Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen

Detalhes bibliográficos
Ano de defesa: 2021
Autor(a) principal: Pessoa, Eduardo Rodrigues
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/59199
Resumo: Seminal plasma, in addition to being an important carrier medium for ejaculated sperm, is connected to several metabolites that play a significant role in many biological events and have been linked to possible biomarkers, helping to predict the reproductive potential of selected animals. The present study aimed to evaluate the seminal plasma metabolites of Bos taurus indicus bulls of the Guzerá breed and their associations with semen freezability. Semen from nine adult bulls was collected by electroejaculation, followed by andrological examination and cryopreservation. Afterwards, the semen was thawed and the seminal parameters were evaluated by CASA. Seminal plasma samples from bulls were prepared and then analyzed using gas chromatography combined with mass spectrometry (GC-MS) and multivariate and univariate analyzes. The seminal plasma metabolites and their respective pathways were identified by bioinformatics from Xcalibur and Cytoscape programs and the MetaboAnalyst platform. Sixty-two metabolites were identified in the seminal plasma of the bulls. 1,3-Dioxan-5-ol, stearic acid, cholesteryl myristate, 2-ethylheptanoic acid and isobutylamine were the most abundant metabolites, while heptadecanoic acid, pyrrolidine, nonadecanoic acid, 1-ethyl-1-nitrosourea and ethyl lactate were the least abundant metabolites in the seminal plasma. The multivariate statistical analysis indicated a distinct separation between high and low freezability bulls. The metabolites with the highest score of variable importance in the projection (VIP> 1.0) included propanoic acid, ribose, glycine, heptadecanoic acid and undecanoic acid. Among the compounds, propanoic acid (VIP = 3.5) and ribose (VIP = 2.3) were more abundant in the high freezability group (HF) than in the low freezability group (LF). The t-test analysis suggested that only the concentration of ribose was significant (P = 0.014). This metabolite can influence the reduction of oxidative stress, the repair of DNA damage and the prevention of cellular apoptosis, in addition to serving as a possible source of energy for the body. Thus, ribose can be considered a biomarker of seminal freezability in bulls. As far as it is known, this is the first study to conduct an evaluation of the metabolites in the seminal plasma of Guzerá bulls related to different freezability phenotypes. The findings of this study will serve to assist the development of new research and a better understanding of the mechanisms of sperm cryopreservation.
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spelling Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmenMetabolomic profile of seminal plasma of Guzerá bulls (Bos taurus indicus) with different sperm freezabilityCriopreservaçãoPlasma seminalMetabolômicaTourosGuzeráSeminal plasma, in addition to being an important carrier medium for ejaculated sperm, is connected to several metabolites that play a significant role in many biological events and have been linked to possible biomarkers, helping to predict the reproductive potential of selected animals. The present study aimed to evaluate the seminal plasma metabolites of Bos taurus indicus bulls of the Guzerá breed and their associations with semen freezability. Semen from nine adult bulls was collected by electroejaculation, followed by andrological examination and cryopreservation. Afterwards, the semen was thawed and the seminal parameters were evaluated by CASA. Seminal plasma samples from bulls were prepared and then analyzed using gas chromatography combined with mass spectrometry (GC-MS) and multivariate and univariate analyzes. The seminal plasma metabolites and their respective pathways were identified by bioinformatics from Xcalibur and Cytoscape programs and the MetaboAnalyst platform. Sixty-two metabolites were identified in the seminal plasma of the bulls. 