Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína
Ano de defesa: | 2023 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso embargado |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual da Paraíba
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF
|
Departamento: |
Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.bc.uepb.edu.br/jspui/handle/tede/4518 |
Resumo: | Papain (PAP) is an enzyme that has several pharmacological activities such as healing, antimicrobial and anti-inflammatory properties. However, it has low stability, which is a problem for the development of therapeutic formulations. In view of this, a strategy to overcome this adversity is to perform immobilization through the PAP adsorption technique on substrates, for example, laponite® (LAP). Thus, the objective of this work was to develop and characterize a nanostructured hybrid system (SHN) composed of LAP and PAP. For this, the experimental conditions of adsorption of this enzyme in LAP were evaluated, considering the effect of pH, initial concentration of PAP and contact time. Equilibrium and kinetic studies were carried out within the range of pH 5.0 to 7.0, with reaction times from 0 to 2880 min and with concentrations from 4.500 to 25.500 mg/L in sodium phosphate buffer, under stirring at 200 rpm. Langmuir, Freundlich and Temkin isothermal models were used, as well as pseudo-first order, pseudo-second order and Elovich kinetics. The SHN were characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric and derivative analysis (TG and DTG), differential thermal analysis (DTA) and X-ray diffraction (XRD). The results showed that the interaction between PAP-LAP is influenced by pH, with maximum adsorption at pH 7, the isotherm study showed that the equilibrium conditions of PAP-LAP adsorption obey the Freundlich model. Adsorption kinetics showed a fast phase up to 60 min and a slow one from 120 to 2880 min, fitting the pseudo-second order model. The maximum amount of PAP adsorbed on the LAP was 1243.6 mg/g, obtained at a concentration of 15.500 mg/L, at pH 7, in a time of 2880 min. The intercalation of PAP in the LAP was verified by the FTIR and XRD patterns, since an increase in the interlamellar distance from 1.252 nm (LAP) to 1.315 nm (SHN) was seen, with SHN spectra showing smaller vibrations referring to the PAP in the LAP, when compared to the isolated spectra of the drug. The mass loss profile in relation to time, demonstrated from the results obtained from TG/DTG/DTA, highlighted that the greatest losses occurred at pH 7 and at the highest concentration of drug, which was the work standard chosen. Considering the results obtained, it can be concluded that the process of adsorption of PAP in LAP promotes the feasibility of obtaining a SHN, with the ability to overcome the low enzymatic stability. It was proved that the increase in the concentration of PAP and the pH of the medium caused an increase in adsorption, at pH 7 there is formation of a heterogeneous surface and the adsorption occurs in multilayer, which is related to the intercalation capacity of PAP in the LAP lamellae. It is possible to point out that the longer the contact time between the adsorbate and the adsorbent, the greater the adsorption capacity due to the exfoliation of the lamellae of this clay mineral, this process represents a viable alternative to remedy the industrial need for PAP immobilization. |
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Damasceno, Bolívar Ponciano Goulart de Limahttp://lattes.cnpq.br/6407334157973308Silva, Dayanne Tomaz Casimiro dahttp://lattes.cnpq.br/6515613697746626Lima, Ádley Antonini Neves deSilva, Paulo César Dantas daRocha, Wilma Raianny Vieira da12202338462http://lattes.cnpq.br/7090506973472469Medeiros, Kaline de Araújo2023-04-05T14:34:02Z2999-12-312023-01-10MEDEIROS, Kaline de Araújo. Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína. 2023. 