Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Correia, Giovanna Riello Barbosa
Orientador(a): Brilhante , Raimunda Sâmia Nogueira
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Burkholderia pseudomallei
Biofilmes
Farneseno Álcool
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/16552
Resumo: The intrinsic antimicrobial resistance of Burkholderia pseudomallei is a serious challenge to the treatment of melioidosis. Many studies have searched for adjuvants that increase susceptibility bacteria to antimicrobials. In this context, the antimicrobial activity of farnesol against B. pseudomallei in planktonic growth has been reported. Thus, the aim of this study was to analyze the in vitro activity of farnesol alone against Burkholderia pseudomallei biofilms, as well as its combination with the antibacterials amoxicillin, doxycycline, ceftazidime and sulfamethoxazole-trimethoprim. Susceptibility was assessed by the broth microdilution test and cell viability was read with the oxidation-reduction indicator dye resazurin. The interaction between farnesol and antibacterial drugs against B. pseudomallei biofilms was evaluated through the calculation of the fractional inhibitory concentration index. The minimum biofilm erradication concentration (MBEC) for farnesol was 75 to 2400 mM. In addition, farnesol significantly reduced the MBEC values for ceftazidime,amoxicillin, doxycycline and sulfamethoxazole-trimethoprim by 256, 16, 4 and 4 times respectively (P<0.05). Optical, confocal and electronic microscopic analyses of farnesol treated B. pseudomallei biofilms demonstrated that this compound damages biofilm matrix,facilitating antimicrobial penetration in the biofilm structure. This study demonstrated the effectiveness of farnesol against B. pseudomallei biofilms and its potentiating effect on the activity of antibacterial drugs, in particular ceftazidime, amoxicillin, doxycycline and sulfamethoxazole-trimethoprim.
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spelling Correia, Giovanna Riello BarbosaBrilhante , Raimunda Sâmia Nogueira2016-05-02T15:59:07Z2016-05-02T15:59:07Z2015-07-14CORREIA, G. R. B. Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei. 2015. 88 f. Dissertação (Mestrado em Microbiologia Médica) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2015.http://www.repositorio.ufc.br/handle/riufc/16552The intrinsic antimicrobial resistance of Burkholderia pseudomallei is a serious challenge to the treatment of melioidosis. Many studies have searched for adjuvants that increase susceptibility bacteria to antimicrobials. In this context, the antimicrobial activity of farnesol against B. pseudomallei in planktonic growth has been reported. Thus, the aim of this study was to analyze the in vitro activity of farnesol alone against Burkholderia pseudomallei biofilms, as well as its combination with the antibacterials amoxicillin, doxycycline, ceftazidime and sulfamethoxazole-trimethoprim. Susceptibility was assessed by the broth microdilution test and cell viability was read with the oxidation-reduction indicator dye resazurin. The interaction between farnesol and antibacterial drugs against B. pseudomallei biofilms was evaluated through the calculation of the fractional inhibitory concentration index. The minimum biofilm erradication concentration (MBEC) for farnesol was 75 to 2400 mM. In addition, farnesol significantly reduced the MBEC values for ceftazidime,amoxicillin, doxycycline and sulfamethoxazole-trimethoprim by 256, 16, 4 and 4 times respectively (P<0.05). Optical, confocal and electronic microscopic analyses of farnesol treated B. pseudomallei biofilms demonstrated that this compound damages biofilm matrix,facilitating antimicrobial penetration in the biofilm structure. This study demonstrated the effectiveness of farnesol against B. pseudomallei biofilms and its potentiating effect on the activity of antibacterial drugs, in particular ceftazidime, amoxicillin, doxycycline and sulfamethoxazole-trimethoprim.A intrínseca resistência apresentada pela bactéria Burkholderia pseudomallei é um grave empecilho para o tratamento da melioidose. Muitas pesquisas focam na busca de adjuvantes que aumentem a sensibilidade desta bactéria aos antimicrobianos. Nesse contexto, a ação inibitória do farnesol frente às cepas de B. pseudomallei, na forma planctônica, já foi relatada em estudo prévio. Diante disso, esse estudo objetivou analisar a atividade in vitro do farnesol contra cepas de B. pseudomallei na forma de biofilme. Aliado à análise da ação do farnesol isoladamente, foi investigada a combinação desse composto com os antimicrobianos amoxicilina, ceftazidima, doxiciclina, imipenem e sulfametoxazol/trimetoprim frente ao biofilme. A sensibilidade foi avaliada por meio do teste de microdiluição em caldo e a leitura da viabilidade celular feita com a resazurina. A concentração inibitória mínima em biofilme (CEMB) para o farnesol foi de 75 a 2400 