Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Rebou?as, Teresa Cristina Souza lattes
Orientador(a): Lucchese, Ang?lica Maria lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Feira de Santana
Programa de Pós-Graduação: Mestrado Acad?mico em Recursos Gen?ticos Vegetais
Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
País: Brasil
Palavras-chave em Português:
Corymbia
Compostos fen?licos
Antioxidante
Analgesia
Anti-inflamat?rio
Palavras-chave em Inglês:
Phenolic compounds
Antioxidant
Analgesia
Anti-inflammatory
Área do conhecimento CNPq:
GENETICA::GENETICA VEGETAL
Link de acesso: http://localhost:8080/tede/handle/tede/543
Resumo: Corymbia torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson (Myrtaceae) is a pollinated species, easy to adapt, high resistance and therapeutic potential. Considering that pollen has demonstrated medicinal potential, this study aimed to evaluate the chemical composition and biological activity of the pollen of this species. The material was collected at Bahia Specialty Cellulose / Copener. The chemical composition (phenolic content, total flavonoids and phytochemical screening) was evaluated from the raw ethanolic pollen extract (EEP) and the biological tests of antioxidant activity, 2,2-diphenyl-1-picryl-hydrazila (DPPH) (Rota rod), analgesic activity (tests of abdominal contortions induced by acetic acid, formalin and hot plate) and anti-inflammatory (carrageenan-induced paw edema test) were analyzed using mice. The presence of terpenes, steroids and phenolic compounds was identified and the EEP presented 116.21 ? 7.80 mgEAG / g of phenolic compounds and 35.81 ? 8.37 mgEQ / g of flavonoids. In the antioxidant activity test, pollen was able to sequester DPPH with EC50 of 60.85 ? 4.70 ?g / mL. As for the acute toxicity, no change was observed in the test used, nor was there any alteration in motor coordination in the Rota rod test. It was verified the presence of analgesic and anti-inflammatory activity of the EEP in the doses used (75, 150 and 300 mg / kg), and the dose of 75 mg / kg presented the best result. The results indicate an antioxidant, analgesic and anti-inflammatory potential for the EEP of this species, besides a possible peripheral and central activity.
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spelling Lucchese, Ang?lica Maria39684636091http://lattes.cnpq.br/584045281802431389415566515http://lattes.cnpq.br/3917750454985324Rebou?as, Teresa Cristina Souza2018-01-23T00:22:49Z2017-08-29REBOU?AS, Teresa Cristina Souza. Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae). 2017. 82 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017http://localhost:8080/tede/handle/tede/543Corymbia torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson (Myrtaceae) is a pollinated species, easy to adapt, high resistance and therapeutic potential. Considering that pollen has demonstrated medicinal potential, this study aimed to evaluate the chemical composition and biological activity of the pollen of this species. The material was collected at Bahia Specialty Cellulose / Copener. The chemical composition (phenolic content, total flavonoids and phytochemical screening) was evaluated from the raw ethanolic pollen extract (EEP) and the biological tests of antioxidant activity, 2,2-diphenyl-1-picryl-hydrazila (DPPH) (Rota rod), analgesic activity (tests of abdominal contortions induced by acetic acid, formalin and hot plate) and anti-inflammatory (carrageenan-induced paw edema test) were analyzed using mice. The presence of terpenes, steroids and phenolic compounds was identified and the EEP presented 116.21 ? 7.80 mgEAG / g of phenolic compounds and 35.81 ? 8.37 mgEQ / g of flavonoids. In the antioxidant activity test, pollen was able to sequester DPPH with EC50 of 60.85 ? 4.70 ?g / mL. As for the acute toxicity, no change was observed in the test used, nor was there any alteration in motor coordination in the Rota rod test. It was verified the presence of analgesic and anti-inflammatory activity of the EEP in the doses used (75, 150 and 300 mg / kg), and the dose of 75 mg / kg presented the best result. The results indicate an antioxidant, analgesic and anti-inflammatory potential for the EEP of this species, besides a possible peripheral and central activity.Corymbia torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson (Myrtaceae) ? uma esp?cie polin?fera, de f?cil adapta??o, grande resist?ncia e poss?veis potencialidades terap?uticas. Considerando que o p?len tem demonstrado potencial medicinal, este estudo teve como objetivo avaliar a composi??o qu?mica e atividade biol?gica do p?len dessa esp?cie. A coleta do material foi realizada na empresa Bahia Specialty Cellulose/Copener. A partir do extrato bruto etan?lico do p?len (EEP) foi avaliada a composi??o qu?mica (teor de fen?licos, flavonoides totais e triagem fitoqu?mica) e realizado testes biol?gicos de atividade antioxidante, m?todo 2,2?Difenil?1?picril?hidrazila (DPPH), utilizando camundongos analisou-se toxidade aguda, coordena??o motora (Rota rod), atividade analg?sica (testes das contor??es abdominal induzido pelo ?cido ac?tico, da formalina e da placa quente) e anti-inflamat?ria (teste do edema da pata induzido por carragenina). Foi identificada a presen?a de terpenos, esteroides e compostos fen?licos e o EEP apresentou 116,21?7,80 mgEAG/g de compostos fen?licos e 35,81?8,37 mgEQ/g de flavonoides. No teste de atividade antioxidante o p?len foi capaz de sequestrar o DPPH com CE50 de 60,85?4,70 ?g/mL. Quanto ? toxidade aguda n?o foi observada nenhuma altera??o no teste utilizado, bem como n?o foi verificada altera??es da coordena??o motora no teste do Rota rod. Foi verificada a presen?a de atividade analg?sica e anti-inflamat?ria do EEP nas doses utilizadas (75, 150 e 300 mg/kg), sendo que a dose de 75 mg/kg apresentou o melhor resultado. Os resultados obtidos apontam um potencial antioxidante, analg?sico e anti-inflamat?rio para o EEP dessa esp?cie, al?m de uma poss?vel atividade perif?rica e central.Submitted by Jadson Francisco de Jesus SILVA (jadson@uefs.br) on 2018-01-23T00:22:49Z No. of bitstreams: 1 Disserta??o vers?o biblioteca FINAL II Teresa_versao WORD (1) (1).pdf: 2399848 bytes, checksum: a72bd616ac1f702167982548cd4418bb (MD5)Made available in DSpace on 2018-01-23T00:22:49Z (GMT). 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dc.title.por.fl_str_mv Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
title Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
spellingShingle Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
Rebou?as, Teresa Cristina Souza
Corymbia
Compostos fen?licos
Antioxidante
Analgesia
Anti-inflamat?rio
Phenolic compounds
Antioxidant
Analgesia
Anti-inflammatory
GENETICA::GENETICA VEGETAL
title_short Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
title_full Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
title_fullStr Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
title_full_unstemmed Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
title_sort Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae)
author Rebou?as, Teresa Cristina Souza
author_facet Rebou?as, Teresa Cristina Souza
author_role author
dc.contributor.advisor1.fl_str_mv Lucchese, Ang?lica Maria
dc.contributor.advisor1ID.fl_str_mv 39684636091
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5840452818024313
dc.contributor.authorID.fl_str_mv 89415566515
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3917750454985324
dc.contributor.author.fl_str_mv Rebou?as, Teresa Cristina Souza
contributor_str_mv Lucchese, Ang?lica Maria
dc.subject.por.fl_str_mv Corymbia
Compostos fen?licos
Antioxidante
Analgesia
Anti-inflamat?rio
topic Corymbia
Compostos fen?licos
Antioxidante
Analgesia
Anti-inflamat?rio
Phenolic compounds
Antioxidant
Analgesia
Anti-inflammatory
GENETICA::GENETICA VEGETAL
dc.subject.eng.fl_str_mv Phenolic compounds
Antioxidant
Analgesia
Anti-inflammatory
dc.subject.cnpq.fl_str_mv GENETICA::GENETICA VEGETAL
description Corymbia torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson (Myrtaceae) is a pollinated species, easy to adapt, high resistance and therapeutic potential. Considering that pollen has demonstrated medicinal potential, this study aimed to evaluate the chemical composition and biological activity of the pollen of this species. The material was collected at Bahia Specialty Cellulose / Copener. The chemical composition (phenolic content, total flavonoids and phytochemical screening) was evaluated from the raw ethanolic pollen extract (EEP) and the biological tests of antioxidant activity, 2,2-diphenyl-1-picryl-hydrazila (DPPH) (Rota rod), analgesic activity (tests of abdominal contortions induced by acetic acid, formalin and hot plate) and anti-inflammatory (carrageenan-induced paw edema test) were analyzed using mice. The presence of terpenes, steroids and phenolic compounds was identified and the EEP presented 116.21 ? 7.80 mgEAG / g of phenolic compounds and 35.81 ? 8.37 mgEQ / g of flavonoids. In the antioxidant activity test, pollen was able to sequester DPPH with EC50 of 60.85 ? 4.70 ?g / mL. As for the acute toxicity, no change was observed in the test used, nor was there any alteration in motor coordination in the Rota rod test. It was verified the presence of analgesic and anti-inflammatory activity of the EEP in the doses used (75, 150 and 300 mg / kg), and the dose of 75 mg / kg presented the best result. The results indicate an antioxidant, analgesic and anti-inflammatory potential for the EEP of this species, besides a possible peripheral and central activity.
publishDate 2017
dc.date.issued.fl_str_mv 2017-08-29
dc.date.accessioned.fl_str_mv 2018-01-23T00:22:49Z
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dc.identifier.citation.fl_str_mv REBOU?AS, Teresa Cristina Souza. Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae). 2017. 82 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017
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identifier_str_mv REBOU?AS, Teresa Cristina Souza. Composi??o qu?mica e atividade biol?gica do extrato bruto etan?lico do p?len de Corymbia Torelliana (F. Muell.) K.D. Hill & L.A.S. Johnson. (Myrtaceae). 2017. 82 f. Disserta??o (Mestrado Acad?mico em Recursos Gen?ticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2017
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