Expressão de genes envolvidos com o desenvolvimento do botão floral do algodoeiro (Gossypium hirsutum H.) por meio de RT-PCR e RT-qPCR

• Ano de defesa: 2012
• Autor(a) principal: Batista, Vandré Guevara Lyra
• Orientador(a): Lima, Liziane Maria de
• Banca de defesa: Campos, Magnólia de Araújo , Fernandes, Pedro Dantas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual da Paraíba
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Agrárias - PPGCA
• Departamento: Ciências Agrárias
• País: BR
• Palavras-chave em Português: Genética vegetal; Algodoeiro; GenBank
• Área do conhecimento CNPq: CNPQ
• Link de acesso: https://repositorio.uepb.edu.br/handle/123456789/75355

The use of public database, generated from genome projects, such as NCBI, CottonDB, SUCEST FORESTs, among others, is of great importance for molecular studies, especially those related to gene expression, since, in possession of sequences deposited, it is possible to isolate new genes or prospect and know their role in various ontogenetic stages. In plants, a stage of great demand for knowledge is related to reproduction. Currently, several studies with Arabidopsis thaliana involving identification and characterization of genes associated with reproductive organs, especially the bud phenology, has enabled major advances in our understanding of functions and can correlate them with other species. For plant species possessing large commodities such as cotton, these results are relevant, considering that several studies developed with this aim culture studies that enhance the expression quantitative trait dependent physiology of reproduction. In this study, we investigated the temporal and spatial expression of genes that are expressed in cotton bud by semiquantitative RT-PCR and qPCR. We selected four genes with a history of expression in floral buds (cottonbud7, cottonbud8, and cottonbud9 cottonbud10) and used for primer design. Tissue samples of flower buds (2-8 mm, 10-12 mm and 14-20 mm), leaves, stems and roots were collected and used for total RNA extraction and cDNA synthesis. The results of gene expression analyzes showed expression of selected genes in all tissues investigated, though genes cottonbud7 cottonbud10 and showed a stable expression in all phases investigated bud. It was observed further that the cottonbud10 showed a slightly higher expression in floral bud, when compared to other genes. The analysis of gene expression via cottonbud10 qPCR corroborate the results obtained with the RT-PCR. The results obtained in this study provide information about genes to promising research projects involving the improvement program for cotton.