Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas
| Ano de defesa: | 2016 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Tese |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal Rural do Semi-Árido
Brasil UFERSA Programa de Pós-Graduação em Fitotecnia |
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Palavras-chave em Português: | |
| Link de acesso: | https://doi.org/10.21708/bdtd.ppgfito.tese.611 https://repositorio.ufersa.edu.br/handle/tede/611 |
Resumo: | The social and economic importance of yellow mombin is due to the marketing of its raw fruits and derivatives. The non-existence of commercial clones and the high plant size are the main obstacles to its rational cultivation being necessary to make the cloning a viable instrument through the interspecific rootstocks in order to downsize the plants heights. Studies done with yellow mombin grafted onto rootstocks of yellow mombin and hog-plum did not markedly downsize the plant height but these clones were not evaluated in other enviroments. The yellow mombin shows potential for remedies. In the pharmaceutical industry it is used as phytotherapics against the Herpes virus. In works done with this species some bioactive compounds were found in the leaves such as flavonoids and tannins. Although this potential, some researches on chemical characterization and genetic diversity among genotypes are incipient. The objective of this study was to evaluate the vegetative growth of yellow mombin clones grafted onto interspecific rootstocks in different environments and the genetic diversity as to bioactive compounds among accessions of the Emepa-PB´s germplasm bank. In order to evaluate the clones development two trials with a completely randomized block design were performed. The trial 1 was conducted in a 3 x 4 x 3 factorial design with four repetitions, totaling 36 treatments, being three different interspecific rootstocks with yellow mombin (S. mombin), hog-plum (S. tuberosa) and coarse mombin (S. venulosa), four graftings of yellow mombin clones (Itaitinga, Gereau, Lagoa Redonda and Genipabu) and three different places (João Pessoa, PB, Ipanguaçu, RN and Itabuna, BA). The trial 2 was conducted in a 4 x 3 factorial design, with four repetitions, totaling 12 treatments. Three graftings of yellow mombin (Emepa 8.2, Emepa 8.6 and Emepa 20) obtained from the grafting between yellow mombin clones, grafted onto yellow mombin, plus one genotype of ungrafted yellow mombin, were used in the same places. The evaluated variables for the two trials at the 8, 39 and 46 months old were plant height, stem circumference, crown spread, crown format, number of branches and percentage of dead plants. The conclusions are that the environment, the grafting and the rootstocks did not influence the size and vigor of the clones allowing the identification of the morphological characteristics variations in each environment; that in Trial 1, for the three enviroments, the graftings of yellow mombin onto yellow mombin, hog-plum and coarse mombin rootstocks did not form clones with ideal sizes so that they cannot be recommended for commercial plantation; that in Trial 2, in the three enviroments, the Emepa 8.2 showed smaller size, bigger crown spread, major number of branches, 100% crown in a spread format and it can be recommended for new studies and later multiplication for commercial plantation. Leaves and barks of 27 yellow mombin accessions of the Emepa´s germplasm bank, in João Pessoa, PB, were used. The extractions were performed with methanol and the identification and quantification of bioactive compounds were checked through the High-Performance Liquid Chromatography (HPLC). For the grouping of accessions were used the Principal Components Analysis (PCA) and the UPGMA method. The standardized Euclidean distancewas used as a measure of genetic diversity. Rutin, quercetin, ellagic acid and geraniin compounds were quantified in leaves and ellagic acid and geraniin compounds were quantified inbarks. The highest amounts were found in leaves. Seven groups were formed. The accession group A3 was the most divergent with high amounts of rutin, quercetin and ellagic acid in leaves. The conclusion is that there is variability among accessions and it must be preserved and exploited through the genetic improvement programs |