mM. Ademais, o farnesol reduziu em até 256, 16, 4 e 4 vezes os valores de CEMB para ceftazidima, amoxicilina, doxiciclina e sulfametoxazol/trimetoprim, respectivamente (P<0.05). Por meio de técnicas de microscopia, tais como óptica, confocal e eletrônica, observou-se que o farnesol foi capaz de causar danos na matriz do biofilme, facilitando assim, a penetração dos antibióticos. Deste modo, o presente estudo mostrou a eficácia do farnesol contra biofilmes de B. pseudomallei e seu efeito potenciador, em especial com ceftazidima, amoxicilina, doxiciclina e sulfametoxazol/trimetoprim.Burkholderia pseudomalleiBiofilmesFarneseno ÁlcoolEfeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomalleiInhibitory effect in vitro of farnesol against Burkholderia pseudomallei biofilmsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessORIGINAL2015_dis_grbcorreia.pdf2015_dis_grbcorreia.pdfapplication/pdf1260823http://repositorio.ufc.br/bitstream/riufc/16552/1/2015_dis_grbcorreia.pdf8ebde2fdd525176303b72981cc66cfa0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/16552/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufc/165522021-02-04 11:07:08.822oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2021-02-04T14:07:08Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
dc.title.en.pt_BR.fl_str_mv Inhibitory effect in vitro of farnesol against Burkholderia pseudomallei biofilms
title Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
spellingShingle Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
Correia, Giovanna Riello Barbosa
Burkholderia pseudomallei
Biofilmes
Farneseno Álcool
title_short Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
title_full Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
title_fullStr Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
title_full_unstemmed Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
title_sort Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei
author Correia, Giovanna Riello Barbosa
author_facet Correia, Giovanna Riello Barbosa
author_role author
dc.contributor.author.fl_str_mv Correia, Giovanna Riello Barbosa
dc.contributor.advisor1.fl_str_mv Brilhante , Raimunda Sâmia Nogueira
contributor_str_mv Brilhante , Raimunda Sâmia Nogueira
dc.subject.por.fl_str_mv Burkholderia pseudomallei
Biofilmes
Farneseno Álcool
topic Burkholderia pseudomallei
Biofilmes
Farneseno Álcool
description The intrinsic antimicrobial resistance of Burkholderia pseudomallei is a serious challenge to the treatment of melioidosis. Many studies have searched for adjuvants that increase susceptibility bacteria to antimicrobials. In this context, the antimicrobial activity of farnesol against B. pseudomallei in planktonic growth has been reported. Thus, the aim of this study was to analyze the in vitro activity of farnesol alone against Burkholderia pseudomallei biofilms, as well as its combination with the antibacterials amoxicillin, doxycycline, ceftazidime and sulfamethoxazole-trimethoprim. Susceptibility was assessed by the broth microdilution test and cell viability was read with the oxidation-reduction indicator dye resazurin. The interaction between farnesol and antibacterial drugs against B. pseudomallei biofilms was evaluated through the calculation of the fractional inhibitory concentration index. The minimum biofilm erradication concentration (MBEC) for farnesol was 75 to 2400 mM. In addition, farnesol significantly reduced the MBEC values for ceftazidime,amoxicillin, doxycycline and sulfamethoxazole-trimethoprim by 256, 16, 4 and 4 times respectively (P<0.05). Optical, confocal and electronic microscopic analyses of farnesol treated B. pseudomallei biofilms demonstrated that this compound damages biofilm matrix,facilitating antimicrobial penetration in the biofilm structure. This study demonstrated the effectiveness of farnesol against B. pseudomallei biofilms and its potentiating effect on the activity of antibacterial drugs, in particular ceftazidime, amoxicillin, doxycycline and sulfamethoxazole-trimethoprim.
publishDate 2015
dc.date.issued.fl_str_mv 2015-07-14
dc.date.accessioned.fl_str_mv 2016-05-02T15:59:07Z
dc.date.available.fl_str_mv 2016-05-02T15:59:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv CORREIA, G. R. B. Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei. 2015. 88 f. Dissertação (Mestrado em Microbiologia Médica) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2015.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/16552
identifier_str_mv CORREIA, G. R. B. Efeito inibitório in vitro do composto farnesol frente ao biofilme de Burkholderia pseudomallei. 2015. 88 f. Dissertação (Mestrado em Microbiologia Médica) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2015.
url http://www.repositorio.ufc.br/handle/riufc/16552
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reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
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