67f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2023.http://tede.bc.uepb.edu.br/jspui/handle/tede/4518Papain (PAP) is an enzyme that has several pharmacological activities such as healing, antimicrobial and anti-inflammatory properties. However, it has low stability, which is a problem for the development of therapeutic formulations. In view of this, a strategy to overcome this adversity is to perform immobilization through the PAP adsorption technique on substrates, for example, laponite® (LAP). Thus, the objective of this work was to develop and characterize a nanostructured hybrid system (SHN) composed of LAP and PAP. For this, the experimental conditions of adsorption of this enzyme in LAP were evaluated, considering the effect of pH, initial concentration of PAP and contact time. Equilibrium and kinetic studies were carried out within the range of pH 5.0 to 7.0, with reaction times from 0 to 2880 min and with concentrations from 4.500 to 25.500 mg/L in sodium phosphate buffer, under stirring at 200 rpm. Langmuir, Freundlich and Temkin isothermal models were used, as well as pseudo-first order, pseudo-second order and Elovich kinetics. The SHN were characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric and derivative analysis (TG and DTG), differential thermal analysis (DTA) and X-ray diffraction (XRD). The results showed that the interaction between PAP-LAP is influenced by pH, with maximum adsorption at pH 7, the isotherm study showed that the equilibrium conditions of PAP-LAP adsorption obey the Freundlich model. Adsorption kinetics showed a fast phase up to 60 min and a slow one from 120 to 2880 min, fitting the pseudo-second order model. The maximum amount of PAP adsorbed on the LAP was 1243.6 mg/g, obtained at a concentration of 15.500 mg/L, at pH 7, in a time of 2880 min. The intercalation of PAP in the LAP was verified by the FTIR and XRD patterns, since an increase in the interlamellar distance from 1.252 nm (LAP) to 1.315 nm (SHN) was seen, with SHN spectra showing smaller vibrations referring to the PAP in the LAP, when compared to the isolated spectra of the drug. The mass loss profile in relation to time, demonstrated from the results obtained from TG/DTG/DTA, highlighted that the greatest losses occurred at pH 7 and at the highest concentration of drug, which was the work standard chosen. Considering the results obtained, it can be concluded that the process of adsorption of PAP in LAP promotes the feasibility of obtaining a SHN, with the ability to overcome the low enzymatic stability. It was proved that the increase in the concentration of PAP and the pH of the medium caused an increase in adsorption, at pH 7 there is formation of a heterogeneous surface and the adsorption occurs in multilayer, which is related to the intercalation capacity of PAP in the LAP lamellae. It is possible to point out that the longer the contact time between the adsorbate and the adsorbent, the greater the adsorption capacity due to the exfoliation of the lamellae of this clay mineral, this process represents a viable alternative to remedy the industrial need for PAP immobilization.A papaína (PAP) é uma enzima que possui várias atividades farmacológicas como propriedade cicatrizante, antimicrobiana e anti-inflamatória. Porém, apresenta baixa estabilidade, o que é um problema para o desenvolvimento de formulações terapêuticas. Diante disso, uma estratégia para contornar essa adversidade é a execução da imobilização através da técnica de adsorção da PAP em substratos, por exemplo, a laponita® (LAP). Assim, o objetivo desse trabalho foi desenvolver e caracterizar um sistema híbrido nanoestruturado (SHN) composto de LAP e PAP. Para isso foram avaliadas as condições experimentais de adsorção dessa enzima na LAP, considerando o efeito do pH, da concentração inicial da PAP e do tempo de contato. Os estudos de equilíbrio e de cinética foram realizados dentro da faixa de pH 5,0 a 7,0, com tempos de reação de 0 a 2880 min e com concentrações de 4.500 a 25.500 mg/L em tampão fosfato de sódio, sob agitação a 200 rpm. Foram utilizados os modelos isotérmicos de Langmuir, Freundlich e Temkin, e os cinéticos de pseudo-primeira ordem, pseudo-segunda ordem e Elovich. Os SHN foram caracterizados pelas técnicas de espectroscopia de infravermelho por transformada de Fourier (FTIR), análise termogravimétrica e derivada (TG e DTG), análise térmica diferencial (DTA) e difração de raio-X (DRX). Os resultados apontaram que a interação entre PAP-LAP é influenciada pelo pH, detendo de máxima adsorção em pH 7, o estudo de isoterma evidenciou que as condições de equilíbrio da adsorção de PAP-LAP obedecem ao modelo de Freundlich. A cinética de adsorção apresentou uma fase rápida até 60 min e uma lenta de 120 a 2880 min, ajustando-se ao modelo de pseudo-segunda ordem. A quantidade máxima de PAP adsorvida na LAP foi de 1243,6 mg/g, obtida na concentração de 15.500 mg/L, em pH 7, no tempo de 2880 min. A intercalação da PAP na LAP foi constatada pelos padrões de FTIR