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Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhasDevelopment of yellow mombin clones onto different rootstocks and diversity of genetic accessions as to the bioactive compounds in the bark and leavesPorta-enxerto interespecíficoClones copaVariabilidade genéticaÁcido elágicoRutinaQuercetinaGeraniinaInterspecific rootstockScion clonesGenetic variabilityRutinQuercetinEllagic acidGeraniinCIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIAThe social and economic importance of yellow mombin is due to the marketing of its raw fruits and derivatives. The non-existence of commercial clones and the high plant size are the main obstacles to its rational cultivation being necessary to make the cloning a viable instrument through the interspecific rootstocks in order to downsize the plants heights. Studies done with yellow mombin grafted onto rootstocks of yellow mombin and hog-plum did not markedly downsize the plant height but these clones were not evaluated in other enviroments. The yellow mombin shows potential for remedies. In the pharmaceutical industry it is used as phytotherapics against the Herpes virus. In works done with this species some bioactive compounds were found in the leaves such as flavonoids and tannins. Although this potential, some researches on chemical characterization and genetic diversity among genotypes are incipient. The objective of this study was to evaluate the vegetative growth of yellow mombin clones grafted onto interspecific rootstocks in different environments and the genetic diversity as to bioactive compounds among accessions of the Emepa-PB´s germplasm bank. In order to evaluate the clones development two trials with a completely randomized block design were performed. The trial 1 was conducted in a 3 x 4 x 3 factorial design with four repetitions, totaling 36 treatments, being three different interspecific rootstocks with yellow mombin (S. mombin), hog-plum (S. tuberosa) and coarse mombin (S. venulosa), four graftings of yellow mombin clones (Itaitinga, Gereau, Lagoa Redonda and Genipabu) and three different places (João Pessoa, PB, Ipanguaçu, RN and Itabuna, BA). The trial 2 was conducted in a 4 x 3 factorial design, with four repetitions, totaling 12 treatments. Three graftings of yellow mombin (Emepa 8.2, Emepa 8.6 and Emepa 20) obtained from the grafting between yellow mombin clones, grafted onto yellow mombin, plus one genotype of ungrafted yellow mombin, were used in the same places. The evaluated variables for the two trials at the 8, 39 and 46 months old were plant height, stem circumference, crown spread, crown format, number of branches and percentage of dead plants. The conclusions are that the environment, the grafting and the rootstocks did not influence the size and vigor of the clones allowing the identification of the morphological characteristics variations in each environment; that in Trial 1, for the three enviroments, the graftings of yellow mombin onto yellow mombin, hog-plum and coarse mombin rootstocks did not form clones with ideal sizes so that they cannot be recommended for commercial plantation; that in Trial 2, in the three enviroments, the Emepa 8.2 showed smaller size, bigger crown spread, major number of branches, 100% crown in a spread format and it can be recommended for new studies and later multiplication for commercial plantation. Leaves and barks of 27 yellow mombin accessions of the Emepa´s germplasm bank, in João Pessoa, PB, were used. The extractions were performed with methanol and the identification and quantification of bioactive compounds were checked through the High-Performance Liquid Chromatography (HPLC). For the grouping of accessions were used the Principal Components Analysis (PCA) and the UPGMA method. The standardized Euclidean distancewas used as a measure of genetic diversity. Rutin, quercetin, ellagic acid and geraniin compounds were quantified in leaves and ellagic acid and geraniin compounds were quantified inbarks. The highest amounts were found in leaves. Seven groups were formed. The accession group A3 was the most divergent with high amounts of rutin, quercetin and ellagic acid in leaves. The conclusion is that there is variability among accessions and it must be preserved and exploited through the genetic improvement programs2017-02-24A importância socioeconômica da cajazeira é devido à comercialização dos frutos e do consumo de seus derivados. A inexistência de clones comerciais e o porte alto da planta constituem-se em entraves