Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT
Ano de defesa: | 2021 |
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Autor(a) principal: | |
Outros Autores: | , |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | eng |
Instituição de defesa: |
Universidade Federal do Amazonas
Instituto de Ciências Biológicas Brasil UFAM Programa de Pós-graduação em Imunologia Básica e Aplicada |
Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Link de acesso: | https://tede.ufam.edu.br/handle/tede/8399 |
Resumo: | Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation through a variety of mechanisms, including the production of pro-inflammatory and anti-inflammatory cytokines, which are directed by different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents inflammation when uncontrolled and the Amazon is rich in natural products that need to be studied. The objective of this study was to elucidate whether or not the anti-inflammatory effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS. Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the production of IL-10. The increased production of IL-10 was associated with elevated production of adenosine and this effect was not observed when the different steps of the adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The participation of the metabolism of the cells was also confirmed as the direct source of ATP for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the cells to generate the substrate for this pathway. |
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Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOTEfeitos anti-inflamatórios dependentes de adenosina de Arrabidaea Chica VERLOTPropriedades anti-inflamatóriasCitocina IL-1βMacrófagos derivados da medula ósseaAdenosinaDesfosforilação de ATPCIÊNCIAS BIOLÓGICASArrabidaea Chica VERLOTInflammationAnti-inflammatoryAdenosineATPIL-10IL-1βArrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation through a variety of mechanisms, including the production of pro-inflammatory and anti-inflammatory cytokines, which are directed by different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents inflammation when uncontrolled and the Amazon is rich in natural products that need to be studied. The objective of this study was to elucidate whether or not the anti-inflammatory effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS. Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the production of IL-10. The increased production of IL-10 was associated with elevated production of adenosine and this effect was not observed when the different steps of the adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The participation of the metabolism of the cells was also confirmed as the direct source of ATP for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the cells to generate the substrate for this pathway.Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation through a variety of mechanisms, including the production of pro-inflammatory and anti-inflammatory cytokines, which are directed by different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents inflammation when uncontrolled and the Amazon is rich in natural products that need to be studied. The objective of this study was to elucidate whether or not the anti-inflammatory effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS. Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the production of IL-10. The increased production of IL-10 was associated with elevated production of adenosine and this effect was not observed when the different steps of the adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The participation of the metabolism of the cells was also confirmed as the direct source of ATP for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the cells to generate the substrate for this pathway.Universidade Federal do AmazonasInstituto de Ciências BiológicasBrasilUFAMPrograma de Pós-graduação em Imunologia Básica e AplicadaAlves Filho, José Carlos Fariashttp://lattes.cnpq.br/1316491658346452Sadahiro, Ayahttp://lattes.cnpq.br/8658798733544812Corrêa, José Wilson do Nascimentohttp://lattes.cnpq.br/9130747433263550Silva, Rafael Brito dahttp://lattes.cnpq.br/5279568988466735Ubiera, Rosmery Duranhttp://lattes.cnpq.br/6310867371281249https://orcid.org/0000-0002-6764-245X2021-08-17T16:02:09Z2021-04-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfUBIERA, Rosmery Duran. Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT. 2021. 80 f. Tese (Doutorado em Imunologia Básica e Aplicada) - Universidade Federal do Amazonas, Manaus, 2021.https://tede.ufam.edu.br/handle/tede/8399enghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFAMinstname:Universidade Federal do Amazonas (UFAM)instacron:UFAM2021-08-18T05:03:34Zoai:https://tede.ufam.edu.br/handle/:tede/8399Biblioteca Digital de Teses e Dissertaçõeshttp://200.129.163.131:8080/PUBhttp://200.129.163.131:8080/oai/requestddbc@ufam.edu.br||ddbc@ufam.edu.bropendoar:65922021-08-18T05:03:34Biblioteca Digital de Teses e Dissertações da UFAM - Universidade Federal do Amazonas (UFAM)false |
dc.title.none.fl_str_mv |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT Efeitos anti-inflamatórios dependentes de adenosina de Arrabidaea Chica VERLOT |
title |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
spellingShingle |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT Ubiera, Rosmery Duran Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β |
title_short |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
title_full |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
title_fullStr |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
title_full_unstemmed |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
title_sort |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
author |
Ubiera, Rosmery Duran |
author_facet |
Ubiera, Rosmery Duran http://lattes.cnpq.br/6310867371281249 https://orcid.org/0000-0002-6764-245X |
author_role |
author |
author2 |
http://lattes.cnpq.br/6310867371281249 https://orcid.org/0000-0002-6764-245X |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Alves Filho, José Carlos Farias http://lattes.cnpq.br/1316491658346452 Sadahiro, Aya http://lattes.cnpq.br/8658798733544812 Corrêa, José Wilson do Nascimento http://lattes.cnpq.br/9130747433263550 Silva, Rafael Brito da http://lattes.cnpq.br/5279568988466735 |
dc.contributor.author.fl_str_mv |
Ubiera, Rosmery Duran http://lattes.cnpq.br/6310867371281249 https://orcid.org/0000-0002-6764-245X |
dc.subject.por.fl_str_mv |
Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β |
topic |
Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β |
description |
Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation through a variety of mechanisms, including the production of pro-inflammatory and anti-inflammatory cytokines, which are directed by different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents inflammation when uncontrolled and the Amazon is rich in natural products that need to be studied. The objective of this study was to elucidate whether or not the anti-inflammatory effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS. Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the production of IL-10. The increased production of IL-10 was associated with elevated production of adenosine and this effect was not observed when the different steps of the adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The participation of the metabolism of the cells was also confirmed as the direct source of ATP for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the cells to generate the substrate for this pathway. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-17T16:02:09Z 2021-04-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
UBIERA, Rosmery Duran. Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT. 2021. 80 f. Tese (Doutorado em Imunologia Básica e Aplicada) - Universidade Federal do Amazonas, Manaus, 2021. https://tede.ufam.edu.br/handle/tede/8399 |
identifier_str_mv |
UBIERA, Rosmery Duran. Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT. 2021. 80 f. Tese (Doutorado em Imunologia Básica e Aplicada) - Universidade Federal do Amazonas, Manaus, 2021. |
url |
https://tede.ufam.edu.br/handle/tede/8399 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Amazonas Instituto de Ciências Biológicas Brasil UFAM Programa de Pós-graduação em Imunologia Básica e Aplicada |
publisher.none.fl_str_mv |
Universidade Federal do Amazonas Instituto de Ciências Biológicas Brasil UFAM Programa de Pós-graduação em Imunologia Básica e Aplicada |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFAM instname:Universidade Federal do Amazonas (UFAM) instacron:UFAM |
instname_str |
Universidade Federal do Amazonas (UFAM) |
instacron_str |
UFAM |
institution |
UFAM |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFAM |
collection |
Biblioteca Digital de Teses e Dissertações da UFAM |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFAM - Universidade Federal do Amazonas (UFAM) |
repository.mail.fl_str_mv |
ddbc@ufam.edu.br||ddbc@ufam.edu.br |
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