Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad

Detalhes bibliográficos
Ano de defesa: 2011
Autor(a) principal: Ribeiro, Fernanda Pinheiro Carvalho lattes
Orientador(a): Branco, Alexsandro
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Feira de Santana
Programa de Pós-Graduação: Mestrado Acad?mico em Recursos Gen?ticos Vegetais
Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
País: Brasil
Palavras-chave em Português:
Planta medicinal
Mikania laevigata
Metab?litos secund?rios
Palavras-chave em Inglês:
Medicinal plants
Secondary metabolites
Área do conhecimento CNPq:
CIENCIAS AGRARIAS
Link de acesso: http://tede2.uefs.br:8080/handle/tede/1106
Resumo: The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate.
id UEFS_ccf56d900a40e144a9fec8182653cd45
oai_identifier_str oai:tede2.uefs.br:8080:tede/1106
network_acronym_str UEFS
network_name_str Biblioteca Digital de Teses e Dissertações da UEFS
repository_id_str
spelling Branco, Alexsandro9234396197293536747515http://lattes.cnpq.br/3497222662010996Ribeiro, Fernanda Pinheiro Carvalho2020-05-08T00:11:03Z2011-08-12RIBEIRO, Fernanda Pinheiro de Carvalho. Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad. 2011. 139 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.http://tede2.uefs.br:8080/handle/tede/1106The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate.As intera??es entre fungos e plantas, especialmente fungos endof?ticos, s?o consideradas importantes fontes geradoras de metab?litos secund?rios. A detec??o destes microrganismos no interior de plantas sadias abriu novas perspectivas para o estudo das intera??es fungos-planta. O presente trabalho teve como objetivo isolar e identificar por an?lise molecular os fungos endof?ticos de Mikania laevigata (Asteraceae) e realizar a an?lise qu?mica por CLAE-DAD dos extratos obtidos destes fungos. As folhas de Mikania laevigata, cultivadas em horto florestal, foram submetidas a procedimento de isolamento dos fungos endof?ticos que resultou em 40 isolados. Destes, 15 foram selecionados e caracterizados pelos aspectos morfol?gicos como ascomicetos da fam?lia Xylariaceae. A investiga??o molecular atrav?s do sequenciamento da regi?o ITS rDNA resultou na identifica??o de 3 cepas de Nodulisporium sp., 3 cepas de Hypoxylon sp., 1 cepa de Daldinia sp. e 1 esp?cie identificada como Xylaria luteostromata. N?o foi poss?vel identificar em n?vel molecular as demais amostras submetidas ao seq?enciamento. A an?lise por CLAE-DAD revelou grande diversidade qu?mica entre os extratos analisados. Notou-se a presen?a de uma maior quantidade de metab?litos nos extratos externos em compara??o com os extratos micelares. Os extratos metan?licos mostraram a presen?a de um maior n?mero de metab?litos quando comparados aos extratos em acetato de etila.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2020-05-08T00:11:03Z No. of bitstreams: 1 Monografia.pdf: 3369567 bytes, checksum: 076a115698fc0d6aaa3cb19b76bae2c9 (MD5)Made available in DSpace on 2020-05-08T00:11:03Z (GMT). No. of bitstreams: 1 Monografia.pdf: 3369567 bytes, checksum: 076a115698fc0d6aaa3cb19b76bae2c9 (MD5) Previous issue date: 2011-08-12application/pdfhttp://tede2.uefs.br:8080/retrieve/6575/Monografia.pdf.jpgporUniversidade Estadual de Feira de SantanaMestrado Acad?mico em Recursos Gen?ticos VegetaisUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASPlanta medicinalMikania laevigataMetab?litos secund?riosMedicinal plantsSecondary metabolitesCIENCIAS AGRARIASIsolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dadinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis814664648162224187460060060050261233834505892827828424726906663919info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSTHUMBNAILMonografia.pdf.jpgMonografia.pdf.jpgimage/jpeg2236http://tede2.uefs.br:8080/bitstream/tede/1106/4/Monografia.pdf.jpgc1859d2c848fdf90c14f03f305a67926MD54TEXTMonografia.pdf.txtMonografia.pdf.txttext/plain129626http://tede2.uefs.br:8080/bitstream/tede/1106/3/Monografia.pdf.txt82ff908b66d4ea3a46d5d91859b5a354MD53ORIGINALMonografia.pdfMonografia.pdfapplication/pdf3369567http://tede2.uefs.br:8080/bitstream/tede/1106/2/Monografia.pdf076a115698fc0d6aaa3cb19b76bae2c9MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/1106/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/11062025-09-10 01:24:27.866oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2025-09-10T04:24:27Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false
dc.title.por.fl_str_mv Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
title Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
spellingShingle Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
