Efeito da galactana sulfatada isolada da alga marinha Acanthophora muscoides sobre a adipogênese em células 3T3-L1

• Ano de defesa: 2021
• Autor(a) principal: Silva, Ana Virgínia Lima da
• Orientador(a): Santos, Flávia Almeida
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Adipogênese; Obesidade; Células 3T3-L1; Alga Marinha
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/60072

Obesity is a disease that is directly related to several metabolic disorders, becoming, consequently, one of the most costly challenges for public health today. The large number of adverse effects of anti-obesity drugs currently available has motivated the search for new drugs that are both therapeutically potent and safe. Thus, there has been a growing interest in natural products with anti-obesity activity. The objective of this study was to evaluate the effect of sulfated galactan from Acanthophora muscoides (GSAM) on adipogenesis in 3T3-L1 cells, by staining with Oil Red O and protein expression analysis of important transcription factors involved in this process, by Western Blot. The MTT assay showed that GSAM (6.25 – 400 μg/mL) caused a 22% reduction in the viability of 3T3-L1 cells, only at the concentration of 400 μg/mL. By staining with Oil Red O, it was observed that GSAM (25, 50 and 100 μg/mL) reduced the lipid content in 3T3-L1 cells by 21.6%, 28.5% and 40.5%, respectively, compared to the control group (D). Treatment with GSAM at concentrations of 50 and 100 μg/mL reduced the protein expression of C/EBPβ by 25.9% and 32.9%, respectively, compared to the control group (D). GSAM (25, 50 and 100 μg/mL) reduced the expressions of C/EBPδ, by 27.1%, 28.7% and 33.3%, and of the main adipogenic transcription factors, C/EBPα, by 17 .6%, 21.1% and 23.6%, and PPARγ, by 18.7%, 24.2% and 26%, respectively, compared to the control group (D). Protein expression of SREBP-1 was reduced by GSAM only at the concentration of 100 μg/mL, by 37.7%, and GSAM promoted an increase in AMPKα phosphorylation at concentrations of 50 and 100 μg/mL, by 31.8 % and 41.3%, respectively, compared to the control group (D). GSAM (25, 50 and 100 μg/ml) did not alter GLUT4 translocation, compared to the control group (D). Thus, it is concluded that GSAM inhibits the differentiation of 3T3-L1 cells by negatively regulating the expression of the main adipogenic transcription factors.