Contribuições ao desenvolvimento de fitoterápico a partir de Justicia pectoralis (chambá) para o tratamento da asma: otimização, caracterização e atividade anti-inflamatória

• Ano de defesa: 2018
• Autor(a) principal: Silva, Aline Holanda
• Orientador(a): Leal, Luzia Kalyne Almeida Moreira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Justicia; Asma; Micropartículas Derivadas de Células
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/37861

Justicia pectoralis Jacq. (Acanthaceae), “chambá”, it used in the treatment of respiratory diseases. Present in Pharmacies Vivas and Renisus, pharmacological studies demonstrate their anti-inflammatory, bronchodilator and antiasthmatic actions, being these related to the presence of coumarin (CM) and umbelliferone (UMB) in the plant. The aim of the study was to develop standardized dry extract (microparticles) and investigate its anti-inflammatory effect in an experimental model of exacerbated asthma.. Analytical methodology (CLAE-DAD) was validated for CM and UMB dosing and showed to be specific, linear, precise, accurate and robust. It was determined that the best harvest time of the aerial parts of J. pectoralis is at three months of cultivation, and seasonal influence is verified. In the optimization of the extraction process, after investigation of several methods and process variables, it was verified that the maceration followed by percolation had the best concentration of markers. The extract was used to optimize the production of the dry extract of Justicia pectoralis by spray drying (ESPJP), presenting good rheological indexes, low hygroscopicide, spherical morphology, CM = 21.10 ± 0.014 (0.70) and UMB = 1.74 ± 0.20 (1.05) and yield of 59.45 ± 1.78 (2.05). Chromatographic study (HPLC-DAD) and structural elucidation allowed to propose 2-O-rhamnosyl-eswertine as a new chemical marker. ESPJP did not show cytotoxicity (MTT and LDH) in human neutrophils. Anti-inflammatory effect of ESPJP was investigated in an experimental model of bronchial asthma exacerbated by immuno-infectious stimulus (OVA and LPS) in BALB/c mice. ESPJP (100 mg/Kg) was able to promote a reduction in the number of total cells present in BAL, as well as the activity of myeloperoxidase. Histological analysis revealed that ESPJP caused a decrease in the number of inflammatory cells in the peribronchial region and in the production of mucus in the lungs. Results allowed to define and standardize stages and parameters of ESPJP production that showed anti-inflammatory activity and was used in the formulation of phytotherapic for oral use.