Vias alternativas de transmissão do Trypanosoma vivax em caprinos infectados experimentalmente

• Ano de defesa: 2017
• Autor(a) principal: Bezerra, Nicholas Morais
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural do Semi-Árido; Brasil; UFERSA; Programa de Pós-Graduação em Ciência Animal
• Palavras-chave em Português: Caprino; Transmissão; Trypanosoma vivax; Goat; Transmission; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: https://doi.org/10.21708/bdtd.ppgca.tese.725; https://repositorio.ufersa.edu.br/handle/tede/725

Whereas some species of trypanosomatids are capable of being transmitted by sexual intercourse and colostrum, these ways of transmission have not been proved yet through the trypanosomiasis caused by trypanosoma vivax. The actual study aimed to research trough the polymerase chain reaction (PCR) the presence of DNA of T. vivax on semen from goats infected by experiments and in the colostrum of infected goats at the last trimester of gestation. It also investigates the possibility of transmission in goats from the parasite through sexual intercourse between the male and female infected goats to those proles by breastfeeding six male goats were used in the experiment to test sexual transmission, which were intravenously infected with 0.5 ml of blood containing approximately 1.25 × 10⁵ trypomastigotes of T. vivax and 6 male goats that were not infected for being a group control. It was collected some samples of semen to the T. Vivax DNA analysis through a polymerase chain reaction (PCR). Six not infected goats by T. vivax, the ones of the Saanen breed, were mated with infected males. In order to analyze the transmission of T. vivax through sexual intercourse, after mating, the goats were submitted to parasitological (blood smear) and molecular (PCR) tests. At the experiment to test the transmission by colostrum were used twelve goats of the Saanen breed were assessed at the trial transmission by colostrum. from this analysis, six goats were infected and the other six ones were analyzed as control to observe the presence of the parasite DNA in the colostrum collected after calving, and later, this colostrum was ministered to newborns from others healthy goats. Then, the same ones were submitted to parasitological and molecular tests. The detection of T. vivax DNA (TviCatL-PCR) on samples of semen by the PCR technique proved to be effective in all those animals and periods of observations. It was also observed the presence of the parasite DNA in some colostrum samples from goats. However, the results for the transmission trials on goats of the first experiment and on newborns breastfeed of the second experiment were negative. Despite the negatives results on transmission, we cannot eliminate the possibility of transmission by sexual intercourse and colostrum since others factors may be involved