Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Silva Neto, Antonio Alves da
Orientador(a): Benevides, Norma Maria Barros
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/10488
Resumo: High energy demand and global climate changes have generated interest in world leaders to invest in research on alternative and renewable fuels. In this perspective, the macroalgae are gaining wide attention from researchers around the world as an alternative source of renewable biomass for bioethanol production, which is currently called fuel "third generation". The use of seaweed as a feedstock for bioethanol production has advantages such as (1) no competition with food production, (2) high carbohydrates content, (3) low lignin content and (4) high productivity. The potential of the red seaweed Hypnea musciformis to provide fermentable carbohydrates for bioethanol production was evaluated in this study. The algae was obtained from a commercial cultivation, located on the Flecheiras beach, Trairi, Ceará and after washing, drying and grinding 5 g were added to 100 mL HCl (0.2, 0.5 and 1.0 M) in Erlenmeyer flasks, autoclaved at 121 ºC (10, 20 and 30 min). It was observed the presence of galactose (7.4 to 10.8 g.L-1) and glucose (3.4 to 4.7 g.L-1) in all hydrolyzed and the hydrolysis condition 0.5/20, with a concentration of glucose + galactose 14.8 g.L-1, was selected for testing fermentation of monosaccharides by Saccharomyces cerevisiae at 30 ° C. The results showed that glucose and galactose were consumed simultaneously, however this consumption only started after 7 h of fermentation and after 52 h, 82.5% of glucose and 72% galactose had been consumed, with a maximum yield of 5.3 g.L-1 of ethanol, it represents a fermentation efficiency of 50% theory and showing the ability of S. cerevisiae ferment galactose from algal feedstock with a yield of 0.1 g ethanol/g dry seaweed. It was observed in the hydrolysis condition selected, a higher specific rate of the substrate consumption accompanied by the rate of ethanol production. The ethanol yields based on consumption of substrates (glucose + galactose) and biomass were 0.315 and 0.08 (g/g) respectively. The biomass and ethanol productivity were 0.008 g.L-1.h-1 and 0.100 g.L-1.h-1, respectively. With the date obtained it can be conclude that the red seaweed H. musciformis showed be a potential renewable source of biomass for the production of bioethanol. However, other studies are needed to optimize the production process of bioethanol from these organisms.
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spelling Silva Neto, Antonio Alves daBenevides, Norma Maria Barros2015-01-20T12:06:35Z2015-01-20T12:06:35Z2013SILVA NETO, A. A. Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol. 2013. 71 f. Dissertação (Mestrado em Bioquímica) - Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2013.http://www.repositorio.ufc.br/handle/riufc/10488High energy demand and global climate changes have generated interest in world leaders to invest in research on alternative and renewable fuels. In this perspective, the macroalgae are gaining wide attention from researchers around the world as an alternative source of renewable biomass for bioethanol production, which is currently called fuel "third generation". The use of seaweed as a feedstock for bioethanol production has advantages such as (1) no competition with food production, (2) high carbohydrates content, (3) low lignin content and (4) high productivity. The potential of the red seaweed Hypnea musciformis to provide fermentable carbohydrates for bioethanol production was evaluated in this study. The algae was obtained from a commercial cultivation, located on the Flecheiras beach, Trairi, Ceará and after washing, drying and grinding 5 g were added to 100 mL HCl (0.2, 0.5 and 1.0 M) in Erlenmeyer flasks, autoclaved at 121 ºC (10, 20 and 30 min). It was observed the presence of galactose (7.4 to 10.8 g.L-1) and glucose (3.4 to 4.7 g.L-1) in all hydrolyzed and the hydrolysis condition 0.5/20, with a concentration of glucose + galactose 14.8 g.L-1, was selected for testing fermentation of monosaccharides by Saccharomyces cerevisiae at 30 ° C. The results showed that glucose and galactose were consumed simultaneously, however this consumption only started after 7 h of fermentation and after 