Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).

Detalhes bibliográficos
Ano de defesa: 2021
Autor(a) principal: Alves, Antônio Willame da Silva
Orientador(a): Rocha, Bruno Anderson Matias da
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Macroalga marinha verde
Lectina de ligação à galactose
Ácido siálico
Canais TRP
Espécies reativas de oxigênio
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/60000
Resumo: Inflammation and oxidative stress are processes associated with different human diseases. They are treated using drugs that have several side effects. Seaweed are sources of potentially relevant natural compounds for use as a therapeutic treatment of these disorders. Lectins are able to reversibly interact with complex carbohydrates and modulate cell membrane glycosylated receptors through this interaction. This study aimed to determine the antinociceptive and anti-inflammatory potential of CiL-1 in adult zebrafish by modulation of TRPA1 through lectin-glycan binding. Nociception was induced by formalin, and inflammation was induced by carrageenan. ROS content was determined using DCFH-DA. Possible neuromodulation by TRPA1 channel was also evaluated by camphor pretreatment. CiL-1 was efficacious at all tested doses, revealing anti-nociceptive and anti-inflammatory effects in adult zebrafish. This galactose-binding lectin was also able to reduce the content of ROS in brain and liver. In silico analyses showed CiL-1 interactions with both ligands tested. Oligosaccharide-1 (LacNac2) presents the most favorable binding energy with the protein. The interaction occurs at 4 subsites as an extended conformation at the site. Oligosaccharide-2 (LacNac2-Sia) had a less favorable curved-shape interaction energy. Based on the predictions made for the oligosaccharides, a tetra-antenate putative glycan was schematically constructed, illustrating an interaction between TRPA1 N-glycan and CiL-1. This binding seems to be related to CiL-1 anti-inflammatory activity as result of receptor modulation.
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spelling Alves, Antônio Willame da SilvaRocha, Bruno Anderson Matias da2021-08-18T13:20:19Z2021-08-18T13:20:19Z2021ALVES, Antônio Willame da Silva. Codium isthmocladum lectin 1 (CiL-1): Interaction with N-glycans explains antinociceptive and anti-inflammatory activities in adult zebrafish (Danio rerio). 2021. 104 f. Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza, 2021http://www.repositorio.ufc.br/handle/riufc/60000Inflammation and oxidative stress are processes associated with different human diseases. They are treated using drugs that have several side effects. Seaweed are sources of potentially relevant natural compounds for use as a therapeutic treatment of these disorders. Lectins are able to reversibly interact with complex carbohydrates and modulate cell membrane glycosylated receptors through this interaction. This study aimed to determine the antinociceptive and anti-inflammatory potential of CiL-1 in adult zebrafish by modulation of TRPA1 through lectin-glycan binding. Nociception was induced by formalin, and inflammation was induced by carrageenan. ROS content was determined using DCFH-DA. Possible neuromodulation by TRPA1 channel was also evaluated by camphor pretreatment. CiL-1 was efficacious at all tested doses, revealing anti-nociceptive and anti-inflammatory effects in adult zebrafish. This galactose-binding lectin was also able to reduce the content of ROS in brain and liver. In silico analyses showed CiL-1 interactions with both ligands tested. Oligosaccharide-1 (LacNac2) presents the most favorable binding energy with the protein. The interaction occurs at 4 subsites as an extended conformation at the site. Oligosaccharide-2 (LacNac2-Sia) had a less favorable curved-shape interaction energy. Based on the predictions made for the oligosaccharides, a tetra-antenate putative glycan was schematically constructed, illustrating an interaction between TRPA1 N-glycan and CiL-1. This binding seems to be related to CiL-1 anti-inflammatory activity as result of receptor modulation.A inflamação e o estresse oxidativo são processos associados a diferentes doenças humanas. São tratadas com o uso de drogas que apresentam vários efeitos colaterais. As macroalgas marinhas são fontes de compostos naturais potencialmente relevantes para uso como tratamento terapêutico dessas desordens. As lectinas têm capacidade de interagir reversivelmente com carboidratos complexos e modular os receptores glicosilados da membrana celular por meio dessa interação. Este estudo teve como objetivo determinar o potencial antinociceptivo e antiinflamatório de CiL-1 em peixes-zebra adultos pela modulação do TRPA1 por meio da ligação lectina-glicano. A nocicepção foi induzida por formalina e a inflamação foi induzida por carragenina. O conteúdo de ROS foi determinado usando DCFH-DA. A possível neuromodulação pelo canal TRPA1 também foi avaliada por pré-tratamento com cânfora. CiL-1 foi eficaz em todas as doses testadas, revelando efeitos antinociceptivos e antiinflamatórios em peixes-zebra adultos. Esta lectina de ligação à galactose também foi capaz de reduzir o conteúdo de ROS no cérebro e no fígado. Análises in silico mostraram interações de CiL-1 com