Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o
Ano de defesa: | 2017 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Biotecnologia
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/839 |
Resumo: | Bananas are one of the most important fruits worldwide since the attributes of color, flavor and texture contribute to its popularity. Brazil is among the four main banana producers, together with India, China and the Philippines, with approximately 7 million tons produced in a cultivated area of 517 thousand hectares. In the international market, cultivars of the Cavendish subgroup are more exported and responsible for approximately 50% of the world fruit market. In Brazil, bananas from the Prata-type subgroup occupy approximately 60% of the cultivated area, with great acceptance in the internal market due to its flavor and aroma. After harvested, fruits undergo many physiological and biochemical alterations due to ripening. However, different edaphoclimatic conditions may alter organoleptic characteristics of the fruit and influence the entire ripening process. Therefore, the objective of the present work was to evaluate Prata-An? fruits grown in two different growing periods (2013 and 2015) in order to verify the influence of the edaphoclimatic conditions in the final quality of fruits. Secondly, genes involved in the ripening process were selected from the first Musa spp. interatome. Post-harvest evaluations of Prata-An? fruits indicated that fruits developed under climatic conditions of period 1, presented superior fruits as to length, diameter, color and sweetness, in comparison to period II. According to market rules, fruits from period I and II were classified as first and second Prata, respectively. Proteomic analysis with fruits from period I in stages of maturation 1,4 and 6, identified 221 proteins, whereas 36 were exclusive of the green fruits (E1), 14 of the intermediary fruits (E4), 39 of the ripened fruits (E6) and 124 differentially expressed. The first interatome network of Musa spp. was carried out using the protein profile of the ripening stages with important groups and functions such as protein catabolism and metabolism of carbohydrates with the identification of key proteins involved in the ripening process as well as the corresponding genes validated by qRT-PCR. Results may lead to search of new approaches to explore means of control of banana fruit development and ripening |
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Amorim, Edson Peritohttp://lattes.cnpq.br/672667530570634100249682346http://lattes.cnpq.br/2314433428927071Roque, Rafaella de Lima2019-08-14T22:57:17Z2017-05-02ROQUE, Rafaella de Lima. Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o. 2017. 125f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017.http://tede2.uefs.br:8080/handle/tede/839Bananas are one of the most important fruits worldwide since the attributes of color, flavor and texture contribute to its popularity. Brazil is among the four main banana producers, together with India, China and the Philippines, with approximately 7 million tons produced in a cultivated area of 517 thousand hectares. In the international market, cultivars of the Cavendish subgroup are more exported and responsible for approximately 50% of the world fruit market. In Brazil, bananas from the Prata-type subgroup occupy approximately 60% of the cultivated area, with great acceptance in the internal market due to its flavor and aroma. After harvested, fruits undergo many physiological and biochemical alterations due to ripening. However, different edaphoclimatic conditions may alter organoleptic characteristics of the fruit and influence the entire ripening process. Therefore, the objective of the present work was to evaluate Prata-An? fruits grown in two different growing periods (2013 and 2015) in order to verify the influence of the edaphoclimatic conditions in the final quality of fruits. Secondly, genes involved in the ripening process were selected from the first Musa spp. interatome. Post-harvest evaluations of Prata-An? fruits indicated that fruits developed under climatic conditions of period 1, presented superior fruits as to length, diameter, color and sweetness, in comparison to period II. According to market rules, fruits from period I and II were classified as first and second Prata, respectively. Proteomic analysis with fruits from period I in stages of maturation 1,4 and 6, identified 221 proteins, whereas 36 were exclusive of the green fruits (E1), 14 of the intermediary fruits (E4), 39 of the ripened fruits (E6) and 124 differentially expressed. The first interatome network of Musa spp. was