An?lise explorat?ria e preditiva de linhagens de caup? baseada nas respostas oxidativas ? presen?a de alum?nio

• Ano de defesa: 2023
• Autor(a) principal: Oliveira, Uasley Caldas de
• Orientador(a): Ribeiro, Marilza Neves do Nascimento
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual de Feira de Santana
• Programa de Pós-Graduação: Doutorado Acad?mico em Recursos Gen?ticos Vegetais
• Departamento: DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
• País: Brasil
• Palavras-chave em Português: Feij?o-caup?; Vigna unguiculata (L.) Walp.; Alum?nio; Concentra??o; Modelagem preditiva; Estresse abi?tico; Catalase; Esp?cies reativas de oxig?nio; Aprendizado de m?quina
• Palavras-chave em Inglês: Vigna unguiculata (L.) Walp.; Abiotic stress; Machine learning; Reactive oxygen species; Random Forest; Aluminum; Concentration
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA; GENETICA::GENETICA VEGETAL; FITOTECNIA::PRODUCAO E BENEFICIAMENTO DE SEMENTES; CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: http://tede2.uefs.br:8080/handle/tede/1651

Aluminum toxicity is a significant limiting factor in cowpea (Vigna unguiculata L.) cultivation, a legume grown in tropical and subtropical regions, highly valued as a food source, particularly for its nutritional and socio-economic importance to small-scale farmers in the North and Northeast regions of Brazil. The objective of this study was to assess cowpea lineages for aluminum ion tolerance based on the specific activity of antioxidant enzymes using predictive modeling. The experiment was conducted at the State University of Feira de Santana (UEFS), in the Seed Germination Laboratory (LAGER), and in a greenhouse. Evaluation of protein content and the specific activity of the enzymes ascorbate peroxidase (APx), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) was performed on plants exposed to different aluminum concentrations. Various forms of aluminum tolerance classification were used to analyze the results, including mean tests using Scott-Knott with a probability level of p<0.05. Additionally, predictive modeling was employed, incorporating data trees, where predictive models like Random Forest, Tree, Neural Network, and kNN were tested. Evans blue dye was utilized as a visual indicator of aluminum toxicity, and its quantification was done through spectrophotometry. Significant genetic variability was observed among cowpea lineages concerning aluminum tolerance. The enzyme activity data from plants exposed to aluminum ions enabled the determination that the Random Forest and Neural Network models for images with Evans Blue dye displayed the best predictive capability for both data sets. Using the visual method with Evans blue dye, lineage O demonstrated the highest tolerance, while lineages I, J, F, G, and M were the most sensitive to aluminum, as determined by root apex coloration. Regarding Evans blue quantification, lineages D and C were the most tolerant, and the most sensitive lineages were G and A.