Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Lima, Ana Carolina Viana de
Orientador(a): Rodrigues, Maria do Carmo Passos
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/57721
Resumo: The pitaya (Hylocereus polyrhizus) has been developing very well in Brazil, in states like São Paulo, Rio Grande do Sul, Santa Catarina and Ceará, thus facilitating the cultivation and commercialization throughout the country. Some characteristics of this fruit have been attracting the attention of consumers and researchers, such as the mineral content, presence of antioxidant compounds and mainly its attractive coloring, this being linked to the high content of betalains. Thus, we can consider its potential use as a natural dye in order to replace artificial dyes used commercially, which when used above the allowed value has been causing problems to the health of consumers. Thus, this study aimed to characterize dye of pitaia, from the species Hylocereus polyrhizus, to evaluate its storage stability through chemical, physical-chemical, microbiological analyzes and using UPLC-ESI-QTOF-MSE techniques combined with statistical tools, in addition to verify their potential application in yoghurts by means of sensory tests and compare them with commercial products that use carmine or beet dye as food coloring in their formulations. The results showed that UPLC-ESI-QTOF-MSE was effective for the simultaneous determination of twenty metabolites, putatively identified as carbohydrates, flavonoids, and betalains. The MRPPC was shown to have microbiological and physicochemical stability through twelve weeks of storage, and chemometric analyses efficiently distinguished the metabolic profile in each storage period. Sensory analysis revealed that the MRPPC was effective as a food colorant in yogurt, where it improved color
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spelling Lima, Ana Carolina Viana deDionísio, Ana PaulaRodrigues, Maria do Carmo Passos2021-04-14T12:57:59Z2021-04-14T12:57:59Z2020LIMA, Ana Carolina Viana de. Corante de Pitaia vermelho-púrpura natural: perfil metabólico, aspectos tecnológicos e aplicação em alimentos. 2020. 70 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade Federal do Ceará, Fortaleza, 2020.http://www.repositorio.ufc.br/handle/riufc/57721The pitaya (Hylocereus polyrhizus) has been developing very well in Brazil, in states like São Paulo, Rio Grande do Sul, Santa Catarina and Ceará, thus facilitating the cultivation and commercialization throughout the country. Some characteristics of this fruit have been attracting the attention of consumers and researchers, such as the mineral content, presence of antioxidant compounds and mainly its attractive coloring, this being linked to the high content of betalains. Thus, we can consider its potential use as a natural dye in order to replace artificial dyes used commercially, which when used above the allowed value has been causing problems to the health of consumers. Thus, this study aimed to characterize dye of pitaia, from the species Hylocereus polyrhizus, to evaluate its storage stability through chemical, physical-chemical, microbiological analyzes and using UPLC-ESI-QTOF-MSE techniques combined with statistical tools, in addition to verify their potential application in yoghurts by means of sensory tests and compare them with commercial products that use carmine or beet dye as food coloring in their formulations. The results showed that UPLC-ESI-QTOF-MSE was effective for the simultaneous determination of twenty metabolites, putatively identified as carbohydrates, flavonoids, and betalains. The MRPPC was shown to have microbiological and physicochemical stability through twelve weeks of storage, and chemometric analyses efficiently distinguished the metabolic profile in each storage period. Sensory analysis revealed that the MRPPC was effective as a food colorant in yogurt, where it improved colorA pitaia (Hylocereus polyrhizus) vem se desenvolvendo muito bem no Brasil, em estados como São Paulo, Rio Grande do Sul, Santa Catarina e Ceará, facilitando assim o cultivo e a comercialização por todo o país. Algumas características desse fruto vêm chamando a atenção de consumidores e pesquisadores, como o conteúdo de minerais, presença de compostos antioxidantes e principalmente a sua coloração atraente, está estando ligado ao alto teor de betalaínas. Desta forma podemos considerar seu uso potencial como corante natural a fim de substituir corantes artificiais utilizados comercialmente, que quando usados acima do valor permitido vem causando problemas à saúde dos consumidores. Assim, este estudo objetivou caracterizar corante de pitaia, oriundo da espécie Hylocereus polyrhizus, avaliar sua estabilidade de armazenamento por meio de análises químicas, físico-químicas, microbiológicas e utilizando técnicas de UPLC-ESI-QTOF-MSE combinada com ferramentas estatísticas, além de verificar sua potencial aplicação em iogurtes por meios de testes sensoriais e compará-los com produtos comerciais que utilizam corante carmim ou beterraba como corante alimentar em suas formulações. Os resultados mostraram que o UPLC-ESI-QTOF-MSE foi eficaz para a determinação