carried out using the protein profile of the ripening stages with important groups and functions such as protein catabolism and metabolism of carbohydrates with the identification of key proteins involved in the ripening process as well as the corresponding genes validated by qRT-PCR. Results may lead to search of new approaches to explore means of control of banana fruit development and ripeningA banana ? uma das frutas mais importantes do mundo uma vez que atributos como cor, sabor e textura, contribuem para a sua popularidade. O Brasil destaca-se mundialmente situado entre os quatro maiores produtores, juntamente com ?ndia, China e Filipinas, com aproximadamente 7,0 milh?es de toneladas produzidas, cultivadas em uma ?rea de 517 mil hectares. Internacionalmente, as cultivares do subgrupo Cavendish s?o as mais difundidas, sendo consideradas tipo exporta??o e respons?veis por aproximadamente 50% do com?rcio mundial da fruta. No Brasil, predomina o cultivo do subgrupo Prata, que ocupa aproximadamente 60% da ?rea cultivada, em especial devido ? sua boa aceita??o pelo mercado interno em fun??o de caracter?sticas como sabor e aroma. Ap?s a colheita, os frutos passam por v?rias altera??es fisiol?gicas e bioqu?micas decorrentes do processo de matura??o, entretanto diferentes condi??es clim?ticas podem alterar as caracter?sticas organol?pticas do fruto e influenciar todo o processo de matura??o. Dessa forma, objetivou-se com esse estudo, avaliar frutos de ?Prata-An?? desenvolvidos em duas ?pocas distintas (2013 e 2015) para verificar a influ?ncia das condi??es clim?ticas na qualidade final dos frutos, bem como identificar o perfil proteico dos est?dios 1 4 e 6 de matura??o e selecionar genes envolvidos no processo de amadurecimento, a partir da constru??o do primeiro interatoma de Musa spp. De acordo com normas de mercado, os frutos da ?poca I e II foram classificados como Prata de primeira e segunda, respectivamente. As avalia??es p?s-colheita dos frutos de ?Prata-An?? indicaram que os frutos desenvolvidos nas condi??es clim?ticas da ?poca I apresentaram caracter?sticas como comprimento, di?metro, cor e do?ura dos frutos superiores aos frutos da ?poca II. A an?lise prote?mica realizada com frutos da ?poca I dos est?dios de matura??o 1, 4 e 6, permitiu a identifica??o de 221 prote?nas, sendo 36 exclusivas do fruto verde (E1), 14 do fruto intermedi?rio (E4), 39 do fruto maduro (E6) e 124 diferencialmente expressas. O perfil proteico dos est?dios de matura??o permitiu a constru??o da primeira rede de intera??es de Musa spp., com a forma??o de grupos de fun??es importantes como catabolismo proteico e metabolismo de carboidratos, possibilitando a identifica??o de prote?nas chave envolvidas no processo do amadurecimento, bem como o reconhecimento dos genes correspondentes, verificados por meio do qRT-PCR. Os resultados obtidos nessa pesquisa podem levar a busca por a novas abordagens para explorar vias de controle de desenvolvimento e da matura??o de frutos de bananeiraSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2019-08-14T22:57:17Z No. of bitstreams: 1 TESE_VERS?O FINAL_RAFAELLA DE LIMA ROQUE.pdf: 2372929 bytes, checksum: cf80263c24799933ef910b8cfa02b996 (MD5)Made available in DSpace on 2019-08-14T22:57:17Z (GMT). No. of bitstreams: 1 TESE_VERS?O FINAL_RAFAELLA DE LIMA ROQUE.pdf: 2372929 bytes, checksum: cf80263c24799933ef910b8cfa02b996 (MD5) Previous issue date: 2017-05-02Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEBapplication/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASMusa spp.qualidadecondi??es clim?ticasinteratomagenesMusa spp.qualityedaphoclimatic conditionsinteratomegenesCIENCIAS BIOLOGICAS::GENETICACIENCIAS BIOLOGICASEstudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??oinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-68152692297897915436006006006006005026123383450589282-5518144268585252051-3439178843068202161-8233071094704392586info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTESE_VERS?O FINAL_RAFAELLA DE LIMA ROQUE.pdfTESE_VERS?O FINAL_RAFAELLA DE LIMA ROQUE.pdfapplication/pdf2372929http://tede2.uefs.br:8080/bitstream/tede/839/2/TESE_VERS%C3%83O+FINAL_RAFAELLA+DE+LIMA+ROQUE.pdfcf80263c24799933ef910b8cfa02b996MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/839/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/8392019-08-14 19:57:17.974oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-08-14T22:57:17Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
title |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