52 h, 82.5% of glucose and 72% galactose had been consumed, with a maximum yield of 5.3 g.L-1 of ethanol, it represents a fermentation efficiency of 50% theory and showing the ability of S. cerevisiae ferment galactose from algal feedstock with a yield of 0.1 g ethanol/g dry seaweed. It was observed in the hydrolysis condition selected, a higher specific rate of the substrate consumption accompanied by the rate of ethanol production. The ethanol yields based on consumption of substrates (glucose + galactose) and biomass were 0.315 and 0.08 (g/g) respectively. The biomass and ethanol productivity were 0.008 g.L-1.h-1 and 0.100 g.L-1.h-1, respectively. With the date obtained it can be conclude that the red seaweed H. musciformis showed be a potential renewable source of biomass for the production of bioethanol. However, other studies are needed to optimize the production process of bioethanol from these organisms.Alta demanda de energia e mudanças climáticas globais têm gerado interesse dos governantes mundiais para investir em pesquisas de fontes alternativas e renováveis de combustíveis. Nessa perspectiva, as macroalgas vêm ganhando ampla atenção por parte de pesquisadores do mundo inteiro como fonte alternativa renovável de biomassa para a produção de bioetanol, o qual é denominado atualmente de combustível de “terceira geração”. A utilização das algas marinhas como matéria-prima para produção de bioetanol apresenta vantagens, tais como (1) não competição com a produção de alimentos, (2) alto conteúdo de carboidratos, (3) baixo conteúdo de lignina e (4) alta produtividade. O potencial da alga marinha vermelha Hypnea musciformis em fornecer carboidratos fermentescíveis para a produção de bioetanol foi avaliado no presente trabalho. A alga foi obtida de cultivo comercial, localizado na praia de Flecheiras, município de Trairi, Ceará e após lavagem, secagem e trituração, 5 g foram adicionados a 100 mL de HCl (0,2; 0,5 e 1,0 M) em erlenmeyers, autoclavados a 121 ºC (10, 20 e 30 min). Foi observada a presença de galactose (7,4 – 10,8 g.L-1) e glucose (3,4 – 4,7 g.L-1) em todos os hidrolisados e a condição de hidrólise 0,5/20, apresentando uma concentração de glicose + galactose de 14,8 g.L-1, foi selecionada para os ensaios de fermentação dos monossacarídeos por Saccharomyces cerevisiae a 30°C. Os resultados mostraram que a glicose e a galactose, foram consumidas simultaneamente, no entanto esse consumo só foi iniciado após 7 h de fermentação e após 52 h, 82,5 % da glicose e 72% da galactose tinham sido consumidas, com uma produção máxima de 5,3 g.L-1 de bioetanol, representando uma eficiência fermentativa de 50% do teórico e evidenciando a habilidade da S. cerevisiae em fermentar a galactose proveniente de matéria-prima algácea com um rendimento de 0,1 g de bioetanol/g de alga seca. Observou-se, na condição de hidrólise selecionada, uma maior velocidade específica de consumo de substrato acompanhado da velocidade de produção de etanol. Os rendimentos de etanol baseados no consumo de substrato (glucose + galactose) e biomassa foram 0,315 e 0,08 (g/g), respectivamente. As produtividades de biomassa e etanol foram 0,008 g.L-1.h-1 e 0,100 g.L-1.h-1, respectivamente. Com os dados obtidos pode-se concluir que a alga marinha H. musciformis se mostrou uma potencial fonte renovável de biomassa para a produção de etanol. No entanto, são necessários mais estudos para otimizar o processo produtivo de bioetanol a partir desses organismos.BioquímicaBiocombustívelAlgas vermelhasLeveduraAlga marinhaFermentaçãoAlga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.Red seaweed Hypnea musciformis (Wulfen) as a potential source of carbohydrates for ethanol production.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81786http://repositorio.ufc.br/bitstream/riufc/10488/2/license.txt8c4401d3d14722a7ca2d07c782a1aab3MD52ORIGINAL2013_dis_aasilvaneto.pdf2013_dis_aasilvaneto.pdfapplication/pdf1454794http://repositorio.ufc.br/bitstream/riufc/10488/1/2013_dis_aasilvaneto.pdf02bd2d1d16caf7d1fd1fe2eee71ce722MD51riufc/104882020-05-20 16:15:10.901oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2020-05-20T19:15:10Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
dc.title.en.pt_BR.fl_str_mv Red seaweed Hypnea musciformis (Wulfen) as a potential source of carbohydrates for ethanol production.
title Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
spellingShingle Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
Silva Neto, Antonio Alves da
Bioquímica
Biocombustível
Algas vermelhas
Levedura
Alga marinha
Fermentação
title_short Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
title_full Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
title_fullStr Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
title_full_unstemmed Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
title_sort Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol.
author Silva Neto, Antonio Alves da
author_facet Silva Neto, Antonio Alves da
author_role author
dc.contributor.author.fl_str_mv Silva Neto, Antonio Alves da
dc.contributor.advisor1.fl_str_mv Benevides, Norma Maria Barros
contributor_str_mv Benevides, Norma Maria Barros
dc.subject.por.fl_str_mv Bioquímica
Biocombustível
Algas vermelhas
Levedura
Alga marinha
Fermentação
topic Bioquímica
Biocombustível
Algas vermelhas
Levedura
Alga marinha
Fermentação
description High energy demand and global climate changes have generated interest in world leaders to invest in research on alternative and renewable fuels. In this perspective, the macroalgae are gaining wide attention from researchers around the world as an alternative source of renewable biomass for bioethanol production, which is currently called fuel "third generation". The use of seaweed as a feedstock for bioethanol production has advantages such as (1) no competition with food production, (2) high carbohydrates content, (3) low lignin content and (4) high productivity. The potential of the red seaweed Hypnea musciformis to provide fermentable carbohydrates for bioethanol production was evaluated in this study. The algae was obtained from a commercial cultivation, located on the Flecheiras beach, Trairi, Ceará and after washing, drying and grinding 5 g were added to 100 mL HCl (0.2, 0.5 and 1.0 M) in Erlenmeyer flasks, autoclaved at 121 ºC (10, 20 and 30 min). It was observed the presence of galactose (7.4 to 10.8 g.L-1) and glucose (3.4 to 4.7 g.L-1) in all hydrolyzed and the hydrolysis condition 0.5/20, with a concentration of glucose + galactose 14.8 g.L-1, was selected for testing fermentation of monosaccharides by Saccharomyces cerevisiae at 30 ° C. The results showed that glucose and galactose were consumed simultaneously, however this consumption only started after 7 h of fermentation and after 52 h, 82.5% of glucose and 72% galactose had been consumed, with a maximum yield of 5.3 g.L-1 of ethanol, it represents a fermentation efficiency of 50% theory and showing the ability of S. cerevisiae ferment galactose from algal feedstock with a yield of 0.1 g ethanol/g dry seaweed. It was observed in the hydrolysis condition selected, a higher specific rate of the substrate consumption accompanied by the rate of ethanol production. The ethanol yields based on consumption of substrates (glucose + galactose) and biomass were 0.315 and 0.08 (g/g) respectively. The biomass and ethanol productivity were 0.008 g.L-1.h-1 and 0.100 g.L-1.h-1, respectively. With the date obtained it can be conclude that the red seaweed H. musciformis showed be a potential renewable source of biomass for the production of bioethanol. However, other studies are needed to optimize the production process of bioethanol from these organisms.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-01-20T12:06:35Z
dc.date.available.fl_str_mv 2015-01-20T12:06:35Z
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dc.identifier.citation.fl_str_mv SILVA NETO, A. A. Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol. 2013. 71 f. Dissertação (Mestrado em Bioquímica) - Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2013.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/10488
identifier_str_mv SILVA NETO, A. A. Alga marinha vermelha Hypnea musciformis (wulfen) como fonte potencial de carboidratos para a produção de etanol. 2013. 71 f. Dissertação (Mestrado em Bioquímica) - Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2013.
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