Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae)
Ano de defesa: | 2010 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Alagoas
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Agronomia
|
Departamento: |
Não Informado pela instituição
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufal.br/handle/riufal/249 |
Resumo: | The genetically modified cotton to produce toxins from the bacterium Bacillus thuringiensis (Bt) has the objective of offering plant resistance against lepidopteran larvae. The cotton crop cultivated in the Semiarid region can be subjected to the condition of water stress. Thus, this work investigated the expression of the Cry1Ac toxin in two varieties of Bt-cotton Acala 90B and NuOpal cultivated under the conditions of irrigated and water stress. In addition, the oviposition and feeding preference of adults and larvae of the cotton leafworm, Alabama Argillacea (Hübner), caged on these plants were studied. Cotton plants were cultivated under controlled conditions of irrigation to obtain water stress treatment in microparcels inside an open-sided greenhouse. Higher levels of Cry1Ac expression was found on top leaves of Bt-cotton for both varieties under water stress condition, and similar levels between plants with and without water stress in the other plant parts such as bracts, flower petals, and boll. Adults of A. argillacea did not exhibited preference for oviposition between Bt and non-Bt cotton plants. Likewise, 3- and 10-d-old A. argillacea larvae did not show food preference under a choice test comparing leaves of non-Bt and Bt-cotton plants. Otherwise, adults of A. argillacea showed lower oviposition on plants under water stress, and their larvae did not complete development fed non-Bt varieties cultivated under water stress condition. These results indicated that adults and larvae of A. argillacae do not recognize Bt-cotton plants to lay eggs and to feed, respectively. Further, cotton plants under water stress showed greater Cry1Ac toxin concentration on top leaves, interfered with the oviposition of A. argillacea irrespective of plant genotypes Bt and non-Bt, and with the larval development on non-Bt plants. |
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Torres, Jorge Brazhttp://lattes.cnpq.br/5131993649526120Lima, Iracilda Maria de MouraLIMA, I. M. M.Guzzo, Elio CesarGUZZO, Elio CesarTorres, Christian Sherley Araujo da Silvahttp://lattes.cnpq.br/6987761145766931http://lattes.cnpq.br/0031701044459853Lima, Mauricio Silva de2015-08-25T18:05:13Z2012-03-212015-08-25T18:05:13Z2010-03-31LIMA, Mauricio Silva de. Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae). 2010. 47 f. Dissertação (Mestrado em Agronomia:Produção Vegetal) - Centro de Ciências Agrárias, Programa de Pós Graduação em Agronomia, Universidade Federal de Alagoas, Rio Largo, 2010.http://repositorio.ufal.br/handle/riufal/249The genetically modified cotton to produce toxins from the bacterium Bacillus thuringiensis (Bt) has the objective of offering plant resistance against lepidopteran larvae. The cotton crop cultivated in the Semiarid region can be subjected to the condition of water stress. Thus, this work investigated the expression of the Cry1Ac toxin in two varieties of Bt-cotton Acala 90B and NuOpal cultivated under the conditions of irrigated and water stress. In addition, the oviposition and feeding preference of adults and larvae of the cotton leafworm, Alabama Argillacea (Hübner), caged on these plants were studied. Cotton plants were cultivated under controlled conditions of irrigation to obtain water stress treatment in microparcels inside an open-sided greenhouse. Higher levels of Cry1Ac expression was found on top leaves of Bt-cotton for both varieties under water stress condition, and similar levels between plants with and without water stress in the other plant parts such as bracts, flower petals, and boll. Adults of A. argillacea did not exhibited preference for oviposition between Bt and non-Bt cotton plants. Likewise, 3- and 10-d-old A. argillacea larvae did not show food preference under a choice test comparing leaves of non-Bt and Bt-cotton plants. Otherwise, adults of A. argillacea showed lower oviposition on plants under water stress, and their larvae did not complete development fed non-Bt varieties cultivated under water stress condition. These results indicated that adults and larvae of A. argillacae do not recognize Bt-cotton plants to lay eggs and to