ambos os ligantes testados. Oligossacarídeo-1 (LacNac2) apresenta a energia de ligação mais favorável com a proteína. A interação ocorre em 4 subsites como uma conformação estendida no site. O oligossacarídeo-2 (LacNac2-Sia) teve a curva de interação energética menos favorável. Com base nas previsões feitas para os oligossacarídeos, um glicano putativo tetra-antenato foi esquematicamente construído, ilustrando uma interação entre TRPA1 N-glicano e CiL-1. Essa ligação parece estar relacionada à atividade antiinflamatória da CiL-1 como resultado da modulação do receptor.Macroalga marinha verdeLectina de ligação à galactoseÁcido siálicoCanais TRPEspécies reativas de oxigênioLectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).Codium isthmocladum lectin 1 (CiL-1): Interaction with N-glycans explains antinociceptive and anti-inflammatory activities in adult zebrafish (Danio rerio)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/60000/4/license.txt8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINAL2021_tese_awsalves.pdf2021_tese_awsalves.pdfapplication/pdf7096310http://repositorio.ufc.br/bitstream/riufc/60000/3/2021_tese_awsalves.pdf8e108e45b3ad53b30e9274f0b13d2926MD53riufc/600002021-08-18 10:20:19.883oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2021-08-18T13:20:19Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
dc.title.en.pt_BR.fl_str_mv Codium isthmocladum lectin 1 (CiL-1): Interaction with N-glycans explains antinociceptive and anti-inflammatory activities in adult zebrafish (Danio rerio)
title Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
spellingShingle Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
Alves, Antônio Willame da Silva
Macroalga marinha verde
Lectina de ligação à galactose
Ácido siálico
Canais TRP
Espécies reativas de oxigênio
title_short Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
title_full Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
title_fullStr Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
title_full_unstemmed Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
title_sort Lectina Codium isthmocladum 1 (CiL-1): Interações com N-glicanos explicam atividades antinociceptiva e anti-inflamatória em zebrafish adulto (Danio rerio).
author Alves, Antônio Willame da Silva
author_facet Alves, Antônio Willame da Silva
author_role author
dc.contributor.author.fl_str_mv Alves, Antônio Willame da Silva
dc.contributor.advisor1.fl_str_mv Rocha, Bruno Anderson Matias da
contributor_str_mv Rocha, Bruno Anderson Matias da
dc.subject.por.fl_str_mv Macroalga marinha verde
Lectina de ligação à galactose
Ácido siálico
Canais TRP
Espécies reativas de oxigênio
topic Macroalga marinha verde
Lectina de ligação à galactose
Ácido siálico
Canais TRP
Espécies reativas de oxigênio
description Inflammation and oxidative stress are processes associated with different human diseases. They are treated using drugs that have several side effects. Seaweed are sources of potentially relevant natural compounds for use as a therapeutic treatment of these disorders. Lectins are able to reversibly interact with complex carbohydrates and modulate cell membrane glycosylated receptors through this interaction. This study aimed to determine the antinociceptive and anti-inflammatory potential of CiL-1 in adult zebrafish by modulation of TRPA1 through lectin-glycan binding. Nociception was induced by formalin, and inflammation was induced by carrageenan. ROS content was determined using DCFH-DA. Possible neuromodulation by TRPA1 channel was also evaluated by camphor pretreatment. CiL-1 was efficacious at all tested doses, revealing anti-nociceptive and anti-inflammatory effects in adult zebrafish. This galactose-binding lectin was also able to reduce the content of ROS in brain and liver. In silico analyses showed CiL-1 interactions with both ligands tested. Oligosaccharide-1 (LacNac2) presents the most favorable binding energy with the protein. The interaction occurs at 4 subsites as an extended conformation at the site. Oligosaccharide-2 (LacNac2-Sia) had a less favorable curved-shape interaction energy. Based on the predictions made for the oligosaccharides, a tetra-antenate putative glycan was schematically constructed, illustrating an interaction between TRPA1 N-glycan and CiL-1. This binding seems to be related to CiL-1 anti-inflammatory activity as result of receptor modulation.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-08-18T13:20:19Z
dc.date.available.fl_str_mv 2021-08-18T13:20:19Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv ALVES, Antônio Willame da Silva. Codium isthmocladum lectin 1 (CiL-1): Interaction with N-glycans explains antinociceptive and anti-inflammatory activities in adult zebrafish (Danio rerio). 2021. 104 f. Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza, 2021
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/60000
identifier_str_mv ALVES, Antônio Willame da Silva. Codium isthmocladum lectin 1 (CiL-1): Interaction with N-glycans explains antinociceptive and anti-inflammatory activities in adult zebrafish (Danio rerio). 2021. 104 f. Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza, 2021
url http://www.repositorio.ufc.br/handle/riufc/60000
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