ao seu cultivo racional, necessitando viabilizar a clonagem com porta-enxertos interspecíficos visando reduzir o porte das plantas. Estudos realizados com a cajazeira enxertada na própria espécie e em umbuzeiro não demonstraram a diminuição significativa do porte da planta. No entanto, esses clones não foram avaliados em outros ambientes. A cajazeira apresenta potencial para medicamentos. Na indústria farmacêutica é utilizada como fitoterápico contra o vírus do Herpes. Em trabalhos realizados por outros pesquisadores, foram identificados compostos bioativos nas folhas tais como flavonoides e taninos. Apesar desse potencial, as pesquisas sobre a caracterização química e a divergência genética entre os genótipos são incipientes. O objetivo deste trabalho foi avaliar o desenvolvimento de clones de cajazeira enxertados sobre porta-enxertos interespecíficos em diferentes ambientes e a diversidade genética quanto a compostos bioativos entre acessos do banco de germoplasma da Emepa-PB. Para avaliação do desenvolvimento dos clones, foram realizados dois ensaios em delineamento inteiramente casualizado. O Ensaio 1 foi conduzido em esquema fatorial 3 x 4 x 3, com quatro repetições, totalizando 36 tratamentos, sendo três porta-enxertos interespecíficos com cajazeira (S. mombin), umbuzeiro (S. tuberosa) e cajazeira-grande (S. venulosa), quatro enxertos de clones de cajazeira (Itaitinga, Gereau, Lagoa Redonda e Genipabu), e três locais (João Pessoa, PB, Ipanguaçu, RN e Itabuna, BA). O Ensaio 2 foi conduzido em esquema fatorial 4 x 3, com quatro repetições, totalizando 12 tratamentos. Foram utilizados três exertos de acessos de cajazeira (Emepa 8.2, Emepa 8.6 e Emepa 20) enxertados sobre cajazeira, mais um pé-franco de cajazeira, nos mesmos locais. As variáveis avaliadas, aos 8, 39 e 46 meses de idade, para os Ensaios 1 e 2, foram altura de planta, perímetro de caule, largura e formato de copa, número de ramos e mortalidade de plantas. Concluiu-se que o ambiente, os enxertos e os porta-enxertos de espécies de Spondias influenciaram no desenvolvimento dos clones, permitindo identificar variabilidade nas características morfológicas em cada ambiente; no Ensaio 1, nos três ambientes, a enxertia da cajazeira sobre porta-enxertos de cajazeira, cajazeira-grande e umbuzeiro não formaram clones com porte ideal para recomendação comercial; no Ensaio 2, nos três ambientes, o clone Emepa 8.2 apresentou menor porte, maior largura de copa, maior número de ramos e 100% de formato de copa esgalhada, podendo ser recomendado para novos estudos e posterior multiplicação para exploração comercial. Para avaliação da diversidade genética, foram utilizadas folhas e cascas de cajazeira de 27 acessos do banco de germoplasma da Emepa, em João Pessoa, PB. As extrações foram realizadas com metanol e a quantificação foi determinada por Cromatografia Líquida de Alta Eficiência (CLAE). Para o agrupamento dos acessos utilizou-se a Análise de Componentes Principais (ACP) e o método aglomerativo UPGMA. Foi utilizada a distância euclidiana média padronizada como medida de dissimilaridade genética. Foram quantificados nas folhas os compostos rutina, quercetina, ácido elágico e geraniina e foram quantificados nas cascas os compostos ácido elágico e geraniina. Os maiores valores foram obtidos nas folhas. Foram formados 7 grupos. O acesso A3 foi o mais divergente com valores elevados de rutina, quercetina e geraniina nas folhas. Concluiu-se que há variabilidade entre os acessos e que esta deve ser preservada e explorada em programas de melhoramento genéticoEmepa - Empresa Estadual de Pesquisa AgropecuáriaUniversidade Federal Rural do Semi-ÁridoBrasilUFERSAPrograma de Pós-Graduação em FitotecniaSouza, Francisco Xavier de15517853404http://lattes.cnpq.br/4014849457129237Harand, Wolfgang01539667421http://lattes.cnpq.br/3627883035710562Alves, Ricardo Elesbão46764429472http://lattes.cnpq.br/658093491002017567465072549http://lattes.cnpq.br/3099971303096203Mendonca, Vander66616620697http://lattes.cnpq.br/4327895435746416Nunes, Glauber Henrique de Sousa82888884453http://lattes.cnpq.br/6199153570472953Zocolo, Guilherme Julião03661154648http://lattes.cnpq.br/4626560439053690Aragão, Fernando Antonio Souza de37135708334http://lattes.cnpq.br/9233471904227470Ramires, Christiane Mendes Cassimiro2017-03-21T15:02:28Z2016-03-03info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfRAMIRES, Christiane Mendes Cassimiro. Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas. 2016. 164 f. Tese (Doutorado) - Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Semi-Árido, Mossoró, 2016.https://doi.org/10.21708/bdtd.ppgfito.tese.611https://repositorio.ufersa.edu.br/handle/tede/611porinfo:eu-repo/semantics/openAccessCC-BY-SAreponame:Repositório Digital da Universidade Federal Rural do Semiárido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2024-11-27T20:38:29Zoai:repositorio.ufersa.edu.br:tede/611Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2024-11-27T20:38:29Repositório Digital da Universidade Federal Rural do Semiárido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