e DRX, uma vez que foi visto uma elevação na distância interlamelar de 1,252 nm (LAP) para 1,315 nm (SHN), com espectros dos SHN mostrando vibrações menores referentes à PAP na LAP, quando confrontado aos espectros isolados do fármaco. O perfil de perda de massa em relação ao tempo, demonstrado a partir dos resultados obtidos do TG/DTG/DTA, salientaram que as maiores perdas ocorreram no pH 7 e na maior concentração de fármaco, que foi o padrão de trabalho escolhido. Considerando os resultados obtidos, pode-se concluir que o processo de adsorção de PAP em LAP promove viabilidade de obtenção de um SHN, com capacidade de contornar a baixa estabilidade enzimática. Foi provado que a elevação da concentração da PAP e do pH do meio provocaram aumento da adsorção, em pH 7 há formação de uma superfície heterogênea e a adsorção ocorre em multicamada, o que está relacionado a capacidade de intercalação da PAP nas lamelas de LAP. É possível ressaltar que quanto maior o tempo de contato entre o adsorvato e o adsorvente, maior a capacidade de adsorção devido a esfoliação das lamelas deste argilomineral, esse processo representa uma alternativa viável para sanar a necessidade industrial de imobilização da PAP.Submitted by kalinearaujo9197@hotmail.com (kalinearaujo9197@hotmail.com) on 2023-02-25T23:54:50Z No. of bitstreams: 2 DS - Kaline de Araújo Medeiros.pdf: 1860385 bytes, checksum: bc8068ea9d72a5f460791ada82b2514d (MD5) Termo de Depósito BDTD - Kaline de Araújo Medeiros .pdf: 76173 bytes, checksum: d25421002aedb89a0ecdf90ac9d568d3 (MD5)Approved for entry into archive by Jean Medeiros (jeanletras@uepb.edu.br) on 2023-02-27T17:30:25Z (GMT) No. of bitstreams: 2 DS - Kaline de Araújo Medeiros.pdf: 1860385 bytes, checksum: bc8068ea9d72a5f460791ada82b2514d (MD5) Termo de Depósito BDTD - Kaline de Araújo Medeiros .pdf: 76173 bytes, checksum: d25421002aedb89a0ecdf90ac9d568d3 (MD5)Made available in DSpace on 2023-04-05T14:34:02Z (GMT). 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dc.title.por.fl_str_mv |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
title |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
spellingShingle |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína Medeiros, Kaline de Araújo Papaína Argilominerais Preparações farmacêuticas Pharmaceutical preparations Clay minerals Papain CIENCIAS DA SAUDE::FARMACIA FARMACIA::FARMACOTECNIA FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
title_short |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
title_full |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
title_fullStr |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
title_full_unstemmed |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
title_sort |
Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína |
author |
Medeiros, Kaline de Araújo |
author_facet |
Medeiros, Kaline de Araújo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Damasceno, Bolívar Ponciano Goulart de Lima |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6407334157973308 |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Dayanne Tomaz Casimiro da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6515613697746626 |
dc.contributor.referee1.fl_str_mv |
Lima, Ádley Antonini Neves de |
dc.contributor.referee2.fl_str_mv |
Silva, Paulo César Dantas da |
dc.contributor.referee3.fl_str_mv |
Rocha, Wilma Raianny Vieira da |
dc.contributor.authorID.fl_str_mv |
12202338462 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7090506973472469 |
dc.contributor.author.fl_str_mv |
Medeiros, Kaline de Araújo |
contributor_str_mv |
Damasceno, Bolívar Ponciano Goulart de Lima Silva, Dayanne Tomaz Casimiro da Lima, Ádley Antonini Neves de Silva, Paulo César Dantas da Rocha, Wilma Raianny Vieira da |
dc.subject.por.fl_str_mv |
Papaína Argilominerais Preparações farmacêuticas |
topic |
Papaína Argilominerais Preparações farmacêuticas Pharmaceutical preparations Clay minerals Papain CIENCIAS DA SAUDE::FARMACIA FARMACIA::FARMACOTECNIA FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
dc.subject.eng.fl_str_mv |
Pharmaceutical preparations Clay minerals Papain |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA FARMACIA::FARMACOTECNIA FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
description |
