Infiltração microbiana por Enterococcus faecalis em canais retrobturados com MTA, iRoot SP e Endo CPM Sealer

• Ano de defesa: 2011
• Autor(a) principal: Nascimento, Viviane Dias Martini Alves
• Orientador(a): Fidel, Sandra Rivera
• Banca de defesa: Fidel, Rivail Antonio Sergio , Moreira, Luciano Artioli , Cecilia, Mauricio Santa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
• Departamento: Centro Biomédico::Faculdade de Odontologia
• País: BR
• Palavras-chave em Português: Obturação retrógrada; Infiltração dentária; Enterococcus faecalis; Obturação (Odontologia); Enterococcus faecalis - Cultura e meios de cultura
• Palavras-chave em Inglês: Retrograde obturation; Dental leakage; Enterococcus faecalis
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::ENDODONTIA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/14136

The objective of this study consisted in the analysis of apical leakage in retrofilled teeth by three materials: MTA, iROOT SP and Endo CPM Sealer. 51 extracted human central incisors were instrumented manually with K-type files by Crown-down technique, filled by lateral compactation and apicectomyzed to 3mm below the apex. Then, teeth were retrofilling with the three proposed materials. The samples were divided, randomly, in three groups: GI - MTA, GII - iROOT SP and GIII - Endo CPM Sealer, each group with 15 samples. Teeth were inserted into eppendorf tubes, and the remaining root were sealed using two layers of cyanoacrylate, epoxy, and another layer of nail varnish. In each eppendorf tube were added sterile TSB broth and a suspension of Enterococcos faecalis. The body proof was adapted to a glass bottle with culture medium Enterococcosel. The bacterial leakage was checked by turbidity of the culture medium. After analysis within 60 days the bacterial leakage was recorded: the Group I showed infiltration in 43.75% of body proof, the Group II in 31.25% and Group III 25%. Positive and negative control groups for bacterial growth were performed (n = 3, each). We can conclude that the tested cements behave similarly to bacterial infiltration front during the period tested.