Ribeiro, Fernanda Pinheiro Carvalho
Planta medicinal
Mikania laevigata
Metab?litos secund?rios
Medicinal plants
Secondary metabolites
CIENCIAS AGRARIAS
title_short Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
title_full Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
title_fullStr Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
title_full_unstemmed Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
title_sort Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad
author Ribeiro, Fernanda Pinheiro Carvalho
author_facet Ribeiro, Fernanda Pinheiro Carvalho
author_role author
dc.contributor.advisor1.fl_str_mv Branco, Alexsandro
dc.contributor.advisor1ID.fl_str_mv 92343961972
dc.contributor.authorID.fl_str_mv 93536747515
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3497222662010996
dc.contributor.author.fl_str_mv Ribeiro, Fernanda Pinheiro Carvalho
contributor_str_mv Branco, Alexsandro
dc.subject.por.fl_str_mv Planta medicinal
Mikania laevigata
Metab?litos secund?rios
topic Planta medicinal
Mikania laevigata
Metab?litos secund?rios
Medicinal plants
Secondary metabolites
CIENCIAS AGRARIAS
dc.subject.eng.fl_str_mv Medicinal plants
Secondary metabolites
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS
description The interactions between fungi and plants, especially endophytic fungi, are considered important sources of secondary metabolites. The detection of these microorganisms inside healthy plants has opened new perspectives for the study of fungus-plant interactions. This study aimed to isolate and identify the molecular analysis of endophytic fungi of Mikania laevigata (Asteraceae) and perform chemical analysis by HPLC-DAD extracts of these fungi. The leaves of Mikania laevigata grown in tree farm were subjected to the isolation procedure that resulted in endophytic fungi isolated 40. Of these, 15 were selected and characterized by morphological features as Xylariaceae ascomycetes family. Molecular investigation by sequencing the rDNA ITS region resulted in the identification of three strains of Nodulisporium sp., 3 strains of Hypoxylon sp. Daldinia sp 1 strain. and a species identified as Xylaria luteostromata. Could not be identified at the molecular level the other samples subjected to sequencing. The analysis by HPLC-DAD showed a large chemical diversity among the analyzed extracts. It was noted the presence of a greater amount of metabolites in the extracts compared to the external micellar extracts. The methanol extracts showed the presence of a greater number of metabolites when compared to extracts in ethyl acetate.
publishDate 2011
dc.date.issued.fl_str_mv 2011-08-12
dc.date.accessioned.fl_str_mv 2020-05-08T00:11:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Fernanda Pinheiro de Carvalho. Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad. 2011. 139 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/1106
identifier_str_mv RIBEIRO, Fernanda Pinheiro de Carvalho. Isolamento e identifica??o de fungos endofiticos de Mikania laevigata (asteroceae) e an?lise qu?mica dos extratos por clae-dad. 2011. 139 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais)- Universidade Estadual de Feira de Santana, Feira de Santana, 2011.
url http://tede2.uefs.br:8080/handle/tede/1106
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 8146646481622241874
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv 5026123383450589282
dc.relation.cnpq.fl_str_mv 7828424726906663919
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
dc.publisher.program.fl_str_mv Mestrado Acad?mico em Recursos Gen?ticos Vegetais
dc.publisher.initials.fl_str_mv UEFS
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UEFS
instname:Universidade Estadual de Feira de Santana (UEFS)
instacron:UEFS
instname_str Universidade Estadual de Feira de Santana (UEFS)
instacron_str UEFS
institution UEFS
reponame_str Biblioteca Digital de Teses e Dissertações da UEFS
collection Biblioteca Digital de Teses e Dissertações da UEFS
bitstream.url.fl_str_mv http://tede2.uefs.br:8080/bitstream/tede/1106/4/Monografia.pdf.jpg
http://tede2.uefs.br:8080/bitstream/tede/1106/3/Monografia.pdf.txt
http://tede2.uefs.br:8080/bitstream/tede/1106/2/Monografia.pdf
http://tede2.uefs.br:8080/bitstream/tede/1106/1/license.txt
bitstream.checksum.fl_str_mv c1859d2c848fdf90c14f03f305a67926
82ff908b66d4ea3a46d5d91859b5a354
076a115698fc0d6aaa3cb19b76bae2c9
7b5ba3d2445355f386edab96125d42b7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)
repository.mail.fl_str_mv bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br
_version_ 1845618194470928384

Registros relacionados