feed, respectively. Further, cotton plants under water stress showed greater Cry1Ac toxin concentration on top leaves, interfered with the oviposition of A. argillacea irrespective of plant genotypes Bt and non-Bt, and with the larval development on non-Bt plants.O algodoeiro geneticamente modificado para a produção de toxinas da bactéria Bacillus thuringiensis (=Bt) tem como principal objetivo oferecer a planta resistência contra larvas de lepidópteros. O cultivo do algodoeiro Bt no Semiárido pode estar submetido a diferentes condições edafoclimáticas, principalmente, ao estresse hídrico. Assim, este estudo investigou a produção da toxina Cry1Ac em algodoeiro Bt variedades Acala 90B e NuOpal com plantas submetidas ou não ao estresse hídrico e a preferência para oviposição e alimentação de Alabama Argillacea (Hübner) confinadas sobre essas plantas. Plantas de algodão foram cultivadas em microparcelas em casa telada e submetidas ao controle de irrigação para a obtenção de plantas com estresse hídrico. A produção de Cry1Ac foi maior em folhas de plantas Bt de ambas as variedades submetidas ao estresse hídrico, e similar nas demais partes como brácteas, pétalas e casca de maçãs. Mariposas de A. argillacea não apresentaram preferência para ovipositar entre plantas de algodão Bt e não-Bt. Da mesma forma, as larvas com três ou 10 dias de idade não apresentaram preferência para alimentar em folhas de variedades não-Bt comparadas a Bt, em testes com chance de escolha. Por outro lado, mariposas de A. argillacea preferiram ovipositar em plantas sem estresse hídrico e larvas não completaram o desenvolvimento em plantas não-Bt sob estresse hídrico de ambas as variedades. Estes resultados mostram que tanto mariposas quanto as larvas de A. argillacea não foram capazes de reconhecer plantas de algodão Bt para ovipositar e alimentar, respectivamente. Também, que plantas de algodoeiro submetidas ao estresse hídrico apresentam diferenças quanto à quantidade da toxina Cry1Ac nas folhas, interferiram com a preferência de oviposição de A. argillacea, bem como com o seu desenvolvimento larval.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de AlagoasPrograma de Pós-Graduação em AgronomiaUFALBRInsectaBehaviorHost plant resistanceCry toxinInsectaComportamentoResistência de plantasToxina CryCNPQ::CIENCIAS AGRARIAS::AGRONOMIAInteração do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae)Interaction to BT cotton submety and stress and Alabama argillacea (Hübner) (Lepidoptera: Noctuidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal de Alagoas (UFAL)instname:Universidade Federal de Alagoas (UFAL)instacron:UFALORIGINALInteração do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae).pdfapplication/pdf1526394http://www.repositorio.ufal.br/jspui/bitstream/riufal/249/1/Intera%c3%a7%c3%a3o%20do%20algodoeiro%20BT%20submetido%20ao%20estresse%20h%c3%addrico%20e%20Alabama%20argillacea%20%28H%c3%bcbner%29%20%28Lepidoptera%3a%20Noctuidae%29.pdf7e9ccd89c6fbcbbaae2fcba6d43c7d88MD51TEXTInteração do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae).txtInteração do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae).txtExtracted texttext/plain79561http://www.repositorio.ufal.br/jspui/bitstream/riufal/249/2/Intera%c3%a7%c3%a3o%20do%20algodoeiro%20BT%20submetido%20ao%20estresse%20h%c3%addrico%20e%20Alabama%20argillacea%20%28H%c3%bcbner%29%20%28Lepidoptera%3a%20Noctuidae%29.txt6f481a0d7f29b43415571a38232baa0dMD52riufal/2492018-04-25 02:29:06.426oai:www.repositorio.ufal.br:riufal/249Repositório InstitucionalPUBhttp://www.repositorio.ufal.br/oai/requestri@sibi.ufal.bropendoar:46482018-04-25T02:29:06Repositório Institucional da Universidade Federal de Alagoas (UFAL) - Universidade Federal de Alagoas (UFAL)false |
dc.title.por.fl_str_mv |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
dc.title.alternative.eng.fl_str_mv |
Interaction to BT cotton submety and stress and Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
title |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
spellingShingle |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) Lima, Mauricio Silva de Insecta Behavior Host plant resistance Cry toxin Insecta Comportamento Resistência de plantas Toxina Cry CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
title_full |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
title_fullStr |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
title_sort |
Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) |
