Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Silva, Gabriela Carinhanha lattes
Orientador(a): Lucchese, Ang?lica Maria lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Feira de Santana
Programa de Pós-Graduação: Doutorado Acad?mico em Recursos Gen?ticos Vegetais
Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede2.uefs.br:8080/handle/tede/741
Resumo: Lippia origanoides, native medicinal aromatic shrub, has broad range of biologic activities. Elicitation, stimulation of plant defense, aims to rise the pruction of active compounds. Thus, it aimed to investigate the production, composition and activity of metabolites from this specie under elicitation. Cultivated plants were sprayed with cell material suspension of Aspergillus niger (AN) and Fusarium oxysporum (FO), solution of hydrogen peroxide (SH2O2), and distilled water. Hydrodistilled essential oils (EO) from leaves (LEO) and from inflorescences (IEO) were quantified, analyzed by GC/MS and tested against microorganisms by broth microdilution. The content of total phenolics calculated asgallic acid equivalent and flavonoids calculated asquercetin equivalent, and free radical scavenging activity (FRSA) using DPPH? of methanolic extracts (ME) of the leaves, inflorescences, stem and root were measured by spectrophotometer. It was calculated the in vitro solar protection factor (SPF) of ME and of photoprotective formulations (PPF) with the more active extract by spectrophotometer. SH2O2 increased the IEO production. FO increased the bicyclogermacrene concentration on IEO. All elicitors increase the effectiveness of both EO against Candida albicans and Staphylococcus aureus, and to reduce the activity against Escherichia coli of these one. FO and SH2O2 reduced the phenolic compounds on ME of stem and root. FO reduced the content of flavonoids on ME of the root. FO increased the FRSA of ME of stem. SH2O2 and AN reduced the FRSA of ME of root. SH2O2 increased the SPF of ME of stem and inflorescence. The ME of inflorescence treated with SH2O2 raised the SPF of PPF containing synthetic active compounds. It is concluded that FO and SH2O2 have potentiality as elicitors of L. origanoides active compounds production
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spelling Lucchese, Ang?lica Maria39684639091http://lattes.cnpq.br/584045281802431303017649563http://lattes.cnpq.br/8953930003176411Silva, Gabriela Carinhanha2019-04-23T23:51:43Z2017-09-25SILVA, Gabriela Carinhanha. Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae). 2017. 114f. Tese (Doutorado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017.http://tede2.uefs.br:8080/handle/tede/741Lippia origanoides, native medicinal aromatic shrub, has broad range of biologic activities. Elicitation, stimulation of plant defense, aims to rise the pruction of active compounds. Thus, it aimed to investigate the production, composition and activity of metabolites from this specie under elicitation. Cultivated plants were sprayed with cell material suspension of Aspergillus niger (AN) and Fusarium oxysporum (FO), solution of hydrogen peroxide (SH2O2), and distilled water. Hydrodistilled essential oils (EO) from leaves (LEO) and from inflorescences (IEO) were quantified, analyzed by GC/MS and tested against microorganisms by broth microdilution. The content of total phenolics calculated asgallic acid equivalent and flavonoids calculated asquercetin equivalent, and free radical scavenging activity (FRSA) using DPPH? of methanolic extracts (ME) of the leaves, inflorescences, stem and root were measured by spectrophotometer. It was calculated the in vitro solar protection factor (SPF) of ME and of photoprotective formulations (PPF) with the more active extract by spectrophotometer. SH2O2 increased the IEO production. FO increased the bicyclogermacrene concentration on IEO. All elicitors increase the effectiveness of both EO against Candida albicans and Staphylococcus aureus, and to reduce the activity against Escherichia coli of these one. FO and SH2O2 reduced the phenolic compounds on ME of stem and root. FO reduced the content of flavonoids on ME of the root. FO increased the FRSA of ME of stem. SH2O2 and AN reduced the FRSA of ME of root. SH2O2 increased the SPF of ME of stem and inflorescence. The ME of inflorescence treated with SH2O2 raised the SPF of PPF containing synthetic active compounds. It is concluded that FO and SH2O2 have potentiality as elicitors of L. origanoides active compounds productionLippia origanoides, arbusto arom?tico medicinal nativo, possui ampla gama de atividades biol?gicas, como antioxidante e antimicrobiana. A elicita??o, estimula??o da defesa vegetal, visa aumentar a produ??o compostos ativos. Assim, objetivou-se investigar a produ??o, composi??o e atividades antimicrobiana, antioxidante e fotoprotetora de metab?litos desta esp?cie sob elicita??o. Plantas cultivadas foram pulverizadas com suspens?es de material celular de Aspergillus niger (AN) e Fusarium oxysporum (FO), solu??o de per?xido de hidrog?nio (SH2O2) e ?gua destilada. ?leos essenciais (OE?s) hidrodestilados de folhas (OEF) e de infloresc?ncias (OEI) foram quantificados, analisados por CG/EM e testados contra micro-organismos em microdilui??o em caldo. Os teores de fen?licos, equivalentes a ?cido g?lico, e flavonoides totais, equivalentes a quercetina, e Atividade de Sequestro de Radical Livre DPPH? (ASRL) de extratos metan?licos (EM) de folhas, infloresc?ncias, caule e raiz foram mensurados por espectrofot?metro. Obteve-se o Fator de Prote??o Solar in vitro(FPS) e a raz?o UVA/UVB dos EM?s por espectrofot?metro e de formula??es fotoprotetoras (FFP?s) contendo o extrato mais ativo. SH2O2 aumentou a produ??o de OEI. FO