1,3-Dioxan-5-ol, stearic acid, cholesteryl myristate, 2-ethylheptanoic acid and isobutylamine were the most abundant metabolites, while heptadecanoic acid, pyrrolidine, nonadecanoic acid, 1-ethyl-1-nitrosourea and ethyl lactate were the least abundant metabolites in the seminal plasma. The multivariate statistical analysis indicated a distinct separation between high and low freezability bulls. The metabolites with the highest score of variable importance in the projection (VIP> 1.0) included propanoic acid, ribose, glycine, heptadecanoic acid and undecanoic acid. Among the compounds, propanoic acid (VIP = 3.5) and ribose (VIP = 2.3) were more abundant in the high freezability group (HF) than in the low freezability group (LF). The t-test analysis suggested that only the concentration of ribose was significant (P = 0.014). This metabolite can influence the reduction of oxidative stress, the repair of DNA damage and the prevention of cellular apoptosis, in addition to serving as a possible source of energy for the body. Thus, ribose can be considered a biomarker of seminal freezability in bulls. As far as it is known, this is the first study to conduct an evaluation of the metabolites in the seminal plasma of Guzerá bulls related to different freezability phenotypes. The findings of this study will serve to assist the development of new research and a better understanding of the mechanisms of sperm cryopreservation.O plasma seminal, além de ser um importante meio carreador dos espermatozoides ejaculados, relaciona-se com diversos metabólitos que exercem significativo papel em vários eventos biológicos e têm sido vinculados a possíveis biomarcadores, auxiliando a predizer o potencial reprodutivo de animais selecionados. Dessa forma, o presente estudo objetivou avaliar os metabólitos do plasma seminal de touros Bos taurus indicus da raça Guzerá e suas associações com a congelabilidade do sêmen. O sêmen de nove touros adultos foi colhido por eletroejaculação e, em seguida, foi realizado o exame andrológico e a criopreservação. Posteriormente, o sêmen foi descongelado e os parâmetros seminais foram avaliados pelo CASA. As amostras do plasma seminal dos touros foram preparadas e, porteriormente, analisadas com a utilização de cromatografia gasosa aliada à espectrometria de massas (CG-EM) e análises multivariadas e univariadas dos dados obtidos. Os metabólitos do plasma seminal e as suas respectivas vias foram identificados por bioinformática a partir dos programas Xcalibur e Cytoscape com o plug-in Metscape e da plataforma MetaboAnalyst. Sessenta e dois metabólitos foram identificados no plasma seminal dos touros. O 1,3-Dioxan-5-ol, ácido esteárico, miristato de colesterila, ácido 2-etilheptanoico e isobutilamina foram os metabólitos mais abundantes enquanto que o ácido heptadecanoico, pirrolidina, ácido não-adecanoico, 1-etil-1-nitrosoureia e o lactato de etila foram os metabólitos menos abundantes no plasma seminal. A análise estatística multivariada indicou uma separação distinta entre os touros de alta e baixa congelabilidade. Os metabólitos com maior pontuação de importância variável na projeção (VIP > 1,0) incluíram o ácido propanoico, ribose, glicina, ácido heptadecanoico e ácido undecanoico. Entre os compostos, o ácido propanoico (VIP = 3,5) e a ribose (VIP = 2,3) foram mais abundantes no grupo de alta congelabilidade (AC) do que no grupo de baixa congelabilidade (BC). A análise do teste t sugeriu que a concentração de ribose foi a única significativa (P = 0,014). Este metabólito pode influenciar na redução do estresse oxidativo, na reparação de danos ao DNA e na prevenção da apoptose celular, além de servir como possível fonte de energia para o organismo. Desse modo, a ribose pode ser considerada um potencial biomarcador de congelabilidade seminal em touros. Até onde sabemos, nosso estudo é o primeiro a conduzir uma avaliação dos metabólitos no plasma seminal de touros da raça Guzerá relacionados com diferentes fenótipos de congelabilidade. As descobertas deste estudo servirão de auxílio para o desenvolvimento de novas pesquisas e de uma melhor compreensão sobre os mecanismos de criopreservação espermática.Moura, Arlindo de Alencar Araripe NoronhaVasconcelos, Fábio RogerPessoa, Eduardo Rodrigues2021-06-24T19:00:12Z2021-06-24T19:00:12Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfPESSOA, Eduardo Rodrigues. Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen. 2021. 57 f. Dissertação (Mestrado em Zootecnia) – Universidade Federal do Ceará, Fortaleza, 2021.http://www.repositorio.ufc.br/handle/riufc/59199porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-06-24T19:08:18Zoai:repositorio.ufc.br:riufc/59199Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T19:01:41.918341Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
