Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1

• Ano de defesa: 2009
• Autor(a) principal: Pandini, Juliana Almeida
• Orientador(a): Gomes, Marilia de Brito
• Banca de defesa: Silva, Adriana Ribeiro , Avesani, Carla Maria , Oliveira, Cecilia Lacroix de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Fisiopatologia Clínica e Experimental
• Departamento: Centro Biomédico::Faculdade de Ciências Médicas
• País: BR
• Palavras-chave em Português: Diabetes tipo 1; LDL oxidado; Suscetibilidade
• Palavras-chave em Inglês: Type 1 diabetes; Oxidized LDL; Susceptibility
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::NUTRICAO
• Link de acesso: http://www.bdtd.uerj.br/handle/1/12798

With the intensive glycemic control and longer survival of type 1 diabetes mellitus (T1DM) patients, cardiovascular disease has been the main cause of mortality. Oxidized LDL is considered a critical factor in the atherosclerotic process and the aim of our study was evaluate the absorbance of different wavelengths of oxidized phospholipids and oxidation rate of LDL particles in vitro in T1DM. We studied 54 patients aged 29.1 ± 12.11 years and with a disease duration of 14.6 ± 8.43 years and 28 non-diabetic volunteers. The two groups had carried where they were measured lipid profile, glucose, uric acid, blood pressure, urinary albumin excretion and spectrophotometry analysis of the oxidation of LDL cholesterol before and 1, 3, 6 and 24 hours after addition of substance oxidant copper sulfate. The coefficients of oxidation (Cox) of LDL from both groups had similar reading at all times with a significant difference between the baseline and after 1 hour (p <0 .001) and between the time after 3 and 6 hours after addition of sulfate copper (p <0 .001) for both groups. In the multiple regression model, the result was the Cox model = 8.246 to 13.93 to 10.21 + -2.71 b c (R2 = 0.835), here "a", "b" and "c" represent the absorbance (205 nm , 232 nm and 280 nm, respectively). We conclude that in our sample the wavelengths 232 nm and 280 nm related to the reading of conjugated diene and protein faction of LDL, respectively, were more important in determining the oxidation rate of the particle.