Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Lima, Joan Petrus Oliveira
Orientador(a): Fechine, Pierre Basílio Almeida
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Hidrogéis
Curcumina
Biomaterial
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/39665
Resumo: This work aims to develop hydrogels from collagen extracted from the skin of Nile Tilapia (Oreochromis niloticus) with synthetic calcium and strontium apatites to produce a potential scaffold cross-linked with curcumin. The extraction of collagen was perfomed in chemical pathway, the synthesis of apatites were by coprecipitation associated to hydrothermal and the hydrogels were obtained by a in situ process. The raw materials and hydrogels were characterized by Fourier-transform infrared spectroscopy (FTIR), swelling, x-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and energy-dispersive spectroscopy (SEM/EDS). The FTIR showed that the apatites are close to the biological due to the presence of HPO42-, collagen presented the typical bands and it’s structural integrity, the hydrogels suffered broadening caused by the crosslinking mechanism. The swelling of the hydrogels in medium similar to the physiological promoted the release of curcumin. The XRD confirmed the hexagonal phase and well-crystallinity of the apatites and a crystalline phase of curcumin. The SEM showed the formation of grains in apatites, fibrous structure and smooth surface of collagen, increase in superface area in hydrogels with curcumin and crystal deposited on the surface of those with apatite. TGA and DSC showed the physic-chemical instability of the hydrogels, supposedly due to the competition of the hydrogen bond sites intra and inter collagenous chains with the solution and curcumin. EDS confirmed the non-stoichiometry of the apatite and the purity of collagen. The hydrogels showed potential as scaffolds with a novel underexplored biocompatible and bioactive crosslinker, with it’s release
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spelling Lima, Joan Petrus OliveiraFechine, Pierre Basílio Almeida2019-02-15T13:15:15Z2019-02-15T13:15:15Z2018LIMA, Joan Petrus Oliveira. Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas. 2018. 69 f. Dissertação (Mestrado em Química)-Universidade Federal do Ceará, Fortaleza, 2018..http://www.repositorio.ufc.br/handle/riufc/39665This work aims to develop hydrogels from collagen extracted from the skin of Nile Tilapia (Oreochromis niloticus) with synthetic calcium and strontium apatites to produce a potential scaffold cross-linked with curcumin. The extraction of collagen was perfomed in chemical pathway, the synthesis of apatites were by coprecipitation associated to hydrothermal and the hydrogels were obtained by a in situ process. The raw materials and hydrogels were characterized by Fourier-transform infrared spectroscopy (FTIR), swelling, x-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and energy-dispersive spectroscopy (SEM/EDS). The FTIR showed that the apatites are close to the biological due to the presence of HPO42-, collagen presented the typical bands and it’s structural integrity, the hydrogels suffered broadening caused by the crosslinking mechanism. The swelling of the hydrogels in medium similar to the physiological promoted the release of curcumin. The XRD confirmed the hexagonal phase and well-crystallinity of the apatites and a crystalline phase of curcumin. The SEM showed the formation of grains in apatites, fibrous structure and smooth surface of collagen, increase in superface area in hydrogels with curcumin and crystal deposited on the surface of those with apatite. TGA and DSC showed the physic-chemical instability of the hydrogels, supposedly due to the competition of the hydrogen bond sites intra and inter collagenous chains with the solution and curcumin. EDS confirmed the non-stoichiometry of the apatite and the purity of collagen. The hydrogels showed potential as scaffolds with a novel underexplored biocompatible and bioactive crosslinker, with it’s releaseCAPESEste trabalho tem como objetivo desenvolver e caracterizar hidrogéis a partir do colágeno de pescado com apatitas de cálcio e estrôncio para o desenvolvimento de arcabouços reticulados com curcumina. A extração do colágeno foi realizada por via química; a síntese das apatitas foi realizada por coprecipitação associada a hidrotermal, e os hidrogéis foram obtidos por um processo in situ. As matérias-primas e os hidrogéis foram caracterizados por espectroscopia no infravermelho por transformada de Fourier (FTIR), entumescimento, difração de Raios-X (DRX), microscopia eletrônica de varredura (MEV), análise termogravimétrica (TGA), calorimetria exploratória diferencial (DSC) e espectroscopia de energia dispersiva (MEV/EDS). O FTIR mostrou que as apatitas são similares às biológicas pela presença de HPO42-; o colágeno apresentou as bandas típicas ressaltando a sua integridade estrutural; os hidrogéis tiveram alargamento das bandas causadas pelo mecanismo de reticulação. O entumescimento dos hidrogéis em meio similar ao fisiológico promoveu a liberação de curcumina. O DRX confirmou a fase hexagonal e a boa