Avaliação da mortalidade do bicudo-do-algodoeiro em algodoeiros transgênicos CRY10AA e metodologia para sua determinação

• Ano de defesa: 2025
• Autor(a) principal: Lima, Adrielly Karoliny de
• Orientador(a): Silva, Carlos Alberto Domingues da
• Banca de defesa: Lima, Liziane Maria , Malaquias, José Bruno
• Tipo de documento: Tese
• Tipo de acesso: Acesso embargado
• Idioma: por
• Instituição de defesa: Universidade Estadual da Paraíba
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Agrárias - PPGCA
• Departamento: Centro de Ciências Agrárias e Ambientais – CCAA; Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP
• País: BR
• Palavras-chave em Português: Anthonomus grandis; Gossypium hirsutum; toxina Bt; praga de inseto; Bt toxin
• Palavras-chave em Inglês: Anthonomus grandis; Gossypium hirsutum; insect pest
• Área do conhecimento CNPq: FITOSSANIDADE
• Link de acesso: https://repositorio.uepb.edu.br/handle/123456789/75493

The use of transgenic cotton plants resistant to the boll weevil, Anthonomus grandis (Coleoptera: Curculionidae) can reduce the use of chemical pesticides in the main cotton-producing regions of Brazil. The efficacy of BRS 372 cotton progenies with the Cry10Aa gene to the boll weevil was evaluated and a methodology to determine the specific causes of mortality induced by the Bt protein was proposed. The experimental design was a randomized block design with six treatments, the non-transgenic isoline (control: BRS 372) and five transgenic cotton progenies with the pCotton3/AGRA8 construction: F54-14, F57-04, F57-07, F57-16, and F57-24, with four replications. Mated A. grandis females (290) were released after 40, 61 and 68 days, of its emergence, on plants of similar phenological stages with cotton squares from the fortieth day onwards. The formation of bolls from oviposited cotton squares and egg infertility, proliferation of internal cells or the formation of a gelatinous substance evidenced that the Bt protein did not affect the immature stages of the boll weevil. The absence of larvae (alive or dead) of the boll weevil inside the aborted cotton squares (suggesting death before development) without traces and partially damaged anthers or remains of dead insect with natural coloration, reduced size, and preserved cuticle (without desiccation) characterize the action of the entomotoxic protein Cry10Aa in GM plants. The proposed methodology, based on a descriptive flowchart with photographs, was effective to determining the causes of weevil mortality in floral buds of transgenic cotton plants.