication, were used different plant regulators (BAP, ANA, AIA, KIN) and explants (zygotic embryo, nodal and cotyledonary segments; in stage of rooting were tested auxins AIA, AIB, ANA and AIB combination with activated carbon . In the second chapter, was evaluated the effect of osmotic agents sucrose, sorbitol and mannitol and growth retardants paclobutrazol and ancymidol on vitro conservation of A. cearensis. The medium used in the experiments was the WPM supplemented with 87.64 mM sucrose, except in treatments using osmotic agent that contained variations in the concentration of sucrose. The best result was achieved for multiplication using nodal segments in culture environment added with1?M BAP and 0.05 ?M ANA. The addition of 2 mM activated carbon associated with4?M AIB gave the best rooting percentages of shoots (54%). Supplementation of 262.92mM sucrose in the environment enabled the preservation of plants A. cearensis for 300 days with 77.5% survival.
publishDate 2012
dc.date.issued.fl_str_mv 2012-05-28
dc.date.accessioned.fl_str_mv 2020-01-09T22:24:11Z
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dc.identifier.citation.fl_str_mv ALVIM, Bruno Freitas Matos. Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith. 2012. 84 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/951
identifier_str_mv ALVIM, Bruno Freitas Matos. Multiplica??o e conserva??o in vitro de Amburana cearensis (Allem?o) A. C. Smith. 2012. 84 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2012.
url http://tede2.uefs.br:8080/handle/tede/951
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