| dc.title.none.fl_str_mv |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas Development of yellow mombin clones onto different rootstocks and diversity of genetic accessions as to the bioactive compounds in the bark and leaves |
| title |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas |
| spellingShingle |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas Ramires, Christiane Mendes Cassimiro Porta-enxerto interespecífico Clones copa Variabilidade genética Ácido elágico Rutina Quercetina Geraniina Interspecific rootstock Scion clones Genetic variability Rutin Quercetin Ellagic acid Geraniin CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
| title_short |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas |
| title_full |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas |
| title_fullStr |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas |
| title_full_unstemmed |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas |
| title_sort |
Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas |
| author |
Ramires, Christiane Mendes Cassimiro |
| author_facet |
Ramires, Christiane Mendes Cassimiro |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
Souza, Francisco Xavier de 15517853404 http://lattes.cnpq.br/4014849457129237 Harand, Wolfgang 01539667421 http://lattes.cnpq.br/3627883035710562 Alves, Ricardo Elesbão 46764429472 http://lattes.cnpq.br/6580934910020175 67465072549 http://lattes.cnpq.br/3099971303096203 Mendonca, Vander 66616620697 http://lattes.cnpq.br/4327895435746416 Nunes, Glauber Henrique de Sousa 82888884453 http://lattes.cnpq.br/6199153570472953 Zocolo, Guilherme Julião 03661154648 http://lattes.cnpq.br/4626560439053690 Aragão, Fernando Antonio Souza de 37135708334 http://lattes.cnpq.br/9233471904227470 |
| dc.contributor.author.fl_str_mv |
Ramires, Christiane Mendes Cassimiro |
| dc.subject.por.fl_str_mv |
Porta-enxerto interespecífico Clones copa Variabilidade genética Ácido elágico Rutina Quercetina Geraniina Interspecific rootstock Scion clones Genetic variability Rutin Quercetin Ellagic acid Geraniin CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
| topic |
Porta-enxerto interespecífico Clones copa Variabilidade genética Ácido elágico Rutina Quercetina Geraniina Interspecific rootstock Scion clones Genetic variability Rutin Quercetin Ellagic acid Geraniin CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA |
| description |
The social and economic importance of yellow mombin is due to the marketing of its raw fruits and derivatives. The non-existence of commercial clones and the high plant size are the main obstacles to its rational cultivation being necessary to make the cloning a viable instrument through the interspecific rootstocks in order to downsize the plants heights. Studies done with yellow mombin grafted onto rootstocks of yellow mombin and hog-plum did not markedly downsize the plant height but these clones were not evaluated in other enviroments. The yellow mombin shows potential for remedies. In the pharmaceutical industry it is used as phytotherapics against the Herpes virus. In works done with this species some bioactive compounds were found in the leaves such as flavonoids and tannins. Although this potential, some researches on chemical characterization and genetic diversity among genotypes are incipient. The objective of this study was to evaluate the vegetative growth of yellow mombin clones grafted onto interspecific rootstocks in different environments and the genetic diversity as to bioactive compounds among accessions of the Emepa-PB´s germplasm bank. In order to evaluate the clones development two trials with a completely randomized block design were performed. The trial 1 was conducted in a 3 x 4 x 3 factorial design with four