spellingShingle |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o Roque, Rafaella de Lima Musa spp. qualidade condi??es clim?ticas interatoma genes Musa spp. quality edaphoclimatic conditions interatome genes CIENCIAS BIOLOGICAS::GENETICA CIENCIAS BIOLOGICAS |
title_short |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
title_full |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
title_fullStr |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
title_full_unstemmed |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
title_sort |
Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o |
author |
Roque, Rafaella de Lima |
author_facet |
Roque, Rafaella de Lima |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Amorim, Edson Perito |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6726675305706341 |
dc.contributor.authorID.fl_str_mv |
00249682346 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2314433428927071 |
dc.contributor.author.fl_str_mv |
Roque, Rafaella de Lima |
contributor_str_mv |
Amorim, Edson Perito |
dc.subject.por.fl_str_mv |
Musa spp. qualidade condi??es clim?ticas interatoma genes |
topic |
Musa spp. qualidade condi??es clim?ticas interatoma genes Musa spp. quality edaphoclimatic conditions interatome genes CIENCIAS BIOLOGICAS::GENETICA CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Musa spp. quality edaphoclimatic conditions interatome genes |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA CIENCIAS BIOLOGICAS |
description |
Bananas are one of the most important fruits worldwide since the attributes of color, flavor and texture contribute to its popularity. Brazil is among the four main banana producers, together with India, China and the Philippines, with approximately 7 million tons produced in a cultivated area of 517 thousand hectares. In the international market, cultivars of the Cavendish subgroup are more exported and responsible for approximately 50% of the world fruit market. In Brazil, bananas from the Prata-type subgroup occupy approximately 60% of the cultivated area, with great acceptance in the internal market due to its flavor and aroma. After harvested, fruits undergo many physiological and biochemical alterations due to ripening. However, different edaphoclimatic conditions may alter organoleptic characteristics of the fruit and influence the entire ripening process. Therefore, the objective of the present work was to evaluate Prata-An? fruits grown in two different growing periods (2013 and 2015) in order to verify the influence of the edaphoclimatic conditions in the final quality of fruits. Secondly, genes involved in the ripening process were selected from the first Musa spp. interatome. Post-harvest evaluations of Prata-An? fruits indicated that fruits developed under climatic conditions of period 1, presented superior fruits as to length, diameter, color and sweetness, in comparison to period II. According to market rules, fruits from period I and II were classified as first and second Prata, respectively. Proteomic analysis with fruits from period I in stages of maturation 1,4 and 6, identified 221 proteins, whereas 36 were exclusive of the green fruits (E1), 14 of the intermediary fruits (E4), 39 of the ripened fruits (E6) and 124 differentially expressed. The first interatome network of Musa spp. was carried out using the protein profile of the ripening stages with important groups and functions such as protein catabolism and metabolism of carbohydrates with the identification of key proteins involved in the ripening process as well as the corresponding genes validated by qRT-PCR. Results may lead to search of new approaches to explore means of control of banana fruit development and ripening |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-05-02 |
dc.date.accessioned.fl_str_mv |
2019-08-14T22:57:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
ROQUE, Rafaella de Lima. Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o. 2017. 125f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/839 |
identifier_str_mv |
ROQUE, Rafaella de Lima. Estudos de prote?mica em frutos de bananeira da cultivar prata-an? durante o processo de matura??o. 2017. 125f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017. |
url |
http://tede2.uefs.br:8080/handle/tede/839 |
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openAccess |
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Universidade Estadual de Feira de Santana |
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UEFS |
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DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
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Universidade Estadual de Feira de Santana |
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