simultânea de vinte metabólitos, identificados como carboidratos, flavonoides e betalaínas. O CPVPM mostrou estabilidade microbiológica e físico-química até doze semanas de armazenamento e as análises quimiométricas distinguiram eficientemente o perfil metabólico em cada período de armazenamento. A análise sensorial revelou que o CPVPM foi eficiente como corante alimentar, especialmente em iogurte, melhorando a qualidade da cor sem afetar o aroma e outras características. Estes resultados indicam que o CPVPM, um corante natural, é um ingrediente alimentar promissor como corante vermelho-púrpura natural.Hylocereus polyrhizus (F.A.C. Weber) Britton & RoseUPLC-ESI-QTOF-MSeEstabilidadeMicrofiltraçãoIogurteCorante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentosRed-purple natural pitaya colorant: metabolic profile, technological aspects and food applicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/57721/4/license.txt8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINAL2020_dis_acvlima.pdf2020_dis_acvlima.pdfapplication/pdf1406022http://repositorio.ufc.br/bitstream/riufc/57721/3/2020_dis_acvlima.pdf53e17c4808102efc2b02ec606fee066aMD53riufc/577212021-04-14 09:59:25.042oai:repositorio.ufc.br:riufc/57721Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2021-04-14T12:59:25Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
dc.title.en.pt_BR.fl_str_mv Red-purple natural pitaya colorant: metabolic profile, technological aspects and food application
title Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
spellingShingle Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
Lima, Ana Carolina Viana de
Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose
UPLC-ESI-QTOF-MSe
Estabilidade
Microfiltração
Iogurte
title_short Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
title_full Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
title_fullStr Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
title_full_unstemmed Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
title_sort Corante de Pitaia vermelho-púrpura natural: perfil metabolômico, aspectos tecnológicos e aplicação em alimentos
author Lima, Ana Carolina Viana de
author_facet Lima, Ana Carolina Viana de
author_role author
dc.contributor.co-advisor.none.fl_str_mv Dionísio, Ana Paula
dc.contributor.author.fl_str_mv Lima, Ana Carolina Viana de
dc.contributor.advisor1.fl_str_mv Rodrigues, Maria do Carmo Passos
contributor_str_mv Rodrigues, Maria do Carmo Passos
dc.subject.por.fl_str_mv Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose
UPLC-ESI-QTOF-MSe
Estabilidade
Microfiltração
Iogurte
topic Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose
UPLC-ESI-QTOF-MSe
Estabilidade
Microfiltração
Iogurte
description The pitaya (Hylocereus polyrhizus) has been developing very well in Brazil, in states like São Paulo, Rio Grande do Sul, Santa Catarina and Ceará, thus facilitating the cultivation and commercialization throughout the country. Some characteristics of this fruit have been attracting the attention of consumers and researchers, such as the mineral content, presence of antioxidant compounds and mainly its attractive coloring, this being linked to the high content of betalains. Thus, we can consider its potential use as a natural dye in order to replace artificial dyes used commercially, which when used above the allowed value has been causing problems to the health of consumers. Thus, this study aimed to characterize dye of pitaia, from the species Hylocereus polyrhizus, to evaluate its storage stability through chemical, physical-chemical, microbiological analyzes and using UPLC-ESI-QTOF-MSE techniques combined with statistical tools, in addition to verify their potential application in yoghurts by means of sensory tests and compare them with commercial products that use carmine or beet dye as food coloring in their formulations. The results showed that UPLC-ESI-QTOF-MSE was effective for the simultaneous determination of twenty metabolites, putatively identified as carbohydrates, flavonoids, and betalains. The MRPPC was shown to have microbiological and physicochemical stability through twelve weeks of storage, and chemometric analyses efficiently distinguished the metabolic profile in each storage period. Sensory analysis revealed that the MRPPC was effective as a food colorant in yogurt, where it improved color
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-04-14T12:57:59Z
dc.date.available.fl_str_mv 2021-04-14T12:57:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv LIMA, Ana Carolina Viana de. Corante de Pitaia vermelho-púrpura natural: perfil metabólico, aspectos tecnológicos e aplicação em alimentos. 2020. 70 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade Federal do Ceará, Fortaleza, 2020.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/57721
identifier_str_mv LIMA, Ana Carolina Viana de. Corante de Pitaia vermelho-púrpura natural: perfil metabólico, aspectos tecnológicos e aplicação em alimentos. 2020. 70 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade Federal do Ceará, Fortaleza, 2020.
url http://www.repositorio.ufc.br/handle/riufc/57721
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