repetitions, totaling 36 treatments, being three different interspecific rootstocks with yellow mombin (S. mombin), hog-plum (S. tuberosa) and coarse mombin (S. venulosa), four graftings of yellow mombin clones (Itaitinga, Gereau, Lagoa Redonda and Genipabu) and three different places (João Pessoa, PB, Ipanguaçu, RN and Itabuna, BA). The trial 2 was conducted in a 4 x 3 factorial design, with four repetitions, totaling 12 treatments. Three graftings of yellow mombin (Emepa 8.2, Emepa 8.6 and Emepa 20) obtained from the grafting between yellow mombin clones, grafted onto yellow mombin, plus one genotype of ungrafted yellow mombin, were used in the same places. The evaluated variables for the two trials at the 8, 39 and 46 months old were plant height, stem circumference, crown spread, crown format, number of branches and percentage of dead plants. The conclusions are that the environment, the grafting and the rootstocks did not influence the size and vigor of the clones allowing the identification of the morphological characteristics variations in each environment; that in Trial 1, for the three enviroments, the graftings of yellow mombin onto yellow mombin, hog-plum and coarse mombin rootstocks did not form clones with ideal sizes so that they cannot be recommended for commercial plantation; that in Trial 2, in the three enviroments, the Emepa 8.2 showed smaller size, bigger crown spread, major number of branches, 100% crown in a spread format and it can be recommended for new studies and later multiplication for commercial plantation. Leaves and barks of 27 yellow mombin accessions of the Emepa´s germplasm bank, in João Pessoa, PB, were used. The extractions were performed with methanol and the identification and quantification of bioactive compounds were checked through the High-Performance Liquid Chromatography (HPLC). For the grouping of accessions were used the Principal Components Analysis (PCA) and the UPGMA method. The standardized Euclidean distancewas used as a measure of genetic diversity. Rutin, quercetin, ellagic acid and geraniin compounds were quantified in leaves and ellagic acid and geraniin compounds were quantified inbarks. The highest amounts were found in leaves. Seven groups were formed. The accession group A3 was the most divergent with high amounts of rutin, quercetin and ellagic acid in leaves. The conclusion is that there is variability among accessions and it must be preserved and exploited through the genetic improvement programs |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-03-03 2017-03-21T15:02:28Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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info:eu-repo/semantics/publishedVersion |
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doctoralThesis |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
RAMIRES, Christiane Mendes Cassimiro. Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas. 2016. 164 f. Tese (Doutorado) - Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. https://doi.org/10.21708/bdtd.ppgfito.tese.611 https://repositorio.ufersa.edu.br/handle/tede/611 |
| identifier_str_mv |
RAMIRES, Christiane Mendes Cassimiro. Desenvolvimento de clones de cajazeiras sobre diferentes porta-enxertos e diversidade genética de acessos quanto a compostos bioativos nas cascas e folhas. 2016. 164 f. Tese (Doutorado) - Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. |
| url |
https://doi.org/10.21708/bdtd.ppgfito.tese.611 https://repositorio.ufersa.edu.br/handle/tede/611 |
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por |
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openAccess |
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CC-BY-SA |
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application/pdf application/pdf |
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Universidade Federal Rural do Semi-Árido Brasil UFERSA Programa de Pós-Graduação em Fitotecnia |
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Universidade Federal Rural do Semi-Árido Brasil UFERSA Programa de Pós-Graduação em Fitotecnia |
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Repositório Digital da Universidade Federal Rural do Semiárido (RDU) |
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Repositório Digital da Universidade Federal Rural do Semiárido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
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