author |
Lima, Mauricio Silva de |
author_facet |
Lima, Mauricio Silva de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Torres, Jorge Braz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5131993649526120 |
dc.contributor.referee1.fl_str_mv |
Lima, Iracilda Maria de Moura |
dc.contributor.referee1Lattes.fl_str_mv |
LIMA, I. M. M. |
dc.contributor.referee2.fl_str_mv |
Guzzo, Elio Cesar |
dc.contributor.referee2Lattes.fl_str_mv |
GUZZO, Elio Cesar |
dc.contributor.referee3.fl_str_mv |
Torres, Christian Sherley Araujo da Silva |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/6987761145766931 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0031701044459853 |
dc.contributor.author.fl_str_mv |
Lima, Mauricio Silva de |
contributor_str_mv |
Torres, Jorge Braz Lima, Iracilda Maria de Moura Guzzo, Elio Cesar Torres, Christian Sherley Araujo da Silva |
dc.subject.eng.fl_str_mv |
Insecta Behavior Host plant resistance Cry toxin |
topic |
Insecta Behavior Host plant resistance Cry toxin Insecta Comportamento Resistência de plantas Toxina Cry CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.por.fl_str_mv |
Insecta Comportamento Resistência de plantas Toxina Cry |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The genetically modified cotton to produce toxins from the bacterium Bacillus thuringiensis (Bt) has the objective of offering plant resistance against lepidopteran larvae. The cotton crop cultivated in the Semiarid region can be subjected to the condition of water stress. Thus, this work investigated the expression of the Cry1Ac toxin in two varieties of Bt-cotton Acala 90B and NuOpal cultivated under the conditions of irrigated and water stress. In addition, the oviposition and feeding preference of adults and larvae of the cotton leafworm, Alabama Argillacea (Hübner), caged on these plants were studied. Cotton plants were cultivated under controlled conditions of irrigation to obtain water stress treatment in microparcels inside an open-sided greenhouse. Higher levels of Cry1Ac expression was found on top leaves of Bt-cotton for both varieties under water stress condition, and similar levels between plants with and without water stress in the other plant parts such as bracts, flower petals, and boll. Adults of A. argillacea did not exhibited preference for oviposition between Bt and non-Bt cotton plants. Likewise, 3- and 10-d-old A. argillacea larvae did not show food preference under a choice test comparing leaves of non-Bt and Bt-cotton plants. Otherwise, adults of A. argillacea showed lower oviposition on plants under water stress, and their larvae did not complete development fed non-Bt varieties cultivated under water stress condition. These results indicated that adults and larvae of A. argillacae do not recognize Bt-cotton plants to lay eggs and to feed, respectively. Further, cotton plants under water stress showed greater Cry1Ac toxin concentration on top leaves, interfered with the oviposition of A. argillacea irrespective of plant genotypes Bt and non-Bt, and with the larval development on non-Bt plants. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-03-31 |
dc.date.available.fl_str_mv |
2012-03-21 2015-08-25T18:05:13Z |
dc.date.accessioned.fl_str_mv |
2015-08-25T18:05:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Mauricio Silva de. Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae). 2010. 47 f. Dissertação (Mestrado em Agronomia:Produção Vegetal) - Centro de Ciências Agrárias, Programa de Pós Graduação em Agronomia, Universidade Federal de Alagoas, Rio Largo, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufal.br/handle/riufal/249 |
identifier_str_mv |
LIMA, Mauricio Silva de. Interação do algodoeiro BT submetido ao estresse hídrico e Alabama argillacea (Hübner) (Lepidoptera: Noctuidae). 2010. 47 f. Dissertação (Mestrado em Agronomia:Produção Vegetal) - Centro de Ciências Agrárias, Programa de Pós Graduação em Agronomia, Universidade Federal de Alagoas, Rio Largo, 2010. |
url |
http://repositorio.ufal.br/handle/riufal/249 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Alagoas |
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Programa de Pós-Graduação em Agronomia |
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UFAL |
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BR |
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Universidade Federal de Alagoas |
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