cristalinidade das apatitas e uma fase cristalina da curcumina. O MEV mostrou a formação de grãos nas apatitas, a estrutura fibrilar e a superfície lisa do colágeno, o aumento da área superficial dos hidrogéis com curcumina e cristais depositados na superfície nos que possuem apatitas. O TGA e o DSC mostraram instabilidade físico-química dos hidrogéis, supostamente por competição dos sítios de ligação de hidrogênio intra e inter cadeias colágenas com solução e curcumina. O EDS confirmou a não-estequiometria das apatitas e a pureza do colágeno. Os hidrogéis apresentam potencial como arcabouços, com o uso de um agente reticulante inovador, biocompatível e bioativo que é liberado no meio fisiológico.HidrogéisCurcuminaBiomaterialArcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicasScaffolds based on Tilapia (Oreochromis niloticus) skin collagen incorporated with apatites for biomedical applicationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/39665/4/license.txt8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINAL2018_dis_jpolima.pdf2018_dis_jpolima.pdfapplication/pdf695689http://repositorio.ufc.br/bitstream/riufc/39665/5/2018_dis_jpolima.pdfbbeac00b5206857d564b1a1b4bc4b0adMD55riufc/396652019-02-15 10:20:05.377oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2019-02-15T13:20:05Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
dc.title.en.pt_BR.fl_str_mv Scaffolds based on Tilapia (Oreochromis niloticus) skin collagen incorporated with apatites for biomedical applications
title Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
spellingShingle Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
Lima, Joan Petrus Oliveira
Hidrogéis
Curcumina
Biomaterial
title_short Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
title_full Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
title_fullStr Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
title_full_unstemmed Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
title_sort Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas
author Lima, Joan Petrus Oliveira
author_facet Lima, Joan Petrus Oliveira
author_role author
dc.contributor.author.fl_str_mv Lima, Joan Petrus Oliveira
dc.contributor.advisor1.fl_str_mv Fechine, Pierre Basílio Almeida
contributor_str_mv Fechine, Pierre Basílio Almeida
dc.subject.por.fl_str_mv Hidrogéis
Curcumina
Biomaterial
topic Hidrogéis
Curcumina
Biomaterial
description This work aims to develop hydrogels from collagen extracted from the skin of Nile Tilapia (Oreochromis niloticus) with synthetic calcium and strontium apatites to produce a potential scaffold cross-linked with curcumin. The extraction of collagen was perfomed in chemical pathway, the synthesis of apatites were by coprecipitation associated to hydrothermal and the hydrogels were obtained by a in situ process. The raw materials and hydrogels were characterized by Fourier-transform infrared spectroscopy (FTIR), swelling, x-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and energy-dispersive spectroscopy (SEM/EDS). The FTIR showed that the apatites are close to the biological due to the presence of HPO42-, collagen presented the typical bands and it’s structural integrity, the hydrogels suffered broadening caused by the crosslinking mechanism. The swelling of the hydrogels in medium similar to the physiological promoted the release of curcumin. The XRD confirmed the hexagonal phase and well-crystallinity of the apatites and a crystalline phase of curcumin. The SEM showed the formation of grains in apatites, fibrous structure and smooth surface of collagen, increase in superface area in hydrogels with curcumin and crystal deposited on the surface of those with apatite. TGA and DSC showed the physic-chemical instability of the hydrogels, supposedly due to the competition of the hydrogen bond sites intra and inter collagenous chains with the solution and curcumin. EDS confirmed the non-stoichiometry of the apatite and the purity of collagen. The hydrogels showed potential as scaffolds with a novel underexplored biocompatible and bioactive crosslinker, with it’s release
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-15T13:15:15Z
dc.date.available.fl_str_mv 2019-02-15T13:15:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Joan Petrus Oliveira. Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas. 2018. 69 f. Dissertação (Mestrado em Química)-Universidade Federal do Ceará, Fortaleza, 2018..
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/39665
identifier_str_mv LIMA, Joan Petrus Oliveira. Arcabouços à base de colágeno de pele de Tilápia (Oreochromis niloticus) incorporados de apatitas para aplicações biomédicas. 2018. 69 f. Dissertação (Mestrado em Química)-Universidade Federal do Ceará, Fortaleza, 2018..
url http://www.repositorio.ufc.br/handle/riufc/39665
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
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institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/39665/4/license.txt
http://repositorio.ufc.br/bitstream/riufc/39665/5/2018_dis_jpolima.pdf
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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