Papain (PAP) is an enzyme that has several pharmacological activities such as healing, antimicrobial and anti-inflammatory properties. However, it has low stability, which is a problem for the development of therapeutic formulations. In view of this, a strategy to overcome this adversity is to perform immobilization through the PAP adsorption technique on substrates, for example, laponite® (LAP). Thus, the objective of this work was to develop and characterize a nanostructured hybrid system (SHN) composed of LAP and PAP. For this, the experimental conditions of adsorption of this enzyme in LAP were evaluated, considering the effect of pH, initial concentration of PAP and contact time. Equilibrium and kinetic studies were carried out within the range of pH 5.0 to 7.0, with reaction times from 0 to 2880 min and with concentrations from 4.500 to 25.500 mg/L in sodium phosphate buffer, under stirring at 200 rpm. Langmuir, Freundlich and Temkin isothermal models were used, as well as pseudo-first order, pseudo-second order and Elovich kinetics. The SHN were characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric and derivative analysis (TG and DTG), differential thermal analysis (DTA) and X-ray diffraction (XRD). The results showed that the interaction between PAP-LAP is influenced by pH, with maximum adsorption at pH 7, the isotherm study showed that the equilibrium conditions of PAP-LAP adsorption obey the Freundlich model. Adsorption kinetics showed a fast phase up to 60 min and a slow one from 120 to 2880 min, fitting the pseudo-second order model. The maximum amount of PAP adsorbed on the LAP was 1243.6 mg/g, obtained at a concentration of 15.500 mg/L, at pH 7, in a time of 2880 min. The intercalation of PAP in the LAP was verified by the FTIR and XRD patterns, since an increase in the interlamellar distance from 1.252 nm (LAP) to 1.315 nm (SHN) was seen, with SHN spectra showing smaller vibrations referring to the PAP in the LAP, when compared to the isolated spectra of the drug. The mass loss profile in relation to time, demonstrated from the results obtained from TG/DTG/DTA, highlighted that the greatest losses occurred at pH 7 and at the highest concentration of drug, which was the work standard chosen. Considering the results obtained, it can be concluded that the process of adsorption of PAP in LAP promotes the feasibility of obtaining a SHN, with the ability to overcome the low enzymatic stability. It was proved that the increase in the concentration of PAP and the pH of the medium caused an increase in adsorption, at pH 7 there is formation of a heterogeneous surface and the adsorption occurs in multilayer, which is related to the intercalation capacity of PAP in the LAP lamellae. It is possible to point out that the longer the contact time between the adsorbate and the adsorbent, the greater the adsorption capacity due to the exfoliation of the lamellae of this clay mineral, this process represents a viable alternative to remedy the industrial need for PAP immobilization. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-04-05T14:34:02Z |
dc.date.issued.fl_str_mv |
2023-01-10 |
dc.date.available.fl_str_mv |
2999-12-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MEDEIROS, Kaline de Araújo. Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína. 2023. 67f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2023. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4518 |
identifier_str_mv |
MEDEIROS, Kaline de Araújo. Desenvolvimento de um sistema híbrido nanoestruturado de Laponita® e Papaína. 2023. 67f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2023. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4518 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
8157968555251876874 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
dc.relation.department.fl_str_mv |
524871450381110278 |
dc.relation.cnpq.fl_str_mv |
6997636413449754996 -1498234366838932019 6216025074656932336 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF |
dc.publisher.initials.fl_str_mv |
UEPB |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEPB instname:Universidade Estadual da Paraíba (UEPB) instacron:UEPB |
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Universidade Estadual da Paraíba (UEPB) |
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UEPB |
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UEPB |
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Biblioteca Digital de Teses e Dissertações da UEPB |
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Biblioteca Digital de Teses e Dissertações da UEPB |
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