Metabolomic profile of seminal plasma of Guzerá bulls (Bos taurus indicus) with different sperm freezability
title Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
spellingShingle Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
Pessoa, Eduardo Rodrigues
Criopreservação
Plasma seminal
Metabolômica
Touros
Guzerá
title_short Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
title_full Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
title_fullStr Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
title_full_unstemmed Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
title_sort Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen
author Pessoa, Eduardo Rodrigues
author_facet Pessoa, Eduardo Rodrigues
author_role author
dc.contributor.none.fl_str_mv Moura, Arlindo de Alencar Araripe Noronha
Vasconcelos, Fábio Roger
dc.contributor.author.fl_str_mv Pessoa, Eduardo Rodrigues
dc.subject.por.fl_str_mv Criopreservação
Plasma seminal
Metabolômica
Touros
Guzerá
topic Criopreservação
Plasma seminal
Metabolômica
Touros
Guzerá
description Seminal plasma, in addition to being an important carrier medium for ejaculated sperm, is connected to several metabolites that play a significant role in many biological events and have been linked to possible biomarkers, helping to predict the reproductive potential of selected animals. The present study aimed to evaluate the seminal plasma metabolites of Bos taurus indicus bulls of the Guzerá breed and their associations with semen freezability. Semen from nine adult bulls was collected by electroejaculation, followed by andrological examination and cryopreservation. Afterwards, the semen was thawed and the seminal parameters were evaluated by CASA. Seminal plasma samples from bulls were prepared and then analyzed using gas chromatography combined with mass spectrometry (GC-MS) and multivariate and univariate analyzes. The seminal plasma metabolites and their respective pathways were identified by bioinformatics from Xcalibur and Cytoscape programs and the MetaboAnalyst platform. Sixty-two metabolites were identified in the seminal plasma of the bulls. 1,3-Dioxan-5-ol, stearic acid, cholesteryl myristate, 2-ethylheptanoic acid and isobutylamine were the most abundant metabolites, while heptadecanoic acid, pyrrolidine, nonadecanoic acid, 1-ethyl-1-nitrosourea and ethyl lactate were the least abundant metabolites in the seminal plasma. The multivariate statistical analysis indicated a distinct separation between high and low freezability bulls. The metabolites with the highest score of variable importance in the projection (VIP> 1.0) included propanoic acid, ribose, glycine, heptadecanoic acid and undecanoic acid. Among the compounds, propanoic acid (VIP = 3.5) and ribose (VIP = 2.3) were more abundant in the high freezability group (HF) than in the low freezability group (LF). The t-test analysis suggested that only the concentration of ribose was significant (P = 0.014). This metabolite can influence the reduction of oxidative stress, the repair of DNA damage and the prevention of cellular apoptosis, in addition to serving as a possible source of energy for the body. Thus, ribose can be considered a biomarker of seminal freezability in bulls. As far as it is known, this is the first study to conduct an evaluation of the metabolites in the seminal plasma of Guzerá bulls related to different freezability phenotypes. The findings of this study will serve to assist the development of new research and a better understanding of the mechanisms of sperm cryopreservation.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-24T19:00:12Z
2021-06-24T19:00:12Z
2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PESSOA, Eduardo Rodrigues. Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen. 2021. 57 f. Dissertação (Mestrado em Zootecnia) – Universidade Federal do Ceará, Fortaleza, 2021.
http://www.repositorio.ufc.br/handle/riufc/59199
identifier_str_mv PESSOA, Eduardo Rodrigues. Perfil metabolômico do plasma seminal de touros guzerá (Bos taurus indicus) com diferentes parâmetros de congelabilidade do sêmen. 2021. 57 f. Dissertação (Mestrado em Zootecnia) – Universidade Federal do Ceará, Fortaleza, 2021.
url http://www.repositorio.ufc.br/handle/riufc/59199
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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