Desenvolvimento de filmes de quitosana incorporados com membrana celular do ovo para aplicações biomédicas

• Ano de defesa: 2018
• Autor(a) principal: Santos, Kleilton Oliveira
• Orientador(a): Oliveira, Rodrigo José de
• Banca de defesa: Buriti, Josué da Silva , Alves, Mary Cristina Ferreira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual da Paraíba
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química - PPGQ
• Departamento: Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP
• País: BR
• Palavras-chave em Português: Curativos; Membrana celular do ovo; Quitosana; Biomateriais
• Palavras-chave em Inglês: Chitosan; Eggshell membrane; Dressings; Biomaterials
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA
• Link de acesso: https://repositorio.uepb.edu.br/handle/123456789/73607

Each year researchers are seeking to develop or improve materials that minimize health problems and promote or assist tissue regeneration. Biomaterials allow to meet the needs of the organism in order to assist in the reconstructive processes and consequently improve the quality of life of the population. In this context, the use of biopolymers such as chitosan has been studied, since it is a natural polysaccharide that presents a great potential as biomaterial, since it presents antimicrobial activity, atoxicity, biocompatibility, biodegradability, cicatrizant power, besides forming viscous solutions and movies. Their properties may be suitable for use in dressings with the addition of the egg cell membrane. The cell membrane of the chicken egg is composed of fibrous proteins such as collagen, hyaluronic acid and a host of other nutrients, naturally occurring substances and play an essential role in maintaining connective tissue, reducing pain and stiffness in the joints. Thus, the objective of this work is to develop films composed of chitosan and cell membrane of the chicken egg as dressings for use in the treatment of burns. The chitosan used in the development of this work was provided by the Laboratory of Evaluation and Development of Northeastern Biomaterials (CERTBIO). Cell membranes from the chicken eggs were manually extracted and dehydrated in an oven at 60 ° C for 2 days. Subsequently, the membranes were ground and passed through a 325 mesh sieve, until a fine powder was obtained. The films were obtained by the solvent evaporation method, where 1% (m/v) chitosan solutions were prepared in 0.1 mol/L lactic acid and chitosan solutions incorporated with the egg cell membrane powder (MCO ) in the proportions of 10, 20 and 30% (m / m). The solutions were mechanically agitated at 600 rpm for 24 hours, then poured into acrylic Petri dishes and oven dried at 40 ° C for 24 hours. After the films were obtained, they were neutralized in a 2% ammonium hydroxide atmosphere for 72 h. The materials and samples obtained were characterized by Thermogravimetry (TGA), Differential Exploration Calorimetry (DSC), Scanning Electron Microscopy (SEM), X-ray Dispersive Energy (EDS) Spectroscopy, X-ray Diffraction (XRD) and Spectroscopy Infrared with Fourier Transform (FTIR). In addition to these tests, the degree of deacetylation and the viscosity molar mass were performed for the chitosan. For the cell membrane powder, moisture, ash, protein, lipid and carbohydrate tests were performed. For the films developed, measurements of thickness, wettability, mechanical properties, water vapor permeability and biological evaluation were performed. The results showed that the chitosan has a semicrystalline profile, degradation temperature above 250 ° C, degree of deacetylation of 85% and medium molecular weight. The powder extracted from the cell membrane of the chicken egg presented characteristics of a semi-crystalline, fibrillar, protein rich material, with initial collagen denaturation temperature around 67 ºC and powder degradation temperature above 220 ºC. As for the thermal, physicochemical and mechanical stability of the films, the addition of charge in the chitosan polymer matrix caused inst ability in the material. They had a thickness of 0.05 mm, hydrophilic character, permeability to water vapor. Regarding the biological evaluation, the materials presented adequate viability. The samples with 10% of load had a better interaction with the chitosan matrix, evidencing a better stability of the films. Thus, it was possible to obtain films added with OLS for application as dressings in the treatment of burns.