Micropropagação e avaliação da potencialidade genotóxica de Petiveria alliacea L.

• Ano de defesa: 2013
• Autor(a) principal: Soares, Bianka de Oliveira
• Orientador(a): Araujo, Rachel Fatima Gagliardi
• Banca de defesa: Duarte, Aline Castellar , Silva, Claudia Ribeiro da , Dantas, Flávio José da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Vegetal
• Departamento: Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes
• País: BR
• Palavras-chave em Português: Guiné; Phytolaccacea; Dibenzil dissulfeto; Cromatografia gasosa; Potencial antigenotóxico; Antioxidante; Petiveria alliacea; Ervas - Uso terapêutico
• Palavras-chave em Inglês: Guinea; Phytolaccacea; Dibenzyl disulfide; Gas chromatography; Antigenotoxic potential; Antioxidant
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::BOTANICA APLICADA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/7939

Petiveria alliacea L. popularly known in Brazil as Guiné , Amansa-senhor or Tipi belongs to the Phytolaccaceae family. Native from the Amazon region, it has been cultivated in many tropical areas for medicinal or ritualistic purposes. The aim of this study was: i) the development and multiplication of plants of P. alliacea L. through tissue culture methods as well as monitoring phytochemical cultures; ii) comparative assessment of genotoxic and antigenotoxic potentialities between field collected and in vitro grown plants. Specimens from different populations occurring in the state of Rio de Janeiro were used as matrices for the culture. It was established a protocol for propagation in MS medium supplemented with different BAP + ANA concentrations and combinations whose best result (BAP 4,4 μM + ANA 0,54 μM) provided 8 plants per explant in average. The phytochemical analysis was based on chromatographic methods of different extracts both of field and in vitro plant populations resulting in different substances identified through the Thin-layer chromatography analyzing samples. The extracts were also assayed by cromatografia gasosa acoplada á espectrometria de massas having identified various substances, including the dibenzyl disulfide, a degradation tiosulfinato s product with important function in plant defense and increasing use by the pharmaceutical industry. Field and in vitro plant aqueous extracts were evaluated for genotoxic and antigenotoxic potentialities using PUC 9.1 plasmid DNA. These results showed changes in the DNA structural conformation indicating the occurrence of single and double strand breaks induced by the extracts in a dose-response way. Finally, data also pointed to a protective effect of aqueous extract against stannous chloride oxidative damage, characterizing an antigenotoxic potentiality of aqueous extract.