Análise do desempenho de tecnologias alternativas para rastreio de lesões precursoras e de câncer invasivo de colo de útero

• Ano de defesa: 2019
• Autor(a) principal: Sena, Alysson Bastos
• Orientador(a): Torres, Kátia Luz
• Banca de defesa: Santos, Cristina Maria Borborema dos , Ramasawmy, Rajendranath
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Amazonas
• Programa de Pós-Graduação: Programa de Pós-graduação em Imunologia Básica e Aplicada
• Departamento: Instituto de Ciências Biológicas
• País: Brasil
• Palavras-chave em Português: Neoplasias do Colo do Útero; Infecções por Papillomavirus; Útero - Câncer - Amazonas; Programas de Rastreamento
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS
• Link de acesso: https://tede.ufam.edu.br/handle/tede/8094

Human Papillomavirus (HPV) infection is common among sexually active women, and viral persistence can lead to intraepithelial lesions that may progress to cervical cancer (CC). In women in the State of Amazonas, CC ranks first among cancers resulting in 288 deaths in 2018 from this cancer. Self-sampling of cervical material followed by HPV detection tests may be an alternative for women in isolation who have little or no access to health services in an attempt to increase coverage and the screening efficiency. This study analyzed alternative technologies using Coari® (Kolplast) and SelfCervix® (Ziel Biosciences) self-sampling devices followed by molecular analyzes by two generic PGMY9 /11 standard PCR assays and Real Time PCR for HPV16 and HPV 18. A total of 218 women with grade 2 or more advanced cervical intraepithelial neoplasia (NIC2; NIC3 or CC) were studied, with results confirmed by histopathological examination, who present themselves to start treatment at the CECON Foundation. Both devices had a higher acceptance level than those countries considering patients in sick conditions. It was recommended to use cervical screening by 98.2%. of the patients they used. In all lesions including CCU the predominance was 35> 45 years and 62.4% were positive for HPV according to the available methodology. All devices were viable and validated because they were independent of the collection method and sociodemographic factors. The presence of human DNA demonstrates the ability of the device to capture vaginal cervical cells.