elevou a concentra??o de biciclogermacreno no OEI. Todos os elicitores aumentaram a efici?ncia dos OE?s contra Candida albicans e Staphylococcus aureus e reduziram contra Escherichia coli. FO e SH2O2 reduziram os fen?licos nos EM?s de caule e raiz. FO reduziu os flavonoides do ME da raiz. A ASRL do EM do caule aumentou com FO e do EM da raiz reduziu com SH2O2 e AN. SH2O2 aumentou o FPS do EM de caule e infloresc?ncias. O EM de infloresc?ncias tratadas com SH2O2 aumentou o FPS e a raz?o UVA/UVB de FFP?s contendo ativos sint?ticos. Conclui-se que FO e SH2O2 possuem potencial como elicitores da produ??o de compostos ativos em L. origanoidesSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2019-04-23T23:51:43Z No. of bitstreams: 1 Tese_Gabriela_Carinhanha_Silva.pdf: 3353273 bytes, checksum: 707622ed71a813c2e66f4306762948f0 (MD5)Made available in DSpace on 2019-04-23T23:51:43Z (GMT). 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dc.title.por.fl_str_mv Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
title Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
spellingShingle Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
Silva, Gabriela Carinhanha
Alecrim-de-tabuleiro
Estimula??o da defesa vegetal
Produ??o de metab?litos ativos
Antimicrobiano
Antioxidante. Fotoprote??o
Mexican oregano
Plant defense stimulation
Production of active metabolites
Antimicrobial
Antioxidant
Photoprotection
GENETICA::GENETICA VEGETAL
title_short Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
title_full Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
title_fullStr Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
title_full_unstemmed Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
title_sort Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae)
author Silva, Gabriela Carinhanha
author_facet Silva, Gabriela Carinhanha
author_role author
dc.contributor.advisor1.fl_str_mv Lucchese, Ang?lica Maria
dc.contributor.advisor1ID.fl_str_mv 39684639091
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5840452818024313
dc.contributor.authorID.fl_str_mv 03017649563
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8953930003176411
dc.contributor.author.fl_str_mv Silva, Gabriela Carinhanha
contributor_str_mv Lucchese, Ang?lica Maria
dc.subject.por.fl_str_mv Alecrim-de-tabuleiro
Estimula??o da defesa vegetal
Produ??o de metab?litos ativos
Antimicrobiano
Antioxidante. Fotoprote??o
topic Alecrim-de-tabuleiro
Estimula??o da defesa vegetal
Produ??o de metab?litos ativos
Antimicrobiano
Antioxidante. Fotoprote??o
Mexican oregano
Plant defense stimulation
Production of active metabolites
Antimicrobial
Antioxidant
Photoprotection
GENETICA::GENETICA VEGETAL
dc.subject.eng.fl_str_mv Mexican oregano
Plant defense stimulation
Production of active metabolites
Antimicrobial
Antioxidant
Photoprotection
dc.subject.cnpq.fl_str_mv GENETICA::GENETICA VEGETAL
description Lippia origanoides, native medicinal aromatic shrub, has broad range of biologic activities. Elicitation, stimulation of plant defense, aims to rise the pruction of active compounds. Thus, it aimed to investigate the production, composition and activity of metabolites from this specie under elicitation. Cultivated plants were sprayed with cell material suspension of Aspergillus niger (AN) and Fusarium oxysporum (FO), solution of hydrogen peroxide (SH2O2), and distilled water. Hydrodistilled essential oils (EO) from leaves (LEO) and from inflorescences (IEO) were quantified, analyzed by GC/MS and tested against microorganisms by broth microdilution. The content of total phenolics calculated asgallic acid equivalent and flavonoids calculated asquercetin equivalent, and free radical scavenging activity (FRSA) using DPPH? of methanolic extracts (ME) of the leaves, inflorescences, stem and root were measured by spectrophotometer. It was calculated the in vitro solar protection factor (SPF) of ME and of photoprotective formulations (PPF) with the more active extract by spectrophotometer. SH2O2 increased the IEO production. FO increased the bicyclogermacrene concentration on IEO. All elicitors increase the effectiveness of both EO against Candida albicans and Staphylococcus aureus, and to reduce the activity against Escherichia coli of these one. FO and SH2O2 reduced the phenolic compounds on ME of stem and root. FO reduced the content of flavonoids on ME of the root. FO increased the FRSA of ME of stem. SH2O2 and AN reduced the FRSA of ME of root. SH2O2 increased the SPF of ME of stem and inflorescence. The ME of inflorescence treated with SH2O2 raised the SPF of PPF containing synthetic active compounds. It is concluded that FO and SH2O2 have potentiality as elicitors of L. origanoides active compounds production
publishDate 2017
dc.date.issued.fl_str_mv 2017-09-25
dc.date.accessioned.fl_str_mv 2019-04-23T23:51:43Z
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dc.identifier.citation.fl_str_mv SILVA, Gabriela Carinhanha. Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae). 2017. 114f. Tese (Doutorado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017.
dc.identifier.uri.fl_str_mv http://tede2.uefs.br:8080/handle/tede/741
identifier_str_mv SILVA, Gabriela Carinhanha. Elicitores na produ??o, composi??o e a??o biol?gica de metab?litos DELippia origanoides kunth (verbenaceae). 2017. 114f. Tese (Doutorado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017.
url http://tede2.uefs.br:8080/handle/tede/741
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dc.publisher.department.fl_str_mv DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
publisher.none.fl_str_mv Universidade Estadual de Feira de Santana
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