Proteoma do longissimus dorsi de bovinos de corte inteiros e imuno castrados em sistema de suplementação à pasto

• Ano de defesa: 2018
• Autor(a) principal: Sousa, Isabela Christie dos Santos
• Orientador(a): Moura, Arlindo de Alencar Araripe Noronha
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Proteômica; Imunocastração; Bos taurus; Qualidade da carne
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/69003

The study was carried out with the aim of identifying the changes in the composition of protein of the muscle Longissimus dorsi, by electrophoresis one-dimensional, of bovine animals of different conditions sex and genetic groups. Were used 18 steers of the breed Nelore and 16 cattle crossbred (½ blood Dutch ½ Zebu), a total of 34 samples. After the end of the experiment the pasture, these animals were slaughtered and samples of muscle Longissimus dorsi were taken for proteomic analysis of tissue. The samples were grouped by breed and treatment as follows: Nellore without imunocastração or integers (NI), Nellore imunocastrado (NIC), mestizo integer (MI) and mestizo imunocastrado (MIC). The samples were freeze dried, macerated and homogenized in buffer solution. The supernatant was reserved and the amount of protein determined by the method of Bradford (1976). The separation was made in the 1st dimension in a system of isoelectric focalization in polyacrylamide gel of 12.5%. The gels obtained were stained using Comassie Blue (R-250) and subsequently bleached with a solution containing methanol (40%) and acetic acid (10%). After discoloration, the gel was scanned using the application ScannerImagem (GE Healthcare, Piscataway, NJ, USA). The proteins separated by 1D PAGE were subjected to digestion with trypsin and then were identified by mass spectrometry. Of the 35 bands excisadas detected 263 proteins. By comparing the expression of the intensity of the bands between the treatments can be observed a statistically significant difference in the intensity of the 20 bands in function of the treatments, being accounted for 31 distinct proteins. Of this total, seven proteins were selected on the basis of their expression in the different treatments. The proteins identified in this experiment can be classified mainly as cytoplasmic, cellular process and regulation, as well as the catalytic activity. The analysis of the proteome can be a very effective method used to identify the protein marker for meat quality